scholarly journals ALTERATIONS IN TOTAL PROTEIN CONCENTRATION, SERUM PROTEIN FRACTIONS AND ALBUMIN/GLOBULIN RATIO IN HEALTHY RABBITS

2013 ◽  
Vol 4 (8) ◽  
pp. 128-130 ◽  
Author(s):  
Nuzhat Sultana ◽  
Rahila Najam
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Protein Concentration ◽  
Protein Fractions ◽  
Total Protein Concentration

Effect of protein concentration on the determination of digoxin in serum by fluorescence polarization immunoassay.

1984 ◽  
Vol 30 (11) ◽  
pp. 1826-1829 ◽  
Author(s):  
W H Porter ◽  
V M Haver ◽  
B A Bush
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Fluorescence Polarization ◽  
Protein Concentration ◽  
Total Serum Protein ◽  
Total Serum ◽  
Fluorescence Polarization Immunoassay ◽  
Total Protein Concentration ◽  
Protein Pellet

Abstract Determination of digoxin by fluorescence polarization immunoassay (FPIA) with the Abbott "TDx" is significantly influenced by the concentration of total serum protein. Each 10 g/L increase in serum protein results in an 8% decrease in measured digoxin. Studies with [3H]digoxin confirmed that digoxin binds to the protein pellet during the trichloroacetic acid precipitation step before the immunoassay. Serum protein, or equal concentrations of albumin or gamma-globulin, exert an equivalent effect on the apparent digoxin value. Because the total protein concentration of the assay calibrators is low (50 g/L) compared with its reference interval in serum (60-80 g/L), results by FPIA may be expected to be low by an average of 16% (range, 8-24%). Digoxin results by FPIA will be most nearly accurate when the calibrators include a total protein concentration of about 70 g/L. Patients' specimens with abnormally high or low protein content will give falsely high or low results for digoxin.

Evaluation of serum total protein concentration and protein fractions in sheep naturally infected with Babesia ovis

2012 ◽  
Vol 23 (1) ◽  
pp. 151-155 ◽  
Author(s):  
B. Esmaeilnejad ◽  
M. Tavassoli ◽  
S. Asri-Rezaei ◽  
B. Dalir-Naghadeh ◽  
S. H. Pourseyed
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Protein Fractions ◽  
Total Protein Concentration ◽  
Serum Total Protein ◽  
Babesia Ovis

scholarly journals THE EFFECT OF FASTING ON THE SERUM PROTEIN CONCENTRATION OF THE RAT

1935 ◽  
Vol 62 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Harold C. Torbert
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Protein Fraction ◽  
Protein Concentration ◽  
Individual Resistance ◽  
Total Protein Concentration ◽  
Albumin Fraction ◽  
Large Numbers ◽  
Initial Drop ◽  
Serum Protein Concentration

1. Experiments were carried out to test the hypothesis that there exists a special circulating fraction of plasma protein available for use by the tissues. 2. The changes in serum protein concentration after varying periods of fasting were followed in large numbers of individual rats. 3. Previous reports from this laboratory of a small initial drop in the total protein concentration of the serum, with subsequent maintenance of the serum protein at the new level are confirmed. 4. Evidence is offered that this initial fall involves chiefly or solely the albumin fraction. 5. The mechanism responsible for the observed initial drop and subsequent maintenance of the protein is not exactly known, but two definite factors are age and individual resistance. 6. It is concluded that no satisfactory evidence is available to support the hypothesis of a directly utilizable protein fraction in the blood.

scholarly journals Total protein, selected protein fractions and chemical elements in the colostrum and milk of mares (Short Communication)

2009 ◽  
Vol 52 (1) ◽  
pp. 1-6
Author(s):  
A. Cieśla ◽  
R. Palacz ◽  
J. Janiszewska ◽  
D. Skórka
Keyword(s):  
Environmental Conditions ◽  
Total Protein ◽  
Protein Fraction ◽  
Protein Concentration ◽  
Short Communication ◽  
Chemical Elements ◽  
Protein Fractions ◽  
Total Protein Concentration ◽  
Dynamic Changes ◽  
Milk Samples

Abstract. The study material consisted of the colostrum and milk of five noble half-blood mares housed under the same environmental conditions. Colostrum samples were collected 24 h after foaling, whereas milk samples were taken five times, every 30 days, with the first sampling on lactation day 30 (1st month). In the samples were determined the concentrations of total protein, pre-albumins, albumins, α- and β-globulins, immunoglobulins and calcium (Ca), magnesium (Mg) and zinc (Zn). The presence of pre-albumins was observed only in mare colostrum. Total protein concentration was at the same level in the colostrum and in the milk in the 1st lactation month, whereas protein fraction concentrations underwent dynamic changes during the 1st month of lactation. When compared to the values determined in the colostrum, the level of α-globulins increased 8 times during lactation, whereas that of immunoglobulins decreased almost 4 times. The highest concentrations of Ca, Mg and Zn were found in mare colostrum, with a decrease in the concentration of all chemical elements in the 1st month of lactation, confirmed statistically in case of Mg and Zn.

Determination of Total Protein Concentration in Solution Using Gold Electrode Modified with Silver Nanoparticles

2014 ◽  
Vol 27 (1) ◽  
pp. 253-257 ◽  
Author(s):  
Patrick Marcel Seumo Tchekwagep ◽  
Charles Péguy Nanseu-Njiki ◽  
Emmanuel Ngameni ◽  
Ravi Danielsson ◽  
Thomas Arnebrant ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Total Protein ◽  
Gold Electrode ◽  
Protein Concentration ◽  
Total Protein Concentration

Albumin fraction and measurement of total protein concentration

1993 ◽  
Vol 264 (5) ◽  
pp. H1723-H1726 ◽  
Author(s):  
B. T. Peterson ◽  
R. W. Tate
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Gamma Globulin ◽  
Colorimetric Assay ◽  
Full Range ◽  
Computational Method ◽  
Total Protein Concentration ◽  
Albumin Fraction ◽  
Standard Curve ◽  
Protein Assays

The standard curve of a typical colorimetric assay for total protein is often nonlinear and dependent on the albumin fraction of the protein standard. We developed a simple mathematical transformation to make the standard curve linear and a computational method to correct for differences in albumin concentrations among the samples. This method uses data from total protein assays on two sets of standards (albumin and gamma globulin) and provides accurate measures of total protein over the full range of albumin fractions. Comparison of this two-standard method with the a method that uses only albumin as a standard shows that this method prevents physiologically significant overestimations in total protein concentration and calculated protein osmotic pressure differences in the lungs.

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