Establishment of Real-time TaqMan-Fluorescence Quantitative RT-PCR Assay for Detection and Quantification of mRNA Expression of RAc1 of Rice

2010 ◽  
Vol 31 (1) ◽  
pp. 75-81
Author(s):  
Dong GAO ◽  
Yun-Yue WANG ◽  
Xia-Hong HE ◽  
Cheng-Yun LI ◽  
You-Yong ZHU
Keyword(s):  
Mrna Expression ◽  
Real Time ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Detection And Quantification

Detection and Quantification of Chikungunya Virus by Real-Time RT-PCR Assay

2016 ◽  
pp. 105-117 ◽  
Author(s):  
Seok Mui Wang ◽  
Ummul Haninah Ali ◽  
Shamala Devi Sekaran ◽  
Ravindran Thayan
Keyword(s):  
Real Time ◽  
Chikungunya Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Detection And Quantification

Enhancement of detection and quantification of rotavirus in stool using a modified real-time RT-PCR assay

2008 ◽  
Vol 80 (8) ◽  
pp. 1489-1496 ◽  
Author(s):  
Molly M. Freeman ◽  
Tara Kerin ◽  
Jennifer Hull ◽  
Karen McCaustland ◽  
Jon Gentsch
Keyword(s):  
Real Time ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Detection And Quantification

A Single-tube real-time RT-PCR assay for RNA detection and quantification of genetically diverse HIV including rare HIV-1 non-m groups and HIV-2

2014 ◽  
Vol 29 (4) ◽  
pp. 179-185
Author(s):  
M. A. Prasolova ◽  
M. K. Ivanov ◽  
N. M. Gashnikova ◽  
E. S. Netesova ◽  
G. M. Dymshits
Keyword(s):  
Real Time ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Rna Detection ◽  
Single Tube ◽  
Hiv 1 ◽  
Detection And Quantification

Detection and quantification of AFP mRNA in the blood of hepatocellular carcinoma patients by real-time RT-PCR assay

2000 ◽  
Vol 118 (4) ◽  
pp. A909
Author(s):  
Atsushi Miyamoto ◽  
Hiroaki Nagano ◽  
Masato Sakon ◽  
Hidetoshi Eguchi ◽  
Motoi Kondo ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Real Time ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Afp Mrna ◽  
Detection And Quantification

Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus

2007 ◽  
Vol 39 (3) ◽  
pp. 188-193 ◽  
Author(s):  
S.R. Santhosh ◽  
M.M. Parida ◽  
P.K. Dash ◽  
A. Pateriya ◽  
B. Pattnaik ◽  
...  
Keyword(s):  
Real Time ◽  
Chikungunya Virus ◽  
Sybr Green ◽  
Sybr Green I ◽  
Rt Pcr ◽  
Pcr Assay ◽  
One Step ◽  
Detection And Quantification

scholarly journals Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses

2021 ◽  
pp. 104063872199481
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Hangping Yao ◽  
Nanping Wu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Influenza A ◽  
Minor Groove Binder ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Pcr Assay ◽  
Infectious Dose ◽  
The Matrix ◽  
Taqman Minor Groove Binder

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

A one-step multiplex real-time RT-PCR assay for rapid and simultaneous detection of human norovirus genogroup I, II and IV

2013 ◽  
Vol 189 (2) ◽  
pp. 277-282 ◽  
Author(s):  
Yong Yan ◽  
Heng-hui Wang ◽  
Lei Gao ◽  
Ji-mei Ji ◽  
Zhi-jie Ge ◽  
...  
Keyword(s):  
Real Time ◽  
Simultaneous Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Human Norovirus ◽  
One Step
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