Sterility and variation resulting from the transfer of polima cytoplasmic male sterility from Brassica napus into Brassica chinensis

1997 ◽  
Vol 128 (3) ◽  
pp. 299-301 ◽  
Author(s):  
B. HU ◽  
F. CHEN ◽  
Q. LI
Keyword(s):  
Control System ◽  
Brassica Napus ◽  
Interspecific Crosses ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Brassica Chinensis ◽  
Full Flowering ◽  
Cms Line ◽  
Pollination Control

Male sterile combinations made from interspecific crosses between the polima CMS line of Brassica napus and varieties of B. chinensis were backcrossed to BC3. Twenty-six selfing lines from B. chinensis were tested for their ability to either maintain complete sterility or to restore fertility in crosses with the polima male sterile line. Results show that four of these hybrids were completely male sterile and two were fertile. The sterility of the B. chinensis with polima cytoplasm was much more stable than male sterile lines with B. campestris and B. chinensis cytoplasm, which were sterile before full flowering but progressively became fertile as flowering proceeded. The results suggest that polima cytoplasm could be a suitable male sterile-inducing allocytoplasm for B. chinensis, as both maintainers and restorers are available, and could supply a reliable pollination control system for hybrid seed production in this species.

Characterization of a new temperature-sensitive male sterile line SP2S in rapeseed (Brassica napus L.)

Euphytica ◽  
2015 ◽  
Vol 206 (2) ◽  
pp. 473-485 ◽  
Author(s):  
Chengyu Yu ◽  
Yingfen Guo ◽  
Juan Ge ◽  
Yumei Hu ◽  
Jungang Dong ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Temperature Sensitive ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Brassica Napus L

scholarly journals Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus

2021 ◽  
Vol 12 ◽  
Author(s):  
Qian Yang ◽  
Xiaoyi Nong ◽  
Jize Xu ◽  
Fan Huang ◽  
Fang Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Bulked Segregant Analysis ◽  
Fertility Restoration ◽  
26S Proteasome ◽  
Morphological Observation ◽  
Male Sterile ◽  
Bac Clone ◽  
Cell Stage ◽  
Cms Line ◽  
Candidate Interval

Crosses that lead to heterosis have been widely used in the rapeseed (Brassica napus L.) industry. Cytoplasmic male sterility (CMS)/restorer-of-fertility (Rf) systems represent one of the most useful tools for rapeseed production. Several CMS types and their restorer lines have been identified in rapeseed, but there are few studies on the mechanisms underlying fertility restoration. Here, we performed morphological observation, map-based cloning, and transcriptomic analysis of the F2 population developed by crossing the CMS line WNJ01A with its restorer line Hui01. Paraffin-embedded sections showed that the sporogenous cell stage was the critical pollen degeneration period, with major sporogenous cells displaying loose and irregular arrangement in sterile anthers. Most mitochondrial electron transport chain (mtETC) complex genes were upregulated in fertile compared to sterile buds. Using bulked segregant analysis (BSA)-seq to analyze mixed DNA pools from sterile and fertile F2 buds, respectively, we identified a 6.25 Mb candidate interval where Rfw is located. Using map-based cloning experiments combined with bacterial artificial chromosome (BAC) clone sequencing, the candidate interval was reduced to 99.75 kb and two pentatricopeptide repeat (PPR) genes were found among 28 predicted genes in this interval. Transcriptome sequencing showed that there were 1679 DEGs (1023 upregulated and 656 downregulated) in fertile compared to sterile F2 buds. The upregulated differentially expressed genes (DEGs) were enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) lysine degradation pathway and phenylalanine metabolism, and the downregulated DEGs were enriched in cutin, suberine, and wax biosynthesis. Furthermore, 44 DEGs were involved in pollen and anther development, such as tapetum, microspores, and pollen wall development. All of them were upregulated except a few such as POE1 genes (which encode Pollen Ole e I allergen and extensin family proteins). There were 261 specifically expressed DEGs (9 and 252 in sterile and fertile buds, respectively). Regarding the fertile bud-specific upregulated DEGs, the ubiquitin–proteasome pathway was enriched. The top four hub genes in the protein–protein interaction network (BnaA09g56400D, BnaA10g18210D, BnaA10g18220D, and BnaC09g41740D) encode RAD23d proteins, which deliver ubiquitinated substrates to the 26S proteasome. These findings provide evidence on the pathways regulated by Rfw and improve our understanding of fertility restoration.

