A simple, rapid and high-resolution banding method in polyacrylamide gels

2008 ◽  
Vol 30 (2) ◽  
pp. 251-254 ◽  
Author(s):  
Yu-Shan ZHANG
Keyword(s):  
High Resolution ◽  
Polyacrylamide Gels ◽  
Resolution Banding

Cloning of trg, a gene for a sensory transducer in Escherichia coli

1982 ◽  
Vol 152 (1) ◽  
pp. 372-383
Author(s):  
S Harayama ◽  
P Engström ◽  
H Wolf-Watz ◽  
T Iino ◽  
G L Hazelbauer
Keyword(s):  
Escherichia Coli ◽  
High Resolution ◽  
Sodium Dodecyl Sulfate ◽  
Structural Gene ◽  
Preliminary Analysis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gels ◽  
Physiological Functions ◽  
The Common ◽  
Dependent Modification

Clones of trg, a gene which codes for a chemotactic transducer, were isolated linked to ColE1 and pBR322 vectors. Studies with the hybrid plasmids demonstrated unequivocally that trg is the structural gene for methyl-accepting chemotaxis protein III. The Trg protein was found to be structurally complex, electrophoresing as a series of seven bands on high-resolution sodium dodecyl sulfate-polyacrylamide gels. The multiplicity of bands is a function of the activity of cheR, which codes for a methyltransferase, and of cheB, which codes for a demethylase. It appears that Trg, a quantitatively minor transducer, resembles the two major transducer proteins, Tsr and Tar, in that all three are multiply methylated and also multiply modified in a second way which requires an active cheB gene. However, preliminary analysis of the Trg protein indicated that it is significantly less related structurally to the Tsr or Tar protein than those two transducers are to each other. This implies that the features of multiple methylation and cheB-dependent modification are likely to be critical for the common physiological functions in chemotactic excitation and adaptation performed by all three transducers.

High resolution NMR imaging: Gd-DTPA labeled enzyme as a probe for permeability studies in polyacrylamide gels

1991 ◽  
Vol 9 (4) ◽  
pp. 583-587 ◽  
Author(s):  
M. Spanoghe ◽  
D. Lanens ◽  
C. Gorrebeeck ◽  
R. Dommisse ◽  
G. Lemière ◽  
...  
Keyword(s):  
High Resolution ◽  
Nmr Imaging ◽  
Polyacrylamide Gels ◽  
High Resolution Nmr ◽  
Permeability Studies
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