Linkage Relationship between T-type Restorer Gene Rf3 and K-type Male Sterile Gene rfv1 in Triticum macha L.

2009 ◽  
Vol 34 (10) ◽  
pp. 1757-1761 ◽  
Author(s):  
Xi-Yue SONG
Keyword(s):  
Restorer Gene ◽  
Linkage Relationship ◽  
Male Sterile ◽  
Triticum Macha

scholarly journals Glucose-6-phosphate Isomerase as a Marker of a Fertility Restorer Gene in Rape – Short Communication

2011 ◽  
Vol 39 (No. 4) ◽  
pp. 130-133 ◽  
Author(s):  
J. Horáček ◽  
M. Ačanová
Keyword(s):  
Short Communication ◽  
Polyacrylamide Gel Electrophoresis ◽  
Restorer Gene ◽  
Male Sterile ◽  
Flowering Stage ◽  
Fertility Restorer ◽  
Brassica Napus L ◽  
Native Page ◽  
Isozyme Markers ◽  
Early Stages

 In breeding of winter rape (Brassica napus L. var. napus) using the OGU-INRA system, based on cytoplasmic male sterility (CMS), it is necessary to distinguish pollen-sterile lines, carrying CMS factors and the recessive fertility restorer alleles rfrf, from fertile lines, carrying at least one dominant restorer gene allele (Rfrf or RfRf). To grow plants till the flowering stage takes much time. The method was therefore modified using isozyme markers of glucose-6-phosphate isomerase (PGI) to distinguish male sterile (MS) from male-fertile lines in early stages. Since the restorer gene is tightly linked to the markers and the PGI isozymes can be distinguished by electrophoresis, the markers can be used to identify MS rape plants in early stages. Also, homozygous and heterozygous fertility-restored plants can be separated this way. In our work we tried to optimise the distinction of pollen-fertile and pollen-sterile rape plants with PGI isozyme markers, using vertical polyacrylamide gel electrophoresis (native-PAGE). The method will be used for the breeding of rapeseed with the OGU-INRA system.  

scholarly journals CYTOPLASMIC MALE STERILITY IN BARLEY. XI. THE msm2 CYTOPLASM

Genetics ◽  
1982 ◽  
Vol 102 (2) ◽  
pp. 285-295
Author(s):  
H Ahokas
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Male Fertility ◽  
Fertility Restoration ◽  
Nuclear Gene ◽  
Restorer Gene ◽  
Male Sterile ◽  
Partial Restoration ◽  
Restoration Ability ◽  
Selection Of

ABSTRACT A new cytoplasmic male sterility in barley (Hordeum vulgare s.l.) is described and designated as msm2. The cytoplasm was derived from a selection of the wild progenitor of barley (H. vulgare ssp. spontaneum). This selection, 79BS14-3, originates from the Southern Coastal Plain of Israel. The selection 79BS14-3 has a normal spike fertility in Finland. When 79BS14-3 was crossed by cv. Adorra, the F1 displayed partial male fertility and progeny of recurrent backcrosses with cv. Adorra were completely male sterile. Evidently 79BS14-3 is a carrier of a recessive or semidominant restorer gene of fertility. The dominant restorer gene Rfm1a for another cytoplasmic male sterility, msm1, is also effective in msm2 cytoplasm. The different partial fertility restoration properties of msm2 and msm1 cause these cytoplasms to be regarded as being distinct. Seventy spontaneum accessions from Israel have been studied for their capacity to produce F1 restoration of male fertility both in msm1 and in msm2 cytoplasms with a cv. Adorra-like seed parent (nuclear gene) background. The msm2 cytoplasm shows partial restoration more commonly than msm1 in these F1 combinations. The mean restoration percentage per accession for msm2 is 28, and for msm1 4. Most of the F1 seed set differences of the two cytoplasms are statistically significant. When estimated with partially restored F1 combinations, msm2 cytoplasm appeared to be about 50 times more sensitive to the male fertility-promoting genes present in the spontaneum accessions. The spontaneum sample from Central and Western Negev, which has been found to be devoid of restoration ability in msm1 cytoplasm, had only low partial restoration ability in msm2 (mean 0.3%). The female fertility of msm2 appears normal. The new msm2 cytoplasm could be useful in producing hybrid barley.

scholarly journals Genetics of male sterility in gynodioecious Plantago coronopus. II. Nuclear genetic variation.

