scholarly journals Subcutaneous Transplantation of Islets into Streptozocin-Induced Diabetic Rats

2005 ◽  
Vol 14 (8) ◽  
pp. 595-605 ◽  
Author(s):  
Craig R. Halberstadt ◽  
Deana Williams ◽  
Dwaine Emerich ◽  
Moses Goddard ◽  
Alfred V. Vasconcellos ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Cell Function ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Pancreatic Islet Transplantation ◽  
Minimally Invasive Surgical Procedure ◽  
Minimally Invasive Surgical ◽  
Ease Of Access ◽  
Syngeneic Islet Transplantation ◽  
Type 1 Diabetic Patients

Pancreatic islet transplantation into type 1 diabetic patients is currently being performed by intraportal infusion. This method, albeit reproducible, has some disadvantages including potential development of portal hypertension, hemorrhage, and an inability to retrieve or detect the transplanted tissue. Other transplant sites have been examined in animal models including the omentum, peritoneal cavity, and the spleen. A transplant site that has not been successful in supporting functional islet tissue transplantation in humans is the subcutaneous space due primarily to the lack of a well-defined vascular bed. This site has many favorable characteristics such as ease of access for transplantation and potential for removal of the transplanted tissue with a minimally invasive surgical procedure. This report addresses the evaluation of a subcutaneously placed device for the support of rat syngeneic islet transplantation in a streptozocin-induced diabetic model. The data generated support the use of this device for islet engraftment. In addition, beta cell function in this device compared favorably with the function of islets transplanted to the renal subcapsular space as well as islets within the native pancreas.

scholarly journals Pancreas Procurement and Preservation for Islet Transplantation: Personal Considerations

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Hirofumi Noguchi
Keyword(s):  
Islet Transplantation ◽  
Cooling System ◽  
Pancreatic Tissue ◽  
Diabetic Patients ◽  
Pancreatic Islet Transplantation ◽  
Ischemic Time ◽  
Trypsin Inhibition ◽  
Edmonton Protocol ◽  
Collagenase Inhibition ◽  
Type 1 Diabetic Patients

Pancreatic islet transplantation is a promising option for the treatment of type 1 diabetic patients. After the successful demonstration of the Edmonton protocol, islet transplantation has advanced significantly on several fronts, including improved pancreas procurement and preservation systems. Since we frequently use pancreata from donors after cardiac death in Japan,we have applied thein situregional organ cooling system for pancreas procurement to reduce the warm ischemic time. To reduce the apoptosis of pancreatic tissue during cold preservation, we have applied the ductal injection of preservation solution. For pancreas preservation, we use modified Kyoto solution, which is advantageous at trypsin inhibition and less collagenase inhibition. In this paper, we show pancreas procurement and preservation in our group for islet transplantation.

Insulin-mimetic effects of short-term Rapamycin in type 1 diabetic patients prior islet transplantation

2017 ◽  
Author(s):  
Federica Ermetici ◽  
Silvia Briganti ◽  
Stefano Benedini ◽  
Roberto Codella ◽  
Paola Maffi ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Diabetic Patients ◽  
Short Term ◽  
Insulin Mimetic ◽  
Type 1 Diabetic Patients

Pancreatic islet transplantation under the kidney capsule of mice: model of refinement for molecular and ex-vivo graft analysis

2021 ◽  
pp. 002367722110040
Author(s):  
Julien Thévenet ◽  
Valery Gmyr ◽  
Nathalie Delalleau ◽  
François Pattou ◽  
Julie Kerr-Conte
Keyword(s):  
Islet Transplantation ◽  
Pain Treatment ◽  
Ex Vivo ◽  
Well Being ◽  
Diabetic Patients ◽  
Mice Model ◽  
Pancreatic Islet Transplantation ◽  
Post Transplantation ◽  
Kidney Capsule

Diabetes cell therapy by human islet transplantation can restore an endogenous insulin secretion and normal glycaemic control in type 1 diabetic patients for as long as 10 years post transplantation. Before transplantation, each clinical islet preparation undergoes extensive in-vitro and in-vivo quality controls. The in-vivo quality control assay consists of transplanting human islets under the kidney capsule of immunocompromised mice. Currently, it is considered the best predictive factor to qualify clinical transplant efficiency. This chimeric model offers a wide area of study since it combines the possibility of producing not only quantitative but also a maximum of qualitative data. Today’s technological advances allow us to obtain more accurate and stronger data from the animals used in research while ensuring their comfort and well-being throughout the protocol, including cage enrichment and pain treatment during and after surgery. As demonstrated in this valuable model, we are able to generate more usable results (Refine), while reducing the number of animals used (Reduce), by focusing on the development of ex-vivo analysis techniques (Replace), which clearly highlights the Burch and Russell 3Rs concept.

scholarly journals Increased Plasma Amylin in Type 1 Diabetic Patients After Kidney and Pancreas Transplantation: A sign of impaired  -cell function?

Diabetes Care ◽  
2006 ◽  
Vol 29 (5) ◽  
pp. 1031-1038 ◽  
Author(s):  
M. Stadler ◽  
C. Anderwald ◽  
T. Karer ◽  
A. Tura ◽  
T. Kastenbauer ◽  
...  
Keyword(s):  
Pancreas Transplantation ◽  
Cell Function ◽  
Diabetic Patients ◽  
Type 1 Diabetic Patients

scholarly journals THERAPY OF ENDOCRINE DISEASE: Islet transplantation for type 1 diabetes: so close and yet so far away

2015 ◽  
Vol 173 (5) ◽  
pp. R165-R183 ◽  
Author(s):  
Mohsen Khosravi-Maharlooei ◽  
Ensiyeh Hajizadeh-Saffar ◽  
Yaser Tahamtani ◽  
Mohsen Basiri ◽  
Leila Montazeri ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Islet Transplantation ◽  
Diabetic Patients ◽  
Immune Rejection ◽  
Pancreatic Islet Transplantation ◽  
Insulin Independence ◽  
Endocrine Disease ◽  
The Past ◽  
Source Limitation

Over the past decades, tremendous efforts have been made to establish pancreatic islet transplantation as a standard therapy for type 1 diabetes. Recent advances in islet transplantation have resulted in steady improvements in the 5-year insulin independence rates for diabetic patients. Here we review the key challenges encountered in the islet transplantation field which include islet source limitation, sub-optimal engraftment of islets, lack of oxygen and blood supply for transplanted islets, and immune rejection of islets. Additionally, we discuss possible solutions for these challenges.

scholarly journals Laparoscopic approach for human islet transplantation into a defined liver segment in type-1 diabetic patients

2003 ◽  
Vol 16 (3) ◽  
pp. 186-190 ◽  
Author(s):  
Babak Movahedi ◽  
Bart Keymeulen ◽  
Mary-Helen Lauwers ◽  
Eva Goes ◽  
Nadine Cools ◽  
...  
Keyword(s):  
Islet Transplantation ◽  
Laparoscopic Approach ◽  
Human Islet ◽  
Diabetic Patients ◽  
Liver Segment ◽  
Human Islet Transplantation ◽  
Type 1 Diabetic Patients
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