"Goldberg Validations": Refractometric Protein Concentration Calibration of Pooled Source Plasma and Validation of Clinical Serum Protein Refractometer

Total protein-bound hexose, hexosamine, and sialic acid were determined in sera of six littermate mongrel pups at monthly intervals from the 4th day after birth up to the age of 7 months. The concentration of the individual constituents fluctuated considerably from month to month, but the values showed neither a definite trend nor a relationship to weight gain. When the carbohydrate moiety was expressed as percentage of total serum protein concentration, the values were high in newborn pups and diminished after the 1st month of life. This was due to a rise in the concentration of total serum protein, brought about by an increase of the albumin fraction which is low in carbohydrate.

Download Full-text

Effect of protein concentration on the determination of digoxin in serum by fluorescence polarization immunoassay.

Clinical Chemistry ◽

10.1093/clinchem/30.11.1826 ◽

1984 ◽

Vol 30 (11) ◽

pp. 1826-1829 ◽

Cited By ~ 13

Author(s):

W H Porter ◽

V M Haver ◽

B A Bush

Keyword(s):

Serum Protein ◽

Total Protein ◽

Fluorescence Polarization ◽

Protein Concentration ◽

Total Serum Protein ◽

Total Serum ◽

Fluorescence Polarization Immunoassay ◽

Total Protein Concentration ◽

Protein Pellet

Abstract Determination of digoxin by fluorescence polarization immunoassay (FPIA) with the Abbott "TDx" is significantly influenced by the concentration of total serum protein. Each 10 g/L increase in serum protein results in an 8% decrease in measured digoxin. Studies with [3H]digoxin confirmed that digoxin binds to the protein pellet during the trichloroacetic acid precipitation step before the immunoassay. Serum protein, or equal concentrations of albumin or gamma-globulin, exert an equivalent effect on the apparent digoxin value. Because the total protein concentration of the assay calibrators is low (50 g/L) compared with its reference interval in serum (60-80 g/L), results by FPIA may be expected to be low by an average of 16% (range, 8-24%). Digoxin results by FPIA will be most nearly accurate when the calibrators include a total protein concentration of about 70 g/L. Patients' specimens with abnormally high or low protein content will give falsely high or low results for digoxin.

Download Full-text

Measurement of Monoclonal Immunoglobulin Protein Concentration in Serum Protein Electrophoresis: Comparison of Automated vs Manual/Human Readings

Laboratory Medicine ◽

10.1093/labmed/lmz055 ◽

2019 ◽

Vol 51 (3) ◽

pp. 252-258 ◽

Cited By ~ 2

Author(s):

Alex Clavijo ◽

Nathan Ryan ◽

Hongyan Xu ◽

Gurmukh Singh

Keyword(s):

Serum Protein ◽

Protein Concentration ◽

Protein Electrophoresis ◽

Serum Protein Electrophoresis ◽

Response To Treatment ◽

Computer Assisted ◽

Positive Bias ◽

Monoclonal Immunoglobulin ◽

Monoclonal Immunoglobulins ◽

Immunofixation Electrophoresis

Abstract Background Protein concentration of monoclonal immunoglobulin in plasma-cell myeloma/multiple myeloma provides an estimate of the tumor mass and allows for monitoring of the response to treatment. Accurate and reproducible estimates of the monoclonal immunoglobulin concentration are important for patient care. Objective To address the optimum method for estimation of the concentration of monoclonal immunoglobulins. Methods Serum protein electrophoresis and immunofixation electrophoresis were conducted by using the Helena SPIFE Touch instrument. Estimation of the protein concentration of monoclonal immunoglobulin in the gamma region by computer-assisted reading was compared with the reading by technologists and pathology residents, in 300 gels. The data were compared using t-testing and analysis of variance. Results Computer-generated readings had a consistent positive bias. The correlation coefficient of the average reading by technologists and residents with the computer generated value was 0.997. The average positive bias by the computer reading was 0.29 g per dL. The intercept on the regression analysis was 0.22 g per dL. The reading by the computer was significantly higher than each of the human-interpreted readings. The readings by the 3 human groups were not significantly different amongst them. The main reason for the higher reading by the computer was inclusion of a greater area on the anodal size of the peak on the densitometric scan. Conclusions Human- and computer-interpreted readings of the protein concentration of monoclonal immunoglobulin have a high degree of correlation. The consistent positive bias by the computer reading occurred due to inclusion of a greater area of the densitometric scan on the anodal side of the peak. We suggest that vendors should adjust such computer programs to provide readings comparable to those generated by expert humans. We recommend manual delineation of the monoclonal peaks for measuring the concentration of monoclonal immunoglobulins.

