Protective effect of lycopene against diabetic retinopathy in rats and the related mechanism

Objective. Hypoxia is central in the pathogenesis of diabetic retinopathy (DR). Hypoxia-inducible factor-1 (HIF-1) is the key mediator in cellular oxygen homeostasis that facilitates the adaptation to hypoxia. HIF-1 is repressed by hyperglycemia contributing by this to the development of complications in diabetes. Recent work has shown that the HIF-1A Pro582Ser polymorphism is more resistant to hyperglycemia-mediated repression, thus protecting against the development of diabetic nephropathy. In this study, we have investigated the effect of the HIF-1A Pro582Ser polymorphism on the development of DR and further dissected the mechanisms by which the polymorphism confers a relative resistance to the repressive effect of hyperglycemia. Research Design and Method. 703 patients with type 1 diabetes mellitus from one endocrine department were included in the study. The degree of retinopathy was correlated to the HIF-1A Pro582Ser polymorphism. The effect of glucose on a stable HIF-1A construct with a Pro582Ser mutation was evaluated in vitro. Results. We identified a protective effect of HIF-1A Pro582Ser against developing severe DR with a risk reduction of 95%, even when adjusting for known risk factors for DR such as diabetes duration, hyperglycemia, and hypertension. The Pro582Ser mutation does not cancel the destabilizing effect of glucose but is followed by an increased transactivation activity even in high glucose concentrations. Conclusion. The HIF-1A genetic polymorphism has a protective effect on the development of severe DR. Moreover, the relative resistance of the HIF-1A Pro582Ser polymorphism to the repressive effect of hyperglycemia is due to the transactivation activity rather than the protein stability of HIF-1α.

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Protective Effect of Angiotensin Converting Enzyme Inhibitors in Diabetic Retinopathy

Clinical Science ◽

10.1042/cs095015pa ◽

1998 ◽

Vol 95 (s39) ◽

pp. 15P-15P

Author(s):

MK Kulshrestha ◽

B Reeves ◽

R Corrall

Keyword(s):

Diabetic Retinopathy ◽

Angiotensin Converting Enzyme ◽

Protective Effect ◽

Enzyme Inhibitors ◽

Angiotensin Converting Enzyme Inhibitors ◽

Converting Enzyme ◽

Converting Enzyme Inhibitors

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The Protective Effect of the Tilted Disc Syndrome in Diabetic Retinopathy

Archives of Ophthalmology ◽

10.1001/archopht.1996.01100130224028 ◽

1996 ◽

Vol 114 (2) ◽

pp. 230 ◽

Cited By ~ 5

Author(s):

Susan M. Malinowski

Keyword(s):

Diabetic Retinopathy ◽

Protective Effect ◽

Tilted Disc Syndrome ◽

Tilted Disc

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Protective effect of simvastatin on induced diabetic retinopathy and endothelial progenitor cells in adult male albino rat, histological and immunohistochemical study

The Egyptian Journal of Anatomy ◽

10.21608/ejana.2017.5721 ◽

2017 ◽

Vol 40 (1) ◽

pp. 173-188

Author(s):

Eman Sadek ◽

Lamiaa Abd El Fattah ◽

Zeinab El Maadawi ◽

Manal Abd-El Mohsen

Keyword(s):

Diabetic Retinopathy ◽

Protective Effect ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Adult Male ◽

Immunohistochemical Study ◽

Endothelial Progenitor ◽

Albino Rat

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Exome-based case–control association study using extreme phenotype design reveals novel candidates with protective effect in diabetic retinopathy

Human Genetics ◽

10.1007/s00439-015-1624-8 ◽

2015 ◽

Vol 135 (2) ◽

pp. 193-200 ◽

Cited By ~ 25

Author(s):

Corina Shtir ◽

Mohammed A. Aldahmesh ◽

Saad Al-Dahmash ◽

Emad Abboud ◽

Hisham Alkuraya ◽

...

Keyword(s):

Diabetic Retinopathy ◽

Protective Effect ◽

Association Study ◽

Case Control ◽

Extreme Phenotype ◽

Control Association

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Protective Effect of Effective Components of Coreopsis tinctoria Nutt on Retinopathy of db/db Diabetic Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/9948609 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Li Hui ◽

Yang Tao ◽

Mao Xinmin

Keyword(s):

Diabetic Retinopathy ◽

Protective Effect ◽

Blood Sugar ◽

Mouse Models ◽

Glycosylated Hemoglobin ◽

Diabetic Mice ◽

Macroporous Resin ◽

Enrichment Method ◽

Coreopsis Tinctoria ◽

Effective Components

Objective. Diabetic retinopathy (DR) is one of the main diabetic microvascular complications in clinical practice, which features a complicated mechanism and insignificant efficacy. Therefore, it is urgent to find effective drugs. Xinjiang Coreopsis tinctoria Nutt is a rare alpine wild plant with unique effects and extremely high medicinal value. Preliminary studies have shown that it can reduce elevated blood sugar, unhealthy lipids, and antioxidants. This study was intended to investigate the protective effect of the effective components of Coreopsis tinctoria Nutt on the retinopathy of db/db diabetic mice and provide experimental basis for exploring the efficacy of Coreopsis tinctoria Nutt and the development of new drugs for the treatment of DR. Method. The db/db diabetic mouse models were used, and the effective components of Coreopsis tinctoria Nutt were obtained from Coreopsis tinctoria Nutt using macroporous resin enrichment method after alcohol extraction. These mice were divided into the normal group, model group, and high-dose Coreopsis tinctoria Nutt groups, the positive drug metformin group, and the metformin and Coreopsis tinctoria Nutt combination group. After these db/db type 2 diabetes mouse models were intervened for 10 weeks, their weight, blood sugar, glycosylated hemoglobin, serum MDA, SOD, and other indicators of each group were tested, and the expression changes of VEGF, ICAM1, PEDF, Bcl-2 in mouse retina were observed by immunohistochemistry method. Result. The effective components of Coreopsis tinctoria Nutt were obtained using macroporous resin enrichment method after alcohol extraction, which were mainly comprised of chlorogenic acid, flavone mariside, mariside, dicaffeoyl quinic acid, and flavone oxanine, with a total content of 532.82 mg/g, and the total flavonoid content of 330 mg/g. The effective components of Coreopsis tinctoria Nutt significantly reduced blood sugar and glycosylated hemoglobin and improved oxidative stress levels in db/db diabetic mice. Meanwhile, they reduced the expression of VEGF and ICAM1 in retinopathy and increased the expression of Bcl-2 and PEDF. The combination of Coreopsis tinctoria Nutt and metformin has the most significant effect. Conclusion. Coreopsis tinctoria Nutt can prevent and treat early diabetic retinopathy by affecting the expression of retinopathy-related factors.

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