scholarly journals The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells

2019 ◽  
Vol 10 ◽  
pp. 204173141983339 ◽  
Author(s):  
Anny Waloski Robert ◽  
Felipe Azevedo Gomes ◽  
Michele Patricia Rode ◽  
Maiara Marques da Silva ◽  
Maria Beatriz da Rocha Veleirinho ◽  
...  
Keyword(s):  
Wound Healing ◽  
Conditioned Medium ◽  
Human Skin ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Multipotent Stromal Cells ◽  
Full Thickness ◽  
Multipotent Stromal Cell ◽  
Cell Conditioned Medium

Multipotent stromal cells stimulate skin regeneration after acute or chronic injuries. However, many stem cell therapy protocols are limited by the elevated number of cells required and poor cell survival after transplantation. Considering that the beneficial effects of multipotent stromal cells on wound healing are typically mediated by paracrine mechanisms, we examined whether the conditioned medium from skin-derived multipotent stromal cells would be beneficial for restoring the skin structure of mice after wounding. A proteomic characterization of skin-derived multipotent stromal cell-conditioned medium was performed, and the angiogenic function of this secretome was investigated in vitro using an endothelial cell tube formation assay. We then applied the skin-derived multipotent stromal cell-conditioned medium directly to full-thickness excisional wounds or embedded it into carrageenan or poly(vinyl alcohol) hydrogels to monitor tissue regeneration in mice. Biological processes related to wound healing and angiogenesis were highlighted by the analysis of the skin-derived multipotent stromal cell secretome, and a pro-angiogenic capacity for promoting tubule-like structures was first confirmed in vitro. Skin wounds treated with skin-derived multipotent stromal cell-conditioned medium also displayed increased angiogenesis, independently of the association of the conditioned medium with hydrogels. However, improvements in wound closure and epidermis or decreased inflammatory cell presence were not observed. Hence, the use of the secretome obtained from human skin-derived multipotent stromal cells may be a potential strategy to aid the natural skin repair of full-thickness lesions mainly based on its pro-angiogenic properties.

Enhancement of Wound Healing by Human Multipotent Stromal Cell Conditioned Medium: The Paracrine Factors and p38 MAPK Activation

2011 ◽  
Vol 20 (5) ◽  
pp. 693-706 ◽  
Author(s):  
Tu-Lai Yew ◽  
Yeh-Ting Hung ◽  
Hsin-Yang Li ◽  
Hsin-Wei Chen ◽  
Ling-Lan Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Conditioned Medium ◽  
P38 Mapk ◽  
Stromal Cell ◽  
Paracrine Factors ◽  
Mapk Activation ◽  
Multipotent Stromal Cell ◽  
Cell Conditioned Medium

scholarly journals The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

2020 ◽  
Vol 10 (8) ◽  
pp. 2646
Author(s):  
Keng-Liang Ou ◽  
Yun-Wen Kuo ◽  
Chia-Yu Wu ◽  
Bai-Hung Huang ◽  
Fang-Tzu Pai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Conditioned Medium ◽  
Hair Growth ◽  
Hair Regeneration ◽  
Hacat Keratinocyte ◽  
Cell Conditioned Medium

The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.

scholarly journals In vitro differentiation of human skin-derived multipotent stromal cells into putative endothelial-like cells

2012 ◽  
Vol 12 (1) ◽  
pp. 7 ◽  
Author(s):  
Radhakrishnan Vishnubalaji ◽  
Muthurangan Manikandan ◽  
May Al-Nbaheen ◽  
Balamuthu Kadalmani ◽  
Abdullah Aldahmash ◽  
...  
Keyword(s):  
Human Skin ◽  
Stromal Cells ◽  
Multipotent Stromal Cells ◽  
In Vitro Differentiation

Carrageenan hydrogel as a scaffold for skin-derived multipotent stromal cells delivery

2018 ◽  
Vol 33 (3) ◽  
pp. 422-434 ◽  
Author(s):  
Michele Patricia Rode ◽  
Addeli Bez Batti Angulski ◽  
Felipe Azevedo Gomes ◽  
Maiara Marques da Silva ◽  
Talita da Silva Jeremias ◽  
...  
Keyword(s):  
Human Skin ◽  
Delivery System ◽  
Stromal Cells ◽  
Kappaphycus Alvarezii ◽  
Skin Wound ◽  
Multipotent Stromal Cells ◽  
Skin Wound Healing ◽  
Matrix Deposition ◽  
Initial Recovery

Carrageenan is a thermoreversible polymer of natural origin widely used in food and pharmaceutical industry that presents a glycosaminoglycan-like structure. Herein, we show that kappa-type carrageenan extracted by a semi-refined process from the red seaweed Kappaphycus alvarezii displayed both chemical and structural properties similar to a commercial carrageenan. Moreover, both extracted carrageenan hydrogel and commercial carrageenan hydrogel can serve as a scaffold for in vitro culture of human skin-derived multipotent stromal cells, demonstrating considerable potential as cell-carrier materials for cell delivery in tissue engineering. Skin-derived multipotent stromal cells cultured inside the carrageenan hydrogels showed a round shape morphology and maintained their growth and viability for at least one week in culture. Next, the effect of the extracted carrageenan hydrogel loaded with human skin-derived multipotent stromal cells was evaluated in a mouse model of full-thickness skin wound. Macroscopic and histological analyses revealed some pointed ameliorated features, such as reduced inflammatory process, faster initial recovery of wounded area, and improved extracellular matrix deposition. These results indicate that extracted carrageenan hydrogel can serve as a scaffold for in vitro growth and maintenance of human SD-MSCs, being also able to act as a delivery system of cells to wounded skin. Thus, evaluation of the properties discussed in this study contribute to a further understanding and specificities of the potential use of carrageenan hydrogel as a delivery system for several applications, further to skin wound healing.

