PREPARATION OF COLLAGEN-SULFONATED CARBOXYMETHYL CHITOSAN/SILICONE MEMBRANE BILAYER DERMAL EQUIVALENT AND ITS APPLICATION IN THE TREATMENT OF SCALDS OF PORCINE SKIN

SummaryIncorporation into human platelets of the calcium fluorescent indicators quin2 or fura-2 at low concentrations used to measure intracellular free calcium leads to the potentiation of the effects of agonists on platelets. This was shown by increased aggregatory and secretory responses of quin2 or fura-2 loaded platelets after stimulation with ADP, PAP and with low concentrations of thrombin, collagen, the endoperoxide analog U-46619 and the calcium ionophore A 23187. Quin2 and fura-2 mediated platelet sensitisation could be due to altered arachidonic acid metabolism since it was inhibited by prior treatment with the cydooxygenase inhibitor acetylsalicylate. In contrast, platelets loaded with higher concentrations of calcium chelators exhibited diminished aggregation responses to all aggregating agents. This latter effect was accompanied by increased fluidity of the platelet plasma membrane bilayer and by the exposure of a new pool of membranes to the outer surface of platelets, as monitored with trimethylammonium- diphenylhexatriene (TMA-DPH) in platelets loaded with the non-fluorescent calcium probe analog MAPT. In contrast, low concentrations of quin2 did not potentiate shape change of platelets activated with ADP. Thus, shape change and aggregation can be influenced separately by intracellular Ca2+ chelators. We conclude that platelet responses are altered by the incorporation of intracellular calcium chelators at concentrations used to monitor intracellular calcium changes.

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Different Susceptibility of Cells of Porcine Skin and Internal Organs to Ultraviolet A–Induced Breaking of Nuclear DNA¶

Photochemistry and Photobiology ◽

10.1562/2004-08-25-ra-291.1 ◽

2005 ◽

Vol 81 (3) ◽

pp. 674 ◽

Cited By ~ 7

Author(s):

Anna Brożyna ◽

Barbara W. Chwirot

Keyword(s):

Nuclear Dna ◽

Ultraviolet A ◽

Porcine Skin ◽

Internal Organs

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Faculty Opinions recommendation of In vitro germline potential of stem cells derived from fetal porcine skin.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1019705.371774 ◽

2006 ◽

Author(s):

Kenneth Zaret

Keyword(s):

Stem Cells ◽

Porcine Skin

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Acellular porcine skin and allogeneic skin as wound-covering materials for extensive deep burns:a comparative study

Academic Journal of Second Military Medical University ◽

10.3724/sp.j.1008.2012.01207 ◽

2013 ◽

Vol 32 (11) ◽

pp. 1207-1211

Author(s):

Wu-quan LI ◽

Xiao-chen QIU ◽

Jun LIU ◽

Di-nan WEI ◽

Zong-hua CHEN ◽

...

Keyword(s):

Comparative Study ◽

Porcine Skin

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Effects of Carboxymethyl Chitosan on Yield and Whey Protein Loss in Cottage Cheese

Preventive Nutrition and Food Science ◽

10.3746/jfn.2005.10.3.231 ◽

2005 ◽

Vol 10 (3) ◽

pp. 231-238 ◽

Cited By ~ 4

Author(s):

Kyung-Tae Kim ◽

Ok-Ju Kang

Keyword(s):

Whey Protein ◽

Carboxymethyl Chitosan ◽

Cottage Cheese ◽

Protein Loss

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Carboxymethyl-hexanoyl Chitosan Nanodroplets for Ultrasonic Imaging and Drug Delivery to Tumor

Current Pharmaceutical Design ◽

10.2174/1381612824666180515122836 ◽

2018 ◽

Vol 24 (15) ◽

pp. 1682-1688 ◽

Cited By ~ 3

Author(s):

Yu Jinsui ◽

Situ Bing ◽

Luo Muhua ◽

Li Yue ◽

Liao Jianyi ◽

...

