scholarly journals COMPLEX TREATMENT OF NON-OBSTRUCTIVE FORMS OF MALE INFERTILITY WITH PLATELET-RICH PLASMA, LOWINTENSITY PULSED ULTRASOUND AND HUMAN PLACENTA HYDROLYSATE

Author(s):  
Ramzi Parviz ◽  
Ruslan Solomianyi ◽  
Yurii Zasieda

Relevance. Male infertility is basic problem for several medical specialties from genetics and endocrinology to urology and andrology. It’s impact on personal quality of life, social functioning and existential aspects of well being and in larger scale on social health is dramatic.Aim – to develop and test complex treatment model of non-obstructive forms of male infertility with combination of platelet-rich plasma, low-intensity pulsed ultrasound and human placenta hydrolysate.Methods. The study was based on prospective parallel group design. The study contingent consisted of 46 patients of the “Men’s Health Clinic” Kiev, Ukraine, undergoing outpatient treatment for non-obstructive fertility disorders.Following methods were used: clinical (a standard set of clinical examinations to establish a preliminary diagnosis), laboratory (bacterial seeding of ejaculate for the presence of pathogenic microflora, extended spermogram); serological (evaluations of serum testosterone and luteinizing hormone levels instrumental (sonographic examination of the prostate gland in order exclude prostatic inflammation); statistical.Results. After the initial complex of examinations study contingent underwent developed treatment protocol: 6 sessions (1 session per week) of local injections of 1ml HPH «Laennec»; 6 sessions of local injections of 1ml PRP (1 session per week); 6 sessions of LIPUS (1 session per week, following HPH and PRP injections); metabolic therapy: «SaluFertil Forte» and «SALUTRIB» 6 weeks daily.Conclusion. Complex treatment model of non-obstructive forms of male infertility with combination of platelet-rich plasma, low-intensity pulsed ultrasound and human placenta hydrolysate and metabolic therapy with «SaluFertil Forte» and «SALUTRIB», showed significant efficacy in 6-week therapeutic period. Therapeutic effect was found in sperm count in 1 ml (<0,01), sperm aggregation (<0,01) and sperm mobility (group A + B) (<0,01).

2021 ◽  
Vol 6 (11) ◽  
pp. 4073-4082
Author(s):  
Kunzhan Cai ◽  
Yilai Jiao ◽  
Quan Quan ◽  
Yulin Hao ◽  
Jie Liu ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tatsuya Shimizu ◽  
Naomasa Fujita ◽  
Kiyomi Tsuji-Tamura ◽  
Yoshimasa Kitagawa ◽  
Toshiaki Fujisawa ◽  
...  

AbstractUltrasound stimulation is a type of mechanical stress, and low-intensity pulsed ultrasound (LIPUS) devices have been used clinically to promote fracture healing. However, it remains unclear which skeletal cells, in particular osteocytes or osteoblasts, primarily respond to LIPUS stimulation and how they contribute to fracture healing. To examine this, we utilized medaka, whose bone lacks osteocytes, and zebrafish, whose bone has osteocytes, as in vivo models. Fracture healing was accelerated by ultrasound stimulation in zebrafish, but not in medaka. To examine the molecular events induced by LIPUS stimulation in osteocytes, we performed RNA sequencing of a murine osteocytic cell line exposed to LIPUS. 179 genes reacted to LIPUS stimulation, and functional cluster analysis identified among them several molecular signatures related to immunity, secretion, and transcription. Notably, most of the isolated transcription-related genes were also modulated by LIPUS in vivo in zebrafish. However, expression levels of early growth response protein 1 and 2 (Egr1, 2), JunB, forkhead box Q1 (FoxQ1), and nuclear factor of activated T cells c1 (NFATc1) were not altered by LIPUS in medaka, suggesting that these genes are key transcriptional regulators of LIPUS-dependent fracture healing via osteocytes. We therefore show that bone-embedded osteocytes are necessary for LIPUS-induced promotion of fracture healing via transcriptional control of target genes, which presumably activates neighboring cells involved in fracture healing processes.


Bone ◽  
2009 ◽  
Vol 45 (5) ◽  
pp. 862-869 ◽  
Author(s):  
Sjoerd Rutten ◽  
Peter A. Nolte ◽  
Clara M. Korstjens ◽  
Jenneke Klein-Nulend

Bone Reports ◽  
2021 ◽  
pp. 101122
Author(s):  
Yasamin Hadaegh ◽  
Hasan Uludag ◽  
Douglas Dederich ◽  
Tarek H. El-Bialy

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