Tissue Engineering - Collagen Matrix Based Flaps - a Simple Construct. Experimental and Theoretical Aspects

In our study we demonstrate the possibility to create a free flap using only a collagen matrix and a vascular pedicle. The processes of cell migration and differentiation, endogenous collagen synthesis, vascular network development, all rely on the arteriovenous loop introduced in the scaffold with no participation of surrounding vascularity. The flap has the capability to revascularize a full thickness skin graft, and also to establish vascular connections with adjacent vascular territories. Also, a fractal mathematical model was built based on spontaneous breaking symmetry to explain the behaviors of biostructures mentioned above.

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Tissue Engineering - Collagen Matrix Based Flaps - a Simple Construct. Experimental and Theoretical Aspects

Revista de Chimie ◽

10.37358/rc.19.10.3694 ◽

2019 ◽

Vol 70 (10) ◽

pp. 3694-3699

Keyword(s):

Tissue Engineering ◽

Free Flap ◽

Collagen Matrix ◽

Spontaneous Breaking ◽

Network Development ◽

Thickness Skin ◽

Vascular Connections ◽

Keywords Tissue Engineering ◽

Arteriovenous Loop ◽

Breaking Symmetry

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Effects of Caffeine Consumption on Autologous Full-Thickness Skin Graft Healing in an Animal Model

Indian Journal of Plastic Surgery ◽

10.1055/s-0041-1734573 ◽

2021 ◽

Author(s):

Tommy Supit ◽

Neni Susilaningsih ◽

Awal Prasetyo ◽

Keyword(s):

Wound Healing ◽

Skin Graft ◽

Collagen Synthesis ◽

High Dose ◽

Full Thickness ◽

Caffeine Consumption ◽

Full Thickness Skin Graft ◽

Full Thickness Skin ◽

Thickness Skin ◽

Medium Dose

Abstract Background There exists contradictory evidence that states both the beneficial and deleterious effects of caffeine on wound healing. The general population might unknowingly consume caffeine that negatively affects wound healing. The main objective of this study is to investigate the effect of daily caffeine consumption on wound healing, specifically full-thickness skin graft (FTSG). Methods Forty Sprague-Dawley rats were randomized into four groups of equal size: control-dose (CD), low-dose (LD), medium-dose (MD), and high-dose (HD) caffeine groups. After autologous FTSG, all subjects in the intervention group were given daily pure caffeine gavage. The FTSG was explanted 7 days posttransplant. The graft viability, secondary contraction, and adherence were evaluated macroscopically, while fibroblast and collagen deposition was analyzed microscopically with hematoxylin eosin stain. Results The least graft viability (72.8 ± 20.7%, clinical wound assessment scale [CWAS] 2.4), highest secondary contraction (11.4 ± 10.5%), and fibroblast count (331.8 ± 88.6 cells/5 high power fields) were observed in the MD group. More collagen synthesis was observed in subjects who consumed caffeine. The level of secondary contraction, fibroblast count as well as graft viability and collagen synthesis were positively correlated. Conclusions Daily consumption of caffeine impairs graft viability when given in medium dose and increases collagen synthesis, irrespective of dosage. This study was in experimental rats; the results are not directly translatable to humans.

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First experiences with a new surgical approach in adult full-thickness burns: single step reconstruction of epidermal, dermal and subcutaneous defects by use of split-thickness skin grafting, a dermal collagen matrix and autologous fat-transfer

European Surgery ◽

10.1007/s10353-013-0211-2 ◽

2013 ◽

Vol 45 (5) ◽

pp. 263-269 ◽

Cited By ~ 5

Author(s):

M. Keck ◽

H. F . Selig ◽

J. Kober ◽

D. B . Lumenta ◽

H. Schachner ◽

...

Keyword(s):

Skin Grafting ◽

Collagen Matrix ◽

Single Step ◽

Full Thickness ◽

Fat Transfer ◽

Autologous Fat ◽

Autologous Fat Transfer ◽

Thickness Skin ◽

Split Thickness Skin Grafting ◽

Dermal Collagen

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Early wound repair versus later scar repair in children with treadmill hand friction burns

Journal of Burn Care & Research ◽

10.1093/jbcr/irab083 ◽

2021 ◽

Author(s):

Rong Zhou ◽

Lin Qiu ◽

Jun Xiao ◽

Xiaobo Mao ◽

Xingang Yuan

Keyword(s):

Skin Graft ◽

Wound Repair ◽

Full Thickness ◽

Full Thickness Skin Graft ◽

Vacuum Sealing ◽

Full Thickness Skin ◽

Thickness Skin ◽

Vacuum Sealing Drainage ◽

Repair Group ◽

Hand Friction

Abstract The incidence of pediatric treadmill hand friction burns has been increasing every year. The injuries are deeper than thermal hand burns, the optimal treatment remains unclear. This was a retrospective study of children who received surgery for treadmill hand friction burns from January 1, 2015, to December 31, 2019, in a single burn center. A total of 22 children were surveyed. The patients were naturally divided into two groups: the wound repair group (13 patients), which was admitted early to the hospital after injury and received debridement and vacuum sealing drainage initially, and a full-thickness skin graft later; and the scar repair group (9 patients), in which a scar contracture developed as a result of wound healing and received scar release and skin grafting later. The Modified Michigan Hand Questionnaire score in the wound repair group was 116.31 ± 10.55, and the corresponding score in the scar repair group was 117.56 ± 8.85 (P>0.05), no statistically significant difference. The Vancouver Scar Scale score in the wound repair group was 4.15 ± 1.21, and the corresponding score in the scar repair group was 7.22 ± 1.09 (P<0.05). Parents were satisfied with the postoperative appearance and function of the hand. None in the two groups required secondary surgery. If the burns are deep second degree, third degree, or infected, early debridement, vacuum sealing drainage initially, and a full-thickness skin graft can obviously relieve pediatric pain, shorten the course of the disease, and restore the function of the hand as soon as possible.

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