scholarly journals Usefulness of MALDI-TOF and REP-PCR against PFGE for the epidemiological study of Acinetobacter baumannii

2021 ◽  
Author(s):  
Cristina García-Salguero ◽  
◽  
Esther Culebras ◽  
Adela Alvarez-Buylla ◽  
Icíar Rodríguez-Avial ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Epidemiological Study ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pcr Analysis ◽  
Maldi Tof Ms ◽  
Clonal Group ◽  
Maldi Tof ◽  
Typing Methods ◽  
Tof Ms

Objective. To evaluate the ability of MALDI-TOF MS and rep-PCR to discriminate Acinetobacter baumannii clones. Material and methods. A total of 21 strains of A. baumannii with different epidemiological and phenotipycal characteristics were included in the study. All isolates were analyzed in parallel by MALDI-TOF MS and rep-PCR and the spectra obtained were compared with each other and with the results obtained by pulsed field gel electrophoresis (PFGE). Isolates with a similarity equal to or greater than 87% were considered to be part of the same clonal group. Results. The analysis of the 21 isolates included in the study, resulted in 8 clonal groups using PFGE, 3 groups by MALDI-TOF MS and 7 groups by rep-PCR analysis. The isolates that formed the different groups by the 3 techniques used were totally different, so it can be concluded that there is no equivalence between the results obtained with the three typing methods used. Conclusions. Despite its simplicity, neither MALDI-TOF MS nor rep-PCR can at this time replace PFGE for the epidemiological study of A. baumannii.

scholarly journals Rapid Classification of Clostridioides difficile Strains Using MALDI-TOF MS Peak-Based Assay in Comparison with PCR-Ribotyping

2021 ◽  
Vol 9 (3) ◽  
pp. 661
Author(s):  
Adriana Calderaro ◽  
Mirko Buttrini ◽  
Monica Martinelli ◽  
Benedetta Farina ◽  
Tiziano Moro ◽  
...  
Keyword(s):  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Clostridioides Difficile ◽  
Pcr Ribotyping ◽  
Typing Methods ◽  
Set Up ◽  
Tof Ms

Typing methods are needed for epidemiological tracking of new emerging and hypervirulent strains because of the growing incidence, severity and mortality of Clostridioides difficile infections (CDI). The aim of this study was the evaluation of a typing Matrix-Assisted Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS (T-MALDI)) method for the rapid classification of the circulating C. difficile strains in comparison with polymerase chain reaction (PCR)-ribotyping results. Among 95 C. difficile strains, 10 ribotypes (PR1–PR10) were identified by PCR-ribotyping. In particular, 93.7% of the isolates (89/95) were grouped in five ribotypes (PR1–PR5). For T-MALDI, two classifying algorithm models (CAM) were tested: the first CAM involved all 10 ribotypes whereas the second one only the PR1–PR5 ribotypes. Better performance was obtained using the second CAM: recognition capability of 100%, cross-validation of 96.6% and agreement of 98.4% (60 correctly typed strains, limited to PR1–PR5 classification, out of 61 examined strains) with PCR-ribotyping results. T-MALDI seems to represent an alternative to PCR-ribotyping in terms of reproducibility, set up time and costs, as well as a useful tool in epidemiological investigation for the detection of C. difficile clusters (either among CAM included ribotypes or out-of-CAM ribotypes) involved in outbreaks.

scholarly journals Exploiting intrinsic fluorescence spectroscopy to discriminate between Acinetobacter calcoaceticus–Acinetobacter baumannii complex species

RSC Advances ◽  
2017 ◽  
Vol 7 (14) ◽  
pp. 8581-8588 ◽  
Author(s):  
Ana Sofia Silva ◽  
Cristina Quintelas ◽  
Eugénio C. Ferreira ◽  
João A. Lopes ◽  
Clara Sousa
Keyword(s):  
Fluorescence Spectroscopy ◽  
Acinetobacter Baumannii ◽  
Acinetobacter Calcoaceticus ◽  
Intrinsic Fluorescence ◽  
Bacterial Typing ◽  
Complex Species ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Acinetobacter Baumannii Complex ◽  
Tof Ms

Spectroscopy for bacterial typing purposes. Instrisinc fluorescence versus FTIR-ATR and MALDI-TOF MS.

scholarly journals Unsuitability of MALDI-TOF MS to discriminate Acinetobacter baumannii clones under routine experimental conditions

2015 ◽  
Vol 6 ◽  
Author(s):  
Clara Sousa ◽  
João Botelho ◽  
Filipa Grosso ◽  
Liliana Silva ◽  
João Lopes ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Experimental Conditions ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Xác định vi khuẩn Acinetobacter calcoaceticus - Acinetobacter baumannii complex kháng kháng sinh carbapenem phân lập trong rau ăn sống và thịt chế biến sẵn trên địa bàn thành phố Hà Nội

2021 ◽  
Vol 4 (1) ◽  
pp. 3-5
Author(s):  
Yến Tạ Thị ◽  
Loan Phạm Thị ◽  
Hạnh Ninh Thị ◽  
Hải Hà Vũ Thị ◽  
Quân Phạm Văn ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Acinetobacter Calcoaceticus ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Acinetobacter Baumannii Complex ◽  
Acinetobacter Spp ◽  
Tof Ms

Acinetobacter spp. là vi khuẩn gây bệnh phổ biến ở người. Chi Acinetobacter bao gồm khoảng 65 loài, trong đó nhóm Acinetobacter calcoaceticus - Acinetobacter baumannii complex được xác định là nguyên nhân gây ra hơn 80% trường hợp nhiễm trùng tại bệnh viện ở bệnh nhân suy giảm miễn dịch. Các phân tích kiểu hình hiện có không đủ để xác định chính xác và phân biệt các loài Acinetobacter spp. quan trọng về mặt lâm sàng. Trong tổng số 480 mẫu rau ăn sống và thịt chế biến sẵn được thu thập tại 6 quận nội thành Hà Nội gồm Đống Đa, Hoàng Mai, Hai Bà Trưng, Long Biên, Hà Đông, Cầu Giấy, có 288 mẫu (chiếm 60%) mẫu nhiễm Acinetobacter spp. Kết quả  định danh bằng kỹ thuật MALDI-TOF MS ghi nhận 208 (43%, n = 480) mẫu nhiễm Acinetobacter calcoaceticus-Acinetobacter baumannii complex, trong đó 156 (65%, n = 240) mẫu rau ăn sống và 52 (22%, n = 240) mẫu thịt nhiễm Acinetobacter calcoaceticus-Acinetobacter baumannii complex. Thử nghiệm kháng sinh đồ bằng các khoanh giấy kháng sinh cho thấy 82% số chủng vi khuẩn Acinetobacter calcoaceticus-Acinetobacter baumannii complex kháng với kháng sinh imipenem và 30% số chủng kháng với meropenem.

scholarly journals Acinetobacter baumannii Kan İzolatlarının MALDI-TOF MS, ARDRA ve blaOXA-51-benzeri Gene Özgül Gerçek Zamanlı Polimeraz Zincir Reaksiyonu ile Tanımlanması

2020 ◽  
Vol 54 (4) ◽  
pp. 535-546
Author(s):  
Ayşegül Gözalan ◽  
Sibel Aydoğan ◽  
Demet Hacıseyitoğlu ◽  
Çiğdem Kuzucu ◽  
Fatma Köksal ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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