PECULIARITIES OF THE EFFECTS OF BILE ACIDS ON ATPASE ACTIVITY OF THE COLON MUCOSA IN PATIENTS WITH OVERWEIGHT AND IRRITABLE BOWEL SYNDROME

The aim is to investigate the effect of bile acids on the ATPase activity of the colon mucosa in patients with overweight and irritable bowel syndrome (IBS). Materials and methods: Completely examined 12 patients with IBS and overweight.We estimated the ATPase activity of colon mucous of the patients with IBSspectrophotometrically by determined the content of orthophosphate that was released after ATP hydrolysis. We studied the effect of 3-sulphate of taurolitocholate (TLC-S) on specific activities of Na+/ K+-ATPase, Ca2+-ATPase of endoplasmatic reticulum (EPR),Ca2+-ATPase of plasmatic membrane (PM) and basal Mg2+-ATPase of postmitochondrial subcellular fraction of colon mucous of the patients with IBS. Results: We establishedthe specific activities of Na+/K+-ATPase, Ca2+-ATPase of EPR,Ca2+-ATPase of PM and basal Mg2+-ATPase. Therewere(6.06 ± 1.61), (5.88 ± 1.19), (8.86 ± 1.56) (6.44 ± 2.02)μmol Pi/ mg protein per hour, respectively. TLC-S (50 μM) did not causedany change of Na+/K+-ATPase , as well as Ca2+-ATPasesactivities, but statistically significant increased activity of Mg2+-ATPase of postmitochondrial subcellular fraction of colon mucous of the patients with IBS by 4 fold. Conclusions: TLC-S increased basal Mg2+-ATPase in the postmitochondrial fraction of colon mucous of the patients with overweight and IBS, but had no effect on Na+/K+-ATPase and Ca2+-ATPases activities. It has been suggested that activation of basal Mg2+-ATPase under by TLC-S may indicates the role of the endo-lysosomal system of epitheliocytes of colon mucous in developing of pathology IBS.

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IDDF2019-ABS-0182 A clostridia-rich enterotype contributes to increased excretion of bile acids in diarrhea-predominant irritable bowel syndrome

10.1136/gutjnl-2019-iddfabstracts.34 ◽

2019 ◽

Author(s):

Ling Zhao

Keyword(s):

Irritable Bowel Syndrome ◽

Bile Acids ◽

Irritable Bowel

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Inflammatory cell distribution in colon mucosa as a new tool for diagnosis of irritable bowel syndrome: A promising pilot study

Neurogastroenterology & Motility ◽

10.1111/nmo.13223 ◽

2017 ◽

Vol 30 (1) ◽

pp. e13223 ◽

Cited By ~ 11

Author(s):

J. Boyer ◽

M.-C. Saint-Paul ◽

B. Dadone ◽

S. Patouraux ◽

M.-H. Vivinus ◽

...

Keyword(s):

Irritable Bowel Syndrome ◽

Pilot Study ◽

Inflammatory Cell ◽

Cell Distribution ◽

Colon Mucosa ◽

Irritable Bowel

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Effect of colesevelam on faecal bile acids and bowel functions in diarrhoea-predominant irritable bowel syndrome

Alimentary Pharmacology & Therapeutics ◽

10.1111/apt.13065 ◽

2015 ◽

Vol 41 (5) ◽

pp. 438-448 ◽

Cited By ~ 58

Author(s):

M. Camilleri ◽

A. Acosta ◽

I. Busciglio ◽

A. Boldingh ◽

R. B. Dyer ◽

...

Keyword(s):

Irritable Bowel Syndrome ◽

Bile Acids ◽

Irritable Bowel

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Fecal Bile Acids and Fecal Short Chain Fatty Acids in Patients with Irritable Bowel Syndrome and Control Volunteers

Proceedings of IMPRS ◽

10.18060/22742 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

Chirag Patel ◽

Carolyn Lockett ◽

Huiping Xu ◽

Andrea Shin

Keyword(s):

Fatty Acids ◽

Irritable Bowel Syndrome ◽

Organic Acids ◽

Bile Acids ◽

Short Chain Fatty Acids ◽

Small Sample ◽

Short Chain ◽

Fecal Bile Acids ◽

Irritable Bowel ◽

Chain Fatty Acids

Background and Aims: Fecal bile acids (BAs), short chain fatty acids (SCFAs), and gut microbiome may be implicated in irritable bowel syndrome (IBS) pathophysiology. Our aim was to compare fecal organic acids between IBS with constipation (IBS-C), IBS with diarrhea (IBS-D), and controls. Methods: Stool samples were collected from 17 controls, 5 IBS-C, and 5 IBS-D volunteers during a 4-day high fat diet. Aliquots were stored for future analysis of the fecal microbiota. Fecal SCFA and BA analyses were conducted at the Metabolite Profiling Facility at Purdue University and Laboratory Medicine and Pathology at Mayo Clinic. We compared SCFA and BA levels among groups using the Wilcoxon rank sum test. Gamma and linear regression were used to compare SCFAs and BAs adjusting for age and body mass index (BMI). Results: Fecal acetate levels (mean+SD, µg/mg) were higher in IBS-C (11.3±7) than in controls (6.1±3.3) or IBS-D (7.7±2), although not statistically significant (p=0.19). Total fecal BAs (median [IQR], %) were higher in IBS-D (675 [484-778]) than in controls (342 [130-640]) or IBS-C (321.5 (34.5-718); however, differences were not significant. No significant differences were observed in BAs or SCFAs between groups in multivariate analyses. Conclusion: We are unable to show significant differences in organic acid levels in IBS and controls. Lack of association may be due to small sample size. Future investigation of larger patient numbers with incorporation of transit and microbiome analyses may shed further light on the role of organic acids in IBS to identify new biomarkers and treatment targets.

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