Control biológico de garrapata (Boophilus spp.) con diferentes cepas de Metarhizium anisopliae (Metchnikoff) Sorokin en bovinos

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
López Osorio
Keyword(s):  
Metarhizium Anisopliae

Con el objetivo de evaluar cuatro cepas del hongo Metarhizium anisopliae (Metschincoff) Sorokin para el control garrapatas del genero Boophilus spp., se seleccionaron dos cepas de garrapatas para su evaluación en su fase adulta sobre terneros parasitados. Se utilizó un diseño completamente al azar, con seis tratamientos y diez repeticiones.En esta fase las cepas PLH y CH 93-3 fueron las que mostraron la mayor efectividad, por lo que fueron evaluadas en garrapatas adultas sobre terneros previamente parasitados; en la prueba in vivo se utilizó un diseño de bloques completamente al azar, cuatro tratamientos (Las dos cepas seleccionadas, un compuesto químico y un placebo a base de agua) y ocho repeticiones. Los resultados mostraron que las dos cepas evaluadas presentaron una efectividad del 50% con respecto al producto químico.

Effects of passaging through scarabeid hosts on virulence and host specificity of two strains of the entomopathogenic hyphomycete Metarhizium anisopliae

1983 ◽  
Vol 29 (5) ◽  
pp. 576-583 ◽  
Author(s):  
Jacques F. Fargues ◽  
Pierre H. Robert
Keyword(s):  
Host Species ◽  
Metarhizium Anisopliae ◽  
Insect Host ◽  
Artificial Medium ◽  
Susceptible Host ◽  
Single Host ◽  
Entomopathogenic Hyphomycete ◽  
Enzymatic Mechanisms ◽  
Phenotypic Responses

The effects on pathogenicity of Metarhizium anisopliae (Metsch.) Sor. following one or more passages through the original and the heterologous insect host species were investigated by using two pathotypes specific for Cetonia aurata L. and Oryctes rhinoceros L., respectively. A significant increase in virulence occurred after a single host passage. Thus, the infection potential of host-passed inocula could be increased by a factor of 10 to 100, and its host-induced adaptation was almost complete after the first in vivo passage. Bacteria located on the integument of the host cadaver were not implicated in this phenomenon. When the fungus was again grown on artificial medium, the loss in virulence was also very fast. Consequently, changes in virulence of the two tested strains seemed to be phenotypic responses implicating inducible enzymatic mechanisms. Nevertheless, the parasitic behaviour of the two pathotypes toward the larvae of the nonsusceptible species was not modified by host passaging through their respective insect hosts. It is concluded that changes in entomopathogenic hyphomycete virulence, resulting from host passage, concern only the naturally susceptible host species.

Different strategies to kill the host presented byMetarhizium anisopliaeandBeauveria bassiana

2018 ◽  
Vol 64 (3) ◽  
pp. 191-200 ◽  
Author(s):  
Cynthia Barbosa Rustiguel ◽  
María Fernández-Bravo ◽  
Luis Henrique Souza Guimarães ◽  
Enrique Quesada-Moraga
Keyword(s):  
Secondary Metabolites ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Galleria Mellonella ◽  
Insect Pests ◽  
Fungal Growth ◽  
Growth Strategy

Studies conducted over the last decades have shown the potential of entomopathogenic fungi for the biocontrol of some insect pests. Entomopathogenic fungi infect their host through the cuticle, so they do not need to be ingested to be effective. These fungi also secrete secondary metabolites and proteins that are toxic to insect pests. In this context, we analyzed the pathogenicity of Metarhizium anisopliae (Metschn.) strains IBCB 384 and IBCB 425 and Beauveria bassiana (Bals.-Criv.) Vuill. strains E 1764 and E 3158 against Galleria mellonella (Linn.) larvae, during pre-invasion and post-invasion phases. The results showed M. anisopliae, especially strain IBCB 384, was most virulent in the pre-invasion phase against G. mellonella, whereas B. bassiana, especially strain E 1764, was most virulent in the post-invasion phase. During in vivo development and in the production of toxic serum, B. bassiana E 3158 was the most virulent. Different fungal growth (or toxin) strategies were observed for studied strains. Metarhizium anisopliae IBCB 425 prioritizes the growth strategy, whereas strain IBCB 384 and B. bassiana strains E 1764 and E 3158 have a toxic strategy. All strains have pathogenicity against G. mellonella, indicating their possible use for biocontrol.

Heat-stressed Metarhizium anisopliae: viability (in vitro) and virulence (in vivo) assessments against the tick Rhipicephalus sanguineus

2016 ◽  
Vol 116 (1) ◽  
pp. 111-121 ◽  
Author(s):  
Fabrício M. Alves ◽  
Cíntia C. Bernardo ◽  
Flávia R. S. Paixão ◽  
Lucas P. Barreto ◽  
Christian Luz ◽  
...  
Keyword(s):  
Metarhizium Anisopliae ◽  
Rhipicephalus Sanguineus

scholarly journals Interactions of Destruxin A with Silkworms’ Arginine tRNA Synthetase and Lamin-C Proteins

Toxins ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 137
Author(s):  
Jingjing Wang ◽  
Qunfang Weng ◽  
Fei Yin ◽  
Qiongbo Hu
Keyword(s):  
Metarhizium Anisopliae ◽  
Trna Synthetase ◽  
Regulation Of Transcription ◽  
Tail Domain ◽  
Docking Model ◽  
C Proteins ◽  
Cellular Thermal Shift Assay ◽  
Thermal Shift

Destruxin A (DA), a cyclodepsipeptidic mycotoxin produced by entomopathogenic fungus Metarhizium anisopliae, has good insecticidal activity and potential to be a new pesticide. However, the mechanism of action is still obscure. Our previous experiments showed that DA was involved in regulation of transcription and protein synthesis and suggested that silkworms’ arginine tRNA synthetase (BmArgRS), Lamin-C Proteins (BmLamin-C) and ATP-dependent RNA helicase PRP1 (BmPRP1) were candidates of DA-binding proteins. In this study, we employed bio-layer interferometry (BLI), circular dichroism (CD), cellular thermal shift assay (CETSA), and other technologies to verify the interaction of DA with above three proteins in vitro and in vivo. The results of BLI indicated that BmArgRS and BmLamin-C were binding-protein of DA with KD value 5.53 × 10−5 and 8.64 × 10−5 M, but not BmPRP1. These interactions were also verified by CD and CETSA tests. In addition, docking model and mutants assay in vitro showed that BmArgRS interacts with DA at the pocket including Lys228, His231, Asp434 and Gln437 in its enzyme active catalysis region, while BmLamin-C binds to DA at His524 and Lys528 in the tail domain. This study might provide new insight and evidence in illustrating molecular mechanism of DA in breaking insect.

Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

1974 ◽  
Vol 32 ◽  
pp. 54-55
Author(s):  
S. Phyllis Steamer ◽  
Rosemarie L. Devine
Keyword(s):  
Structural Changes ◽  
Radiation Treatment ◽  
Arterial Stenosis ◽  
Ultrastructural Changes ◽  
Vascular Effects ◽  
Microvascular Changes ◽  
Surrounding Connective Tissue ◽  
Fission Neutrons ◽  
Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

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