scholarly journals Morphological characteristics and cytological study of anther abortion of temperature-sensitive nuclear male sterile line 160S in <italic>Brassica napus</italic>

2020 ◽  
Vol 47 (5) ◽  
pp. 983-990
Author(s):  
Xin TANG ◽  
Yuan-Yuan LI ◽  
Jun-Xing LU ◽  
Tao ZHANG
Keyword(s):  
Brassica Napus ◽  
Cytological Study ◽  
Morphological Characteristics ◽  
Temperature Sensitive ◽  
Male Sterile ◽  
Male Sterile Line

Characterization of a new temperature-sensitive male sterile line SP2S in rapeseed (Brassica napus L.)

Euphytica ◽  
2015 ◽  
Vol 206 (2) ◽  
pp. 473-485 ◽  
Author(s):  
Chengyu Yu ◽  
Yingfen Guo ◽  
Juan Ge ◽  
Yumei Hu ◽  
Jungang Dong ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Temperature Sensitive ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Brassica Napus L

Cytological Observation of Anther Development of Photoperiod/Thermo-sensitive Male Sterile Line Huiyou50S in Brassica napus

2012 ◽  
Vol 38 (3) ◽  
pp. 541 ◽  
Author(s):  
Juan GE ◽  
Ying-Fen GUO ◽  
Cheng-Yu YU ◽  
Guo-Yun ZHANG ◽  
Jun-Gang DONG ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Anther Development ◽  
Cytological Observation ◽  
Male Sterile ◽  
Male Sterile Line

Cytogenetic analysis of plants regenerated from tissue explants and mesophyll protoplasts of winter rape, Brassica napus L.

1984 ◽  
Vol 26 (6) ◽  
pp. 752-761 ◽  
Author(s):  
C. A. Newell ◽  
M. L. Rhoads ◽  
D. L. Bidney
Keyword(s):  
Brassica Napus ◽  
Chromosome Number ◽  
Germ Line ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Winter Rape ◽  
Brassica Napus L ◽  
Mesophyll Protoplasts ◽  
Regenerated Plants ◽  
Chromosome Associations

Plants were regenerated from seedling tissue explants of four lines of winter rape (Brassica napus L.) including a cytoplasmic male sterile line carrying Raphanus sativus L. cytoplasm, and from leaf mesophyll protoplasts of the cytoplasmic male sterile line. Chromosome number variability was investigated in 102 regenerated plants. Mitotic root-tip cells were scored initially; those plants exhibiting mixoploidy or an altered chromosome number were reexamined at meiosis to confirm the presence of alterations in germ line cells. Plants regenerated from seedling explants included 2n = 38 diploids (87.0%) similar to the parental line, monosomics (7.8%), trisomics (2.6%), and 2n = 76 tetraploids (1.3%). The germ line number was not resolved in one mixoploid (1.3%). Protoplast-derived regenerated plants were diploid (44%), hypodiploid (20%), and tetraploid or hypotetraploid (36%). Meiotic analysis of regenerated plants showed a range of multiple chromosome associations with no plants consistently exhibiting bivalent formation only. Chromosomal alterations other than number may have been induced by culture, but could not be substantiated since multiple chromosome associations were also frequent in control plants grown from seed.Key words: plant regeneration, protoplast regeneration, Brassica napus, cytogenetics.

Premature breakdown of tapetum associated with reverse thermo-sensitive genic male-sterile line Huiyou50S in rapeseed (Brassica napus)

2016 ◽  
Vol 38 (2) ◽  
Author(s):  
Chengyu Yu ◽  
Xianfeng Xu ◽  
Juan Ge ◽  
Yingfen Guo ◽  
Jungang Dong ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Male Sterile ◽  
Male Sterile Line

Interspecific introgression of male sterility from tetraploid oilseed Brassica napus to diploid vegetable B. rapa through hybridisation and backcrossing

2013 ◽  
Vol 64 (7) ◽  
pp. 652 ◽  
Author(s):  
Zhengjie Wan ◽  
Yuanbao Tan ◽  
Minhui Shi ◽  
Yuejin Xu ◽  
Nader Aryamanesh ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Morphological Characteristics ◽  
Female Parent ◽  
F1 Hybrids ◽  
Recurrent Parent ◽  
Male Sterile ◽  
Interspecific Introgression ◽  
C Genome ◽  
Inbreeding Line