Cytological Observation of Anther Development of Photoperiod/Thermo-sensitive Male Sterile Line Huiyou50S in Brassica napus

2012 ◽  
Vol 38 (3) ◽  
pp. 541 ◽  
Author(s):  
Juan GE ◽  
Ying-Fen GUO ◽  
Cheng-Yu YU ◽  
Guo-Yun ZHANG ◽  
Jun-Gang DONG ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Anther Development ◽  
Cytological Observation ◽  
Male Sterile ◽  
Male Sterile Line

Cytogenetic analysis of plants regenerated from tissue explants and mesophyll protoplasts of winter rape, Brassica napus L.

1984 ◽  
Vol 26 (6) ◽  
pp. 752-761 ◽  
Author(s):  
C. A. Newell ◽  
M. L. Rhoads ◽  
D. L. Bidney
Keyword(s):  
Brassica Napus ◽  
Chromosome Number ◽  
Germ Line ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Winter Rape ◽  
Brassica Napus L ◽  
Mesophyll Protoplasts ◽  
Regenerated Plants ◽  
Chromosome Associations

Plants were regenerated from seedling tissue explants of four lines of winter rape (Brassica napus L.) including a cytoplasmic male sterile line carrying Raphanus sativus L. cytoplasm, and from leaf mesophyll protoplasts of the cytoplasmic male sterile line. Chromosome number variability was investigated in 102 regenerated plants. Mitotic root-tip cells were scored initially; those plants exhibiting mixoploidy or an altered chromosome number were reexamined at meiosis to confirm the presence of alterations in germ line cells. Plants regenerated from seedling explants included 2n = 38 diploids (87.0%) similar to the parental line, monosomics (7.8%), trisomics (2.6%), and 2n = 76 tetraploids (1.3%). The germ line number was not resolved in one mixoploid (1.3%). Protoplast-derived regenerated plants were diploid (44%), hypodiploid (20%), and tetraploid or hypotetraploid (36%). Meiotic analysis of regenerated plants showed a range of multiple chromosome associations with no plants consistently exhibiting bivalent formation only. Chromosomal alterations other than number may have been induced by culture, but could not be substantiated since multiple chromosome associations were also frequent in control plants grown from seed.Key words: plant regeneration, protoplast regeneration, Brassica napus, cytogenetics.

Premature breakdown of tapetum associated with reverse thermo-sensitive genic male-sterile line Huiyou50S in rapeseed (Brassica napus)

2016 ◽  
Vol 38 (2) ◽  
Author(s):  
Chengyu Yu ◽  
Xianfeng Xu ◽  
Juan Ge ◽  
Yingfen Guo ◽  
Jungang Dong ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Male Sterile ◽  
Male Sterile Line

scholarly journals Breeding of Indica glutinous cytoplasmic male sterile line WX209A via CRISPR/Cas9 mediated genomic editing

2019 ◽  
Vol 55 (No. 3) ◽  
pp. 93-100 ◽  
Author(s):  
Xin Wang ◽  
Yue Han ◽  
Xuan Feng ◽  
Yun-Zhen Li ◽  
Bao-Xiang Qin ◽  
...  
Keyword(s):  
Amylose Content ◽  
Rice Variety ◽  
High Yield ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Maintainer Line ◽  
Cytoplasmic Male Sterile ◽  
Glutinous Rice ◽  
Short Period ◽  
Cms Line

Glutinous cytoplasmic male sterile (CMS) line is necessary to select hybrid glutinous rice combination with high yield and quality. To develop glutinous CMS with low amylose content, in this study, we firstly knocked out the granule-bound starch synthase OsWaxy in 209B using CRISPR/Cas9 mediated genome editing technology and successfully obtained a glutinous maintainer line WX209B. Comparing with maintainer line 209B, WX209B showed decreased amylose contents and similar agronomic characters. And then, through one generation of hybridization and two generations of backcrossing with WX209B as the male parent and 209A as the female parent, the glutinous CMS line WX209A was successfully achieved. Our study provides a strategy to efficiently breed for the glutinous cytoplasmic male sterile line by combining CRISPR/Cas9-mediated gene editing technology with conventional backcross breeding method in a short period, which prepares the ground for further breeding of hybrid glutinous rice variety.  

