Effects of Erxian Decoction and its separate prescriptions on the levels of luteinizing hormone and follicle-stimulating hormone in anterior pituitary cells from female rats

2007 ◽  
Vol 5 (6) ◽  
pp. 665-669 ◽  
Author(s):  
Binfeng Dong
Keyword(s):  
Luteinizing Hormone ◽  
Anterior Pituitary ◽  
Follicle Stimulating Hormone ◽  
Female Rats ◽  
Pituitary Cells ◽  
Erxian Decoction ◽  
Anterior Pituitary Cells ◽  
Stimulating Hormone

Inhibition of release of a novel pituitary polypeptide, 7B2, follicle-stimulating hormone, and luteinizing hormone from rat anterior pituitary cells in vitro by human β-inhibin

1986 ◽  
Vol 64 (9) ◽  
pp. 1259-1262 ◽  
Author(s):  
John S. D. Chan ◽  
Jie-Ying Deng ◽  
Anoop K. Brar ◽  
Nabil G. Seidah ◽  
Michel Chrétien
Keyword(s):  
Luteinizing Hormone ◽  
Anterior Pituitary ◽  
Polyclonal Antibodies ◽  
Follicle Stimulating Hormone ◽  
Feedback Mechanism ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells ◽  
Specific Radioimmunoassay ◽  
Stimulating Hormone

We have recently purified a novel pituitary polypeptide designated 7B2. By raising polyclonal antibodies to a synthetic 7B2 fragment in rabbits, we have developed a sensitive and specific radioimmunoassay for this novel polypeptide, and it has been used for the study of the release of immunoreactive 7B2 from rat anterior pituitary cells in vitro. In addition, immunocytochemical study shows that 7B2 is present in the gonadotropin cells of rat anterior pituitary. The aim of the present studies is to investigate the effect of human β-inhibin, testosterone, and combined testosterone plus human β-inhibin on the induced release of immunoreactive 7B2, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) in rat anterior pituitary cell culture in vitro. Our results show that both human β-inhibin and testosterone effectively suppress the stimulatory effect of luteinizing hormone-releasing hormone (LHRH) on immunoreactive 7B2, FSH, and LH release. The present data indicate that the regulation of secretion of 7B2 and pituitary gonadotropins may be under a similar type of feedback mechanism.

scholarly journals Differential Expression of c-fos In Vitro by All Anterior Pituitary Cell Types During the Estrous Cycle: Enhanced Expression by Luteinizing Hormone but Not by Follicle-stimulating Hormone Cells

1997 ◽  
Vol 45 (6) ◽  
pp. 785-794 ◽  
Author(s):  
Jennifer Armstrong ◽  
Gwen V. Childs
Keyword(s):  
Luteinizing Hormone ◽  
Differential Expression ◽  
Estrous Cycle ◽  
Anterior Pituitary ◽  
Follicle Stimulating Hormone ◽  
Cell Types ◽  
Pituitary Cells ◽  
Fos Expression ◽  
Lh Cells ◽  
Stimulating Hormone

C-fos expression appears in some activated cell types. Because of dynamic changes in gonadotropes during the estrous cycle, this study was initiated to determine if fos might be expressed in gonadotropes before any period of activation. We detected c-fos and pituitary antigens in dissociated anterior pituitary cells by dual-labeling immunocy-tochemistry. The highest percentages of cells with fos protein were found in proestrous rat populations. In diestrous and proestrous populations, dual labeling showed that 6–9% of pituitary cells contained fos with adrenocorticotropin, thyroid-stimulating hormone, prolactin, or growth hormone antigens. In contrast, only 0.8–3% contained fos with luteinizing hormone (LH) or follicle-stimulating hormone (FSH) antigens. We then tested the hypothesis that gonadotropes might increase fos expression earlier in the cycle. In populations from metestrous rats, c-fos labeling was found in 45% of LH cells compared to only 23% of LH cells in the proestrous group. This suggests that proportionately more LH cells are being activated to produce fos early in the cycle. Perhaps fos is used in translation of LHβ antigens or gonadotropin-releasing hormone (GnRH) receptor mRNAs. In contrast, less than 1% of all pituitary cells expressed fos with FSH at all stages of the cycle (only 6–12% of FSH cells). This differential expression suggests one mechanism behind the regulation of non-parallel storage and release of gonadotropin antigens.

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