scholarly journals Plant regeneration of Aloysia citrolodora L. from shoot tip explants

2020 ◽  
Keyword(s):  
Plant Regeneration ◽  
Shoot Tip ◽  
Shoot Tip Explants

In Vitro direct plant regeneration using shoot tip explants in sugarcane (Saccharum officinarum L.) for rapid mass cloning

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Rupinder Kaur ◽  
Manish Kapoor
Keyword(s):  
Plant Regeneration ◽  
Saccharum Officinarum ◽  
Shoot Tip ◽  
Direct Plant Regeneration ◽  
Shoot Tip Explants ◽  
Rapid Mass

In Vitro direct plant regeneration using shoot tip explants in sugarcane (Saccharum officinarum L.) for rapid mass cloning

scholarly journals In vitro Plant Regeneration from Shoot tip Explants of Exacum travancoricum Beedi

1970 ◽  
Vol 20 (2) ◽  
pp. 113-118 ◽  
Author(s):  
B. Janarthanam ◽  
E. Sumathi Sumathi
Keyword(s):  
Plant Regeneration ◽  
Growth Response ◽  
Average Length ◽  
Shoot Tip ◽  
Average Height ◽  
In Vitro Plant Regeneration ◽  
Farm Yard Manure ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants of Exacum travancoricum, an endangered herb mainly known for ornamental purpose grown on MS with 4.44 μM BAP and 1.34 μM NAA showed better growth response and produced 29.3 ± 0.3 shoots per explant with an average length of 4.6 ± 0.1 cm after 35 days. Roots were induced after transfer to half strength of MS supplemented with 2.46 μM IBA produced 4.8 ± 0.62 roots with an average height of 3.6 ± 0.10 cm after 30 days. The rooted plantlets were transferred for hardening, 80 per cent of plantlets survived and resumed growth in the mixture of soil, vermiculite and farm yard manure (1 : 1 : 1). Key words: Exacum travancoricum; Shoot tip; Plant regeneration D.O.I. 10.3329/ptcb.v20i2.6889 Plant Tissue Cult. & Biotech. 20(2): 113-118, 2010 (December)

scholarly journals In vitro propagation and cytological analysis of Sophora mollis Royle: an endangered medicinal shrub

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aakriti Bhandari ◽  
Harminder Singh ◽  
Amber Srivastava ◽  
Puneet Kumar ◽  
G. S. Panwar ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Average Length ◽  
Germplasm Conservation ◽  
Basic Chromosome Number ◽  
Shoot Tip ◽  
Ex Situ ◽  
Ms Medium ◽  
Basic Chromosome ◽  
Shoot Tip Explants

Abstract Background Sophora mollis Royle (family Fabaceae, subfamily-Papilionaceae) is a multipurpose legume distributed in plains and foothills of the North-West Himalaya to Nepal and is facing high risk of extinction due to habitat loss and exploitation by the local people for its fuel and fodder values. Therefore, the present study was conducted to standardize a micropropagation protocol for Sophora mollis by using shoot tip explants and to study the meiotic chromosome count in the species. Results Multiple shoots were induced in shoot tip explants of Sophora mollis in Murashige and Skoog medium supplemented with different concentrations of cytokinins alone (BAP, TDZ, and Kinetin) and in combination with varying concentrations of NAA. MS medium supplemented with BAP (8.9 μM) was observed to be the optimal medium for multiple shoot induction and maximum 25.32 shoots per explant was obtained with average length of 4.5 ± 0.8 cm. In vitro developed shoots were transferred onto rooting media supplemented with different concentrations of auxin (IAA, IBA, and NAA). Maximum 86% rooting was observed in half-strength MS medium supplemented with 21.20 μM NAA with an average of 21.26 roots per culture. In vitro raised plantlets were adapted to greenhouse for better acclimatization and 60% plants were successfully transferred to the open environment. Based on the chromosome counts available from the literature and the current study, the species tend to show a basic chromosome number of x = 9. Conclusion The micropropagation protocol standardized can be helpful for the ex situ mass multiplication and germplasm conservation of the endangered species. Moreover, the ex situ conservation approach will be helpful in actively bridging the gap between ex situ and in situ approaches through the reintroduction of species in the wild. The cytological studies revealed the basic chromosome number x = 9 of the species.

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals In vitro shoot proliferation of Passiflora caerulea L. via cotyledonary node and shoot tip explants

2017 ◽  
Vol 2 ◽  
pp. 113-119 ◽  
Author(s):  
Marziyeh Jafari ◽  
Mohammad Hosein Daneshvar ◽  
Amin Lotfi
Keyword(s):  
Shoot Proliferation ◽  
Cotyledonary Node ◽  
Shoot Tip ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Proliferation
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