Alfalfa leafcutting bee (Hymenoptera: Megachilidae) pollination of oilseed rape (Brassica napus L.) under isolation tents for hybrid seed production

2001 ◽  
Vol 81 (1) ◽  
pp. 199-204 ◽  
Author(s):  
J. J. Soroka ◽  
D. W. Goerzen ◽  
K. C. Falk ◽  
K. E. Bett
Keyword(s):  
Brassica Napus ◽  
Seed Production ◽  
Oilseed Rape ◽  
Hybrid Seed ◽  
Hybrid Seed Production ◽  
Stocking Rate ◽  
Megachile Rotundata ◽  
Male Sterile ◽  
Brassica Napus L ◽  
Stocking Rates

In this 3-yr field study, the activities of alfalfa leafcutting bees (Megachile rotundata Fabricius) (LCB) at varying stocking levels were monitored in shade cloth isolation tents containing male-sterile and male-fertile rows of oilseed rape Brassica napus L. to determine optimum conditions for hybrid seed production. Bumble bees, Bombus impatiens Cresson, were similarly tested in 1 yr; their pollination activities were minimal, and weight of seed produced on female lines in tents containing bumble bees was similar to that in tents without pollinators. Leafcutting bee activity, as measured by the number of female bees at the entrance to tunnels in the hive, at first increased, then decreased with increasing stocking rate. Duration of time spent visiting flowers did not vary with stocking rate, but was longer on male-fertile than on male-sterile flowers. Production of hybrid seed within tents varied with leafcutting bee stocking rate, with the highest seed yield achieved at stocking rates equivalent to three charges at weekly intervals of 400 000 leafcutting bees per hectare. At optimum LCB stocking rates, the method described provided sufficient seed quantity for small-plot multi-location field evaluation of oilseed rape hybrids. Key words: Megachile rotundata, alfalfa leafcutting bee, Bombus impatiens, Brassica napus, oilseed rape, hybrid seed production, isolation tents

SEED PRODUCTION OF HYBRID SUMMER RAPE IN THE FIELD USING THE POL CYTOPLASMIC MALE STERILITY SYSTEM: A FIRST ATTEMPT

1990 ◽  
Vol 70 (3) ◽  
pp. 611-618 ◽  
Author(s):  
R. PINNISCH ◽  
P. B. E. McVETTY
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Seed Production ◽  
Seed Yield ◽  
Hybrid Seed ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Brassica Napus L ◽  
Total Seed ◽  
Seed Yields

Hybrid summer rape (Brassica napus L.) seed production blocks were established at two locations in Manitoba in 1986 and 1987 to examine the effect of distance from the pollen source on seed yield, (both total and hybrid) and percent hybridity of seed produced on rows of a male sterile line of the open pollinated population B. napus cultivar, Marnoo, possessing the pol cytoplasmic male sterility inducing cytoplasm. A 10:1 ratio of male sterile line (A-line) to pollen parent was employed. Leaf cutter bees (Megachile rotundata F.) were used as the pollen vector between the two parents. In 1986, no significant differences in total seed yield were found among A-line rows, while in 1987, significant differences in total seed yields of A-line rows were found. Differences in hybrid seed yields among A-line rows were significant for all locations and years. Leaf cutter bees were found to be effective pollinators of the A-line plants. Less than half and less than a third of the seed produced on the A-line rows in 1986 and 1987, respectively, was hybrid seed. The high percentage of non-hybrid seed present in the seed lot may have been due to incomplete male sterility of the Marnoo A-line population. Total seed yields, hybrid seed yields and percent hybridity all declined linearly as distance from the pollen source increased. Improvement in the degree of male sterility of the Marnoo A-line population and/or a reduction in the 10:1 ratio of parents, and subsequent maximum A-line row to R-line row distance, will be necessary if hybrid summer rape seed production using this pol CMS A-line is to be commercially viable.Key words: Brassica napus L., CMS, hybrid, hybridity

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