Interspecific F1 hybrids were obtained from a cross between a male sterile Brassica napus (2n = 4x = 38, AA (20) and CC (18) genomes) and an inbreeding line B. rapa (Purple Cai-Tai inbred line 9418, 2n = 2x = 20, AA (20) genome) to introgress male sterility from a tetraploid into a diploid through backcrossing. The morphological characteristics of F1 plants were more like the female parent B. napus and segregated considerably in BC1 when backcrossed to the recurrent parent Purple Cai-Tai. The progeny became stable and more similar to Purple Cai-Tai by BC4. Most C genome chromosomes were found to be eliminated, based on cytogenetic analysis. The majority of chromosomes were eliminated at very early backcross stages, with only 20–26 chromosomes in BC1 plants, and some chromosomes were eliminated gradually with increased backcross generations. The BC4 plants were generally stable with exactly 20 chromosomes. Analysis by AFLP indicated that 49.5–68.7% of the total bands eliminated from F1 to BC4 were female parent specific, and ~12% of B. napus bands were retained with increased backcrossing. The genetic materials controlling sterility from the female parent B. napus were introgressed successfully into the BC4 plants even though most B. napus chromosomes/genetic materials were eliminated during the backcross process.

Sterility and variation resulting from the transfer of polima cytoplasmic male sterility from Brassica napus into Brassica chinensis

1997 ◽  
Vol 128 (3) ◽  
pp. 299-301 ◽  
Author(s):  
B. HU ◽  
F. CHEN ◽  
Q. LI
Keyword(s):  
Control System ◽  
Brassica Napus ◽  
Interspecific Crosses ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Brassica Chinensis ◽  
Full Flowering ◽  
Cms Line ◽  
Pollination Control

Male sterile combinations made from interspecific crosses between the polima CMS line of Brassica napus and varieties of B. chinensis were backcrossed to BC3. Twenty-six selfing lines from B. chinensis were tested for their ability to either maintain complete sterility or to restore fertility in crosses with the polima male sterile line. Results show that four of these hybrids were completely male sterile and two were fertile. The sterility of the B. chinensis with polima cytoplasm was much more stable than male sterile lines with B. campestris and B. chinensis cytoplasm, which were sterile before full flowering but progressively became fertile as flowering proceeded. The results suggest that polima cytoplasm could be a suitable male sterile-inducing allocytoplasm for B. chinensis, as both maintainers and restorers are available, and could supply a reliable pollination control system for hybrid seed production in this species.

SEED PRODUCTION OF HYBRID SUMMER RAPE IN THE FIELD USING THE POL CYTOPLASMIC MALE STERILITY SYSTEM: A FIRST ATTEMPT

1990 ◽  
Vol 70 (3) ◽  
pp. 611-618 ◽  
Author(s):  
R. PINNISCH ◽  
P. B. E. McVETTY
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Seed Production ◽  
Seed Yield ◽  
Hybrid Seed ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Brassica Napus L ◽  
Total Seed ◽  
Seed Yields

Hybrid summer rape (Brassica napus L.) seed production blocks were established at two locations in Manitoba in 1986 and 1987 to examine the effect of distance from the pollen source on seed yield, (both total and hybrid) and percent hybridity of seed produced on rows of a male sterile line of the open pollinated population B. napus cultivar, Marnoo, possessing the pol cytoplasmic male sterility inducing cytoplasm. A 10:1 ratio of male sterile line (A-line) to pollen parent was employed. Leaf cutter bees (Megachile rotundata F.) were used as the pollen vector between the two parents. In 1986, no significant differences in total seed yield were found among A-line rows, while in 1987, significant differences in total seed yields of A-line rows were found. Differences in hybrid seed yields among A-line rows were significant for all locations and years. Leaf cutter bees were found to be effective pollinators of the A-line plants. Less than half and less than a third of the seed produced on the A-line rows in 1986 and 1987, respectively, was hybrid seed. The high percentage of non-hybrid seed present in the seed lot may have been due to incomplete male sterility of the Marnoo A-line population. Total seed yields, hybrid seed yields and percent hybridity all declined linearly as distance from the pollen source increased. Improvement in the degree of male sterility of the Marnoo A-line population and/or a reduction in the 10:1 ratio of parents, and subsequent maximum A-line row to R-line row distance, will be necessary if hybrid summer rape seed production using this pol CMS A-line is to be commercially viable.Key words: Brassica napus L., CMS, hybrid, hybridity

scholarly journals Identification of a microsatellite marker linked to the fertility-restoring gene for a polima cytoplasmic male-sterile line in Brassica napus L.

2011 ◽  
Vol 10 (47) ◽  
pp. 9563-9569 ◽  
Author(s):  
Li Yun ◽  
Liu Zhi ◽  
Cai Qiang ◽  
Yang Guangsheng ◽  
He Qingbiao ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Microsatellite Marker ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Brassica Napus L ◽  
Cytoplasmic Male Sterile
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