Genetics ◽  
1995 ◽  
Vol 139 (4) ◽  
pp. 1759-1775 ◽  
Author(s):  
H P Koelewijn ◽  
J M Van Damme
Keyword(s):  
Male Sterility ◽  
Gene Action ◽  
Restorer Gene ◽  
Consistent Difference ◽  
Male Sterile ◽  
Restorer Genes ◽  
Cytoplasmic Type ◽  
Plantago Coronopus

Abstract Inheritance of male sterility was studied in the gynodioecious species Plantago coronopus using five plants and their descendants from an area of approximately 50 m2 from each of four locations. In each location, crosses between these five plants yielded the entire array of possible sex phenotypes. Both nuclear and cytoplasmic genes were involved. Emphasis is placed on the nuclear (restorer) genetics of two cytoplasmic types. For both types, multiple interacting nuclear genes were demonstrated. These genes carried either dominant or recessive restorer alleles. The exact number of genes involved could not be determined, because different genetic models could be proposed for each location and no common genetic solution could be given. At least five genes, three with dominant and two with recessive restorer allele action, were involved with both cytoplasmic types. Segregation patterns of partially male sterile plants suggested that they are due to incomplete dominance at restorer loci. Restorer genes interact in different ways. In most instances models with independent restorer gene action were sufficient to explain the crossing results. However, for one case we propose a model with epistatic restorer gene action. There was a consistent difference in the segregation of male sterility between plants from the two cytoplasmic types. Hermaphrodites of cytoplasmic type 2 hardly segregated male steriles, in contrast to plants with cytoplasmic type 1.

The rf2 Nuclear Restorer Gene of Male-Sterile T-Cytoplasm Maize

Science ◽  
1996 ◽  
Vol 272 (5266) ◽  
pp. 1334-1336 ◽  
Author(s):  
X. Cui ◽  
R. P. Wise ◽  
P. S. Schnable
Keyword(s):  
Restorer Gene ◽  
Male Sterile ◽  
Nuclear Restorer Gene

scholarly journals A preliminary identification of Rf*-A619, a novel restorer gene for CMS-C in maize (Zea mays L.)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2719 ◽  
Author(s):  
Liu Yongming ◽  
Zhao Zhuofan ◽  
Lu Yanli ◽  
Li Chuan ◽  
Wang Jing ◽  
...  
Keyword(s):  
Inbred Line ◽  
Fertility Restoration ◽  
Pcr Amplification ◽  
Restorer Gene ◽  
Maize Inbred Line ◽  
Male Sterile ◽  
Chi Square ◽  
Restorer Genes ◽  
Genotypic Analysis ◽  
Chi Square Test

C-type cytoplasmic male sterility (CMS-C) is widely utilized for hybrid maize seed production. However, genetic mechanisms underlying the fertility restoration are very complicated. At present, there is a divergence on the number of fertility restorer genes in maize inbred line A619 for CMS-C. To further elucidate the restoring mechanism of A619, we used genetic analysis and molecular markers to confirm the restorer genes of maize inbred line A619 for C-type male sterile line C48-2 in this study. Firstly, the fertility segregations of (C48-2 × A619)F2 populations were investigated under three environments during 2013–2015. The segregation ratio of fertile and sterile plants in the F2 population fit to 15:1 via chi-square test and this result suggested that there are two dominant restorer genes in A619 for CMS-C, i.e., Rf4 and a novel gene named Rf*-A619. Next, based on the sequence differences between Rf4 and its recessive allelic rf4, a novel dominant marker F2/R2 was developed and validated to genotyping Rf4 in the F2 population. Through genotypic analysis, we found that there were a certain amount of fertile individuals without Rf4 which accounted for 3/16 in the F2 population via chi-square test at the 0.05 level. These results provided another proof to sustain that the inbred line A619 contains one additional restorer gene for CMS-C fertility restoration except Rf4. At last, we used one SSR marker which is tightly linked with the dominant restorer gene Rf5 to analyze those fertile plants without Rf4 in the F2 population. The PCR amplification results showed that Rf*-A619 is not allelic to Rf5 but a novel restorer gene for CMS-C. These results not only provide a basis for the mapping and characterization of a novel restorer gene but also give a new insight into the mechanism of CMS-C fertility restoration.

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