Enhancement of Wound Healing by Human Multipotent Stromal Cell Conditioned Medium: The Paracrine Factors and p38 MAPK Activation

2011 ◽  
Vol 20 (5) ◽  
pp. 693-706 ◽  
Author(s):  
Tu-Lai Yew ◽  
Yeh-Ting Hung ◽  
Hsin-Yang Li ◽  
Hsin-Wei Chen ◽  
Ling-Lan Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Conditioned Medium ◽  
P38 Mapk ◽  
Stromal Cell ◽  
Paracrine Factors ◽  
Mapk Activation ◽  
Multipotent Stromal Cell ◽  
Cell Conditioned Medium

scholarly journals The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells

2019 ◽  
Vol 10 ◽  
pp. 204173141983339 ◽  
Author(s):  
Anny Waloski Robert ◽  
Felipe Azevedo Gomes ◽  
Michele Patricia Rode ◽  
Maiara Marques da Silva ◽  
Maria Beatriz da Rocha Veleirinho ◽  
...  
Keyword(s):  
Wound Healing ◽  
Conditioned Medium ◽  
Human Skin ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Multipotent Stromal Cells ◽  
Full Thickness ◽  
Multipotent Stromal Cell ◽  
Cell Conditioned Medium

Multipotent stromal cells stimulate skin regeneration after acute or chronic injuries. However, many stem cell therapy protocols are limited by the elevated number of cells required and poor cell survival after transplantation. Considering that the beneficial effects of multipotent stromal cells on wound healing are typically mediated by paracrine mechanisms, we examined whether the conditioned medium from skin-derived multipotent stromal cells would be beneficial for restoring the skin structure of mice after wounding. A proteomic characterization of skin-derived multipotent stromal cell-conditioned medium was performed, and the angiogenic function of this secretome was investigated in vitro using an endothelial cell tube formation assay. We then applied the skin-derived multipotent stromal cell-conditioned medium directly to full-thickness excisional wounds or embedded it into carrageenan or poly(vinyl alcohol) hydrogels to monitor tissue regeneration in mice. Biological processes related to wound healing and angiogenesis were highlighted by the analysis of the skin-derived multipotent stromal cell secretome, and a pro-angiogenic capacity for promoting tubule-like structures was first confirmed in vitro. Skin wounds treated with skin-derived multipotent stromal cell-conditioned medium also displayed increased angiogenesis, independently of the association of the conditioned medium with hydrogels. However, improvements in wound closure and epidermis or decreased inflammatory cell presence were not observed. Hence, the use of the secretome obtained from human skin-derived multipotent stromal cells may be a potential strategy to aid the natural skin repair of full-thickness lesions mainly based on its pro-angiogenic properties.

scholarly journals The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

2020 ◽  
Vol 10 (8) ◽  
pp. 2646
Author(s):  
Keng-Liang Ou ◽  
Yun-Wen Kuo ◽  
Chia-Yu Wu ◽  
Bai-Hung Huang ◽  
Fang-Tzu Pai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Conditioned Medium ◽  
Hair Growth ◽  
Hair Regeneration ◽  
Hacat Keratinocyte ◽  
Cell Conditioned Medium

The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.

Rat Bone Marrow Stromal Cell–Conditioned Medium Promotes Early Osseointegration of Titanium Implants

2013 ◽  
Vol 28 (5) ◽  
pp. 1360-1369 ◽  
Author(s):  
Shuhei Tsuchiya ◽  
Kenji Hara ◽  
Masayuki Ikeno ◽  
Yasuhiro Okamoto ◽  
Hideharu Hibi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Conditioned Medium ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Titanium Implants ◽  
Marrow Stromal Cell ◽  
Cell Conditioned Medium ◽  
Rat Bone

scholarly journals Mesenchymal Stem Cell/Multipotent Stromal Cell Augmentation of Wound Healing

2020 ◽  
Vol 190 (7) ◽  
pp. 1370-1381 ◽  
Author(s):  
Kyle Sylakowski ◽  
Andrew Bradshaw ◽  
Alan Wells
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Stromal Cell ◽  
Multipotent Stromal Cell

Human Bone Marrow Mesenchymal Stem Cell Conditioned Medium Promotes Wound Healing in Deep Second-Degree Burns in Male Rats

2018 ◽  
Vol 206 (6) ◽  
pp. 317-329 ◽  
Author(s):  
Arefeh Aryan ◽  
Mohammad Bayat ◽  
Shahin Bonakdar ◽  
Soudabeh Taheri ◽  
Newsha Haghparast ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Conditioned Medium ◽  
Wound Closure ◽  
Burn Injury ◽  
Human Bone ◽  
Male Rats ◽  
Cell Conditioned Medium

Burn wound treatment is difficult and one of the most challenging problems in the clinic. Researchers have examined the applications of mesenchymal stem cells as a cell-based therapy for skin regeneration. But the role of human bone marrow mesenchymal stem cell conditioned medium (hBM-MSC-CM) in the treatment of burn injury remains unclear. This research aims at detecting whether hBM-MSC-CM can increase the wound healing of deep second-degree burns in male rats. In this study, 32 adult male rats per each time point were randomly divided into four groups: (1) control group, (2) sham group (DMEM), (3) common treatment group (CT), and (4) conditioned media group (CM). A 3 × 3 cm circular burn was created on the back of the rats. On postsurgical days 7, 15, and 28, the wound closure area of each wound was measured and then the skin samples were removed and analyzed using stereological methods. Wound closure area was significantly increased in the CM and CT groups on the 15th and the 28th day after burn injury compared to the control and DMEM groups. The stereological parameters and immunohistochemistry analysis of the wounds revealed significantly improved healing in the CM group compared to the control and other groups. It is concluded that these findings indicate that hBM-MSC-CM promotes skin wound healing by increasing cell proliferation, regulating collagen synthesis and collagen composition, and inducing angiogenesis at the injury site.

scholarly journals Mesenchymal stem cell-conditioned medium accelerates wound healing with fewer scars

2015 ◽  
Vol 14 (1) ◽  
pp. 64-73 ◽  
Author(s):  
Meirong Li ◽  
Fuxin Luan ◽  
Yali Zhao ◽  
Haojie Hao ◽  
Jiejie Liu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Conditioned Medium ◽  
Cell Conditioned Medium
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