Cutaneous wound healing in the EpiDerm-FT full thickness in vitro human skin model

2010 ◽  
Vol 196 ◽  
pp. S152
Author(s):  
S. Letasiova ◽  
P. Hayden ◽  
G. Stolper ◽  
A. Armento ◽  
C. Cooney ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Cutaneous Wound Healing ◽  
Full Thickness ◽  
Skin Model ◽  
Cutaneous Wound

scholarly journals Amniotic fluid-derived multipotent stromal cells drive diabetic wound healing through modulation of macrophages

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Bibi S. Subhan ◽  
Jennifer Kwong ◽  
Joseph F. Kuhn ◽  
Arie Monas ◽  
Sonali Sharma ◽  
...  
Keyword(s):  
Wound Healing ◽  
Amniotic Fluid ◽  
Stromal Cells ◽  
Tissue Repair ◽  
Mouse Skin ◽  
Limiting Factors ◽  
Multipotent Stromal Cells ◽  
Diabetic Wound ◽  
Diabetic Wounds

Abstract Background Cutaneous wounds in patients with diabetes exhibit impaired healing due to physiological impediments and conventional care options are severely limited. Multipotent stromal cells (MSCs) have been touted as a powerful new therapy for diabetic tissue repair owing to their trophic activity and low immunogenicity. However, variations in sources and access are limiting factors for broader adaptation and study of MSC-based therapies. Amniotic fluid presents a relatively unexplored source of MSCs and one with wide availability. Here, we investigate the potential of amniotic fluid-derived multipotent stromal cells (AFMSCs) to restore molecular integrity to diabetic wounds, amend pathology and promote wound healing. Method We obtained third trimester amniotic fluid from term cesarean delivery and isolated and expanded MSCs in vitro. We then generated 10 mm wounds in Leprdb/db diabetic mouse skin, and splinted them open to allow for humanized wound modeling. Immediately after wounding, we applied AFMSCs topically to the sites of injuries on diabetic mice, while media application only, defined as vehicle, served as controls. Post-treatment, we compared healing time and molecular and cellular events of AFMSC-treated, vehicle-treated, untreated diabetic, and non-diabetic wounds. A priori statistical analyses measures determined significance of the data. Result Average time to wound closure was approximately 19 days in AFMSC-treated diabetic wounds. This was significantly lower than the vehicle-treated diabetic wounds, which required on average 27.5 days to heal (p < 0.01), and most similar to time of closure in wild type untreated wounds (an average of around 18 days). In addition, AFMSC treatment induced changes in the profiles of macrophage polarizing cytokines, resulting in a change in macrophage composition in the diabetic wound bed. We found no evidence of AFMSC engraftment or biotherapy induced immune response. Conclusion Treatment of diabetic wounds using amniotic fluid-derived MSCs encourages cutaneous tissue repair through affecting inflammatory cell behavior in the wound site. Since vehicle-treated diabetic wounds did not demonstrate accelerated healing, we determined that AFMSCs were therapeutic through their paracrine activities. Future studies should be aimed towards validating our observations through further examination of the paracrine potential of AFMSCs. In addition, investigations concerning safety and efficacy of this therapy in clinical trials should be pursued.

scholarly journals 721 Evaluation of wound healing in a full-thickness in vitro human skin model

2016 ◽  
Vol 136 (5) ◽  
pp. S127
Author(s):  
M.A. Bachelor ◽  
J. Oldach ◽  
G. Stolper ◽  
A. Armento ◽  
P. Hayden
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Full Thickness ◽  
Skin Model

scholarly journals Antler stem cell-conditioned medium stimulates regenerative wound healing in rats

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Xiaoli Rong ◽  
Wenhui Chu ◽  
Haiying Zhang ◽  
Yusu Wang ◽  
Xiaoyan Qi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Conditioned Medium ◽  
Dermal Fibroblasts ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Nih 3T3 ◽  
Cell Conditioned Medium

Abstract Background When the deer antler is cast, it leaves a cutaneous wound that can achieve scarless healing due to the presence of antler stem cells (ASCs). This provides an opportunity to study regenerative wound healing. Methods In this study, we investigated the therapeutic effects and mechanism of antler stem cell-conditioned medium (ASC-CM) on cutaneous wound healing in rats. In vitro, we investigated the effects of the ASC-CM on proliferation of HUVEC and NIH-3T3 cell lines. In vivo, we evaluated the effects of ASC-CM on cutaneous wound healing using full-thickness skin punch-cut wounds in rats. Results The results showed that ASC-CM significantly stimulated proliferation of the HUVEC and NIH-3T3 cells in vitro. In vivo, completion of healing of the rat wounds treated with ASC-CM was on day 16 (± 3 days), 9 days (± 2 days) earlier than the control group (DMEM); the area of the wounds treated with ASC-CM was significantly smaller (p < 0.05) than the two control groups. Further molecular characterization showed that the ratios of Col3A1/Col1A2, TGF-β3/TGF-β1, MMP1/TIMP1, and MMP3/TIMP1 significantly increased (p < 0.01) in the healed tissue in the ASC-CM group. Conclusions In conclusion, ASC-CM effectively accelerated the wound closure rate and enhanced the quality of healing, which might be through transforming wound dermal fibroblasts into the fetal counterparts. Therefore, the ASC-CM may have potential to be developed as a novel cell-free therapeutic for scarless wound healing.

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