Keyword(s):

Ultrasound Imaging ◽

Tumor Therapy ◽

Carboxymethyl Chitosan ◽

Great Promise ◽

Ovarian Cancer Cells ◽

Mechanical Index ◽

Surface Charges ◽

Particle Structure ◽

Drug Release Profile ◽

Hexanoyl Chitosan

Introduction: Although a great many strategies have been proposed for tumor-targeted chemotherapy, current delivery methods of anticancer drugs present limited success with inevitable systemic toxicity. The aim of this study was to develop a new kind of theranostic carrier for targeted tumor therapy. Methods: Prior to prepare CHC-PFP-DOX, carboxymethyl-hexanoyl chitosan (CHC) was synthesized by acylation of carboxymethyl chitosan. To develop CHC-PFP, perfluoropentane (PFP), an ultrasound gas precursor, was simultaneously encapsulated into the hydrophobic inner cores of pre-formulated CHC micelle in aqueous phase via using the oil in water (O/W) emulsion method. The size distribution and surface charges of these nanodroplets were measured and the morphology was observed by transmission electron microscopy (TEM). For ultrasound imaging application, in vitro model was established to evaluate the imaging of CHC-PFP-DOX under different concentration and mechanical index. After that, the anti-tumor effect of ultrasound combined with CHC-PFPDOX on ovarian cancer cells was investigated. Results: The resulting CHC-PFP-DOX had a nano-sized particle structure, with hydrophobic anticancer DOX/PFP inner cores and a hydrophilic carboxymethyl chitosan polymer outer shell. The favorable nano-scaled size offers the potential to extravagate from veins and accumulate in tumor tissues via enhanced permeation and retention (EPR) effect. Additionally, CHC-PFP-DOX showed the ability to serve as ultrasound imaging agent at body temperature. Notably, it exhibited an ultrasound-triggered drug release profile through the external ultrasound irradiation. Further study demonstrated that ultrasound combined with CHC-PFP-DOX can improve the killing effect of chemotherapy for tumor. Conclusion: CHC-PFP-DOX holds great promise in simultaneous cancer-targeting ultrasound imaging and ultrasound- mediated delivery for cancer chemotherapy.

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Microneedle-Assisted Percutaneous Transport of Magnesium Sulfate

Current Drug Delivery ◽

10.2174/1567201817666191217093936 ◽

2020 ◽

Vol 17 (2) ◽

pp. 140-147

Author(s):

Karna B. Ghimirey ◽

Kevin Ita

Keyword(s):

Confocal Microscopy ◽

Stratum Corneum ◽

Magnesium Sulfate ◽

Inductively Coupled Plasma ◽

Optical Emission ◽

Magnesium Concentration ◽

Porcine Skin ◽

Time Curve ◽

Student’S T

Objective: In vitro diffusion experiments were performed to assess the permeation of magnesium sulfate across pig skin. Method: The mean thickness of the dermatomed porcine skin was 648 ± 12 µm. Magnesium concentration was measured using inductively coupled plasma-optical emission spectroscopy. Transdermal flux of magnesium sulfate across MN-treated and untreated porcine skin was obtained from the slope of the steady-state linear portion of cumulative amount versus time curve. Results: Statistical analysis of the results was done with Student’s t-test. The transdermal flux of magnesium sulfate across microneedle-treated porcine skin was 134.19 ± 2.4 µg/cm2/h and transdermal flux across untreated porcine skin was 4.64 ± 0.05 µg/cm2/h. Confocal microscopy was used to visualize the microchannels created by a solid microneedle roller (500 µm). Conclusion: From our confocal microscopy studies, it was evident that the 500 μm long microneedles disrupted the stratum corneum and created microchannels measuring 191 ± 37 µm. The increase in transdermal flux across the microneedle-treated skin was statistically significant compared to that of controls, i.e., without the application of microneedles. With the application of microneedles, the transdermal flux of magnesium permeated over 12 h was approximately 33-fold higher in comparison to passive diffusion across an intact stratum corneum.

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