Download Full-text

Some immunological studies on a new vaccine derived frombacteriophage lysate of Pasteurella multocida in mice

Indian Journal of Animal Research ◽

10.18805/ijar.v0iof.7000 ◽

2017 ◽

Author(s):

Hari Mohan Saxena ◽

Yanglem Pushpa ◽

Sabia Qureshi

Keyword(s):

Immune Response ◽

Serum Protein ◽

Humoral Immune Response ◽

Protein Concentration ◽

Pasteurella Multocida ◽

Phage Lysate ◽

Serum Globulin ◽

Humoral Immune ◽

Serum Igg Levels ◽

Serum Protein Concentration

The study was undertaken to evaluate the humoral immune response in mice induced by a novel phage lysate vaccine against Hemorrhagic Septicemia developed from Pasteurella multocida grown under iron-restricted conditions and lysed by a bacteriophage. Two groups of mice were immunized with lysate vaccine (LV) and conventional killed HS vaccine (CV) and blood was collected at various days post – immunization (DPI). The serum protein concentration in lysate vaccinated mice (4.675±0.223) was significantly higher (p less than 0.05) than the conventional vaccinated mice (4.100±0.282) at 150 DPI. The serum globulin levels at 90 DPI and 180 DPI in LV mice (1.330±0.071 and 0.650±0.100) were significantly (p less than 0.01) higher than the CV mice (0.850±0.084 and 0.366±0.098). The serum IgG levels at 150 DPI and 180 DPI in LV (0.564±0.188 and 0.485±0121) mice were significantly higher (p less than 0.01) than the CV mice (0.178±0.039 and 0.121±0.026). Although our results in mice are promising, further studies involving bovines are needed.

Download Full-text

SERUM PROTEIN CONCENTRATION AND SOMATIC INDEX IN RELATION TO MORPHOMETRIC MATURITY FOR MALE SNOW CRAB, CHIONOECETES OPILIO (O. FABRICIUS, 1788), IN THE BAIE DES CHALEURS, CANADA (DECAPODA, BRACHYURA)

Crustaceana ◽

10.1163/156854099503546 ◽

1999 ◽

Vol 72 (5) ◽

pp. 497-506 ◽

Cited By ~ 2

Author(s):

Michel Comeau ◽

Marc Lanteigne ◽

Roland Cormier

Keyword(s):

Serum Protein ◽

Protein Concentration ◽

Mature Male ◽

Snow Crab ◽

Juvenile Male ◽

Chionoecetes Opilio ◽

Wet Weight ◽

Snow Crabs ◽

Somatic Index ◽

Serum Protein Concentration

AbstractThe serum protein concentrations of juvenile and mature male snow crabs, Chionoecetes opilio, were measured using a refractometer. The somatic indices of juvenile and mature male snow crab were calculated using the weight of the dry flesh of the chelae versus its wet weight. Results indicate that juvenile male snow crab have significantly higher serum protein concentrations and significantly lower somatic indices than their mature counterparts. These observations are attributed to basic physiological differences that could be explained by a terminal moult. Les concentrations en proteines seriques de males juveniles et a maturite du crabe des neiges Chionoecetes opilio ont ete mesurees au moyen d'un refractometre. Les indices somatiques de ces individus ont ete calcules en utilisant le rapport entre le poids sec et le poids humide de la chair. Les resultats indiquent que les individus males juveniles ont des concentrations en proteines seriques significativement plus elevees et des indices somatiques significativement plus bas que les individus males matures. Ces observations sont attribuees a des differences physiologiques de base qui peuvent etre expliquees par une mue terminale.

Download Full-text