resolution method
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2022 ◽  
Vol 72 ◽  
pp. 103372
Li Kang ◽  
Guojuan Liu ◽  
Jianjun Huang ◽  
Jianping Li

2021 ◽  
Wenda Yang ◽  
Minggong Wu ◽  
Xiangxi Wen ◽  
Kexin Bi ◽  
Fugen Lin

2021 ◽  
Courtney C Johnson ◽  
Jack Exell ◽  
Yuxin Lin ◽  
Jonathan Aguilar ◽  
Kevin Welsher

The early stages of the virus-cell interaction have long evaded observation by existing microscopy methods due to the rapid diffusion of virions in the extracellular space and the large 3D cellular structures involved. Here we present an active-feedback single-virus tracking method with simultaneous volumetric imaging of the live cell environment to address this knowledge gap to present unprecedented detail to the extracellular phase of the infectious cycle. We report previously unobserved phenomena in the early stages of the virus-cell interaction, including skimming contact events at the millisecond timescale, orders of magnitude change in diffusion coefficient upon binding, and cylindrical and linear diffusion modes along filopodia. Finally, we demonstrate how this new method can move single-virus tracking from simple monolayer culture towards more tissue-like conditions by tracking single virions in tightly packed epithelial cells. This multi-resolution method presents new opportunities for capturing fast, 3D processes in biological systems.

2021 ◽  
Vol 13 (24) ◽  
pp. 5118
Xiaowan Li ◽  
Fubo Zhang ◽  
Yanlei Li ◽  
Qichang Guo ◽  
Yangliang Wan ◽  

Tomographic Synthetic Aperture Radar (TomoSAR) is a breakthrough of the traditional SAR, which has the three-dimentional (3D) observation ability of layover scenes such as buildings and high mountains. As an advanced system, the airborne array TomoSAR can effectively avoid temporal de-correlation caused by long revisit time, which has great application in high-precision mountain surveying and mapping. The 3D reconstruction using TomoSAR has mainly focused on low targets, while there are few literatures on 3D mountain reconstruction. Due to the layover phenomenon, surveying in high mountain areas remains a difficult task. Consequently, it is meaningful to carry out the research on 3D mountain reconstruction using the airborne array TomoSAR. However, the original TomoSAR mountain point cloud faces the problem of elevation ambiguity. Furthermore, for mountains with complex terrain, the points located in different elevation periods may intersect. This phenomenon increases the difficulty of solving the problem. In this paper, a novel elevation ambiguity resolution method is proposed. First, Density-Based Spatial Clustering of Applications with Noise (DBSCAN) and Gaussian Mixture Model (GMM) are combined for point cloud segmentation. The former ensures coarse segmentation based on density, and the latter allows fine segmentation of the abnormal categories caused by intersection. Subsequently, the segmentation results are reorganized in the elevation direction to reconstruct all possible point clouds. Finally, the real point cloud can be extracted automatically under the constraints of the boundary and elevation continuity. The performance of the proposed method is demonstrated by simulations and experiments. Based on the airborne array TomoSAR experiment in Leshan City, Sichuan Province, China in 2019, the 3D model of the surveyed mountain is presented. Moreover, three kinds of external data are applied to fully verify the validity of this method.

2021 ◽  
Tong Zhou ◽  
Ziyue Tang ◽  
yichang chen ◽  
yongjian sun

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