scholarly journals Artificial feeding of Ornithodoros fonsecai (Acari: Argasidae) with the anticoagulant Alsever

2021 ◽  
Vol 3 ◽  
pp. ec03047
Author(s):  
Ana C. Castro-Santiago ◽  
Simone M. Simons ◽  
Leidiane Lima-Duarte ◽  
Jaqueline V. Camargo ◽  
Rosangela Z. Machado ◽  
...  

Ornithodoros fonsecai is a species of argasid tick endemic to Brazil, described in the “São Miguel” cave located in the municipality of Bonito, state of Mato Grosso do Sul, central-western region of Brazil. The artificial feeding technique makes it possible to study the biology of hematophagous arthropods using artificial or natural membranes, as well as different types of blood and anticoagulants. Thus, the aim of the present study was to feed artificially O. fonsecai second instar (N2) nymphs with rabbit blood using parafilm membrane and the anticoagulant Alsever. Ninety percent of the total N2 nymphs engorged and molted to N3 nymphs between 27 and 30 days after feeding, indicating that the use of this anticoagulant is efficient for artificially feeding O. fonsecai N2 nymphs under laboratory conditions.

Hoehnea ◽  
2017 ◽  
Vol 44 (3) ◽  
pp. 361-377 ◽  
Author(s):  
Vera Lucia Ramos Bononi ◽  
Ademir Kleber Morbeck de Oliveira ◽  
Adriana de Melo Gugliotta ◽  
Josiane Ratier de Quevedo

ABSTRACT The fungi diversity in Brazil is not fully known yet, mainly in Serra de Maracaju, which is located in the central portion of the State of Mato Grosso do Sul, in the center-western region of Brazil. Samples were taken from different phytophysiognomies of the Cerrado, the dominating biome of that region, in areas where Cerrado and pasture alternate, in the municipality of Corguinho. Of the species identified, 18 are new citations for Brazil, as they are not included in the List of Brazilian Flora (fungi), and 36 are recorded for the first time for [the State of] Mato Grosso do Sul. As a total, 62 species were collected in nine excursions during 2014 and 2015. Out of this total, 15 species are deemed edible, four are toxic, ten are medicinal, two are used in bioremediation processes, and one is bioluminescent, according to the literature.


2016 ◽  
Vol 64 (2) ◽  
pp. 547 ◽  
Author(s):  
Sergio Roberto Rodrigues ◽  
Kleyton Rezende Ferreira ◽  
Elias Soares Gomes

Members of the genus <em>Leucothyreus</em> Macleay, are little investigated, therefore, studies were conducted in the municipality of Aquidauana and Cassilândia, Mato Grosso do Sul State, Brazil, to analyze the biological aspects and mating behavior in <em>Leucothyreus albopilosus</em>. Adults were captured in a light trap. Afterwards, they were sexed and couples were isolated in the laboratory for reproduction and mating behavior. The biological studies started from eggs under laboratory conditions. In the field, we observed that adults were collected mostly in warmer and wetter periods, from August to March. The embryonic period of <em>L</em>. <em>albopilosus</em> lasted 20.5 days, the 1<sup>st</sup>, 2<sup>nd</sup> and 3<sup>rd</sup> instars lasted 31.0, 33.1 and 85.6 days, respectively. The pupal stage lasted 20 days and the egg to adult period was completed in 185.5 days. These results suggest that <em>L.</em> <em>albopilosus</em> can be characterized as univoltine specie. In laboratory, the adults performed the flying action after the sunset and normally the male seeks the female to start mating. The copulations occurred from 19:00 to 23:00 hours and lasted 19,45 minutes in average. The female is able to refuse the male in order to not start the copulation process. Adults were observed feeding on ripe bananas (<em>Musa</em> sp., Musaceae) and on mangaba flowers (<em>Hancornia speciosa</em>,<em> </em>Apocynaceae).


2009 ◽  
Vol 54 (4) ◽  
Author(s):  
Marcelo Labruna ◽  
Jose Venzal

AbstractMales, females, and larvae of Carios fonsecai sp. nov. are described from free-living ticks collected in a cave at Bonito, state of Mato Grosso do Sul, Brazil. The presence of cheeks and legs with micromammillate cuticle makes adults of C. fonsecai morphologically related to a group of argasid species (mostly bat-associated) formerly classified into the subgenus Alectorobius, genus Ornithodoros. Examination of larvae indicates that C. fonsecai is clearly distinct from most of the previously described Carios species formerly classified into the subgenus Alectorobius, based primarily on its larger body size, dorsal setae number, dorsal plate shape, and hypostomal morphology. On the other hand, the larva of C. fonsecai is most similar to Carios peropteryx, and Carios peruvianus, from which differences in dorsal plate length and width, tarsal setae, and hypostome characteristics are useful for morphological differentiation. The mitochondrial 16S rDNA sequence of C. fonsecai showed to be closest (85–88% identity) to several corresponding sequences of different Carios species available in GenBank. Bats identified as Peropteryx macrotis and Desmodus rotundus were found infested by C. fonsecai larvae in the same cave where the type series was collected. C. fonsecai showed to be aggressive to humans in the laboratory.


1998 ◽  
Vol 40 (3) ◽  
pp. 151-154 ◽  
Author(s):  
Iray Maria ROCCO ◽  
Maria Luisa BARBOSA ◽  
Emilia Hiromi Nakaya KANOMATA

Dengue outbreaks have occurred in several Brazilian States since 1986 involving serotypes 1 (DEN-1) and 2 (DEN-2). In view of the few cases of double infection documented in the literature, we report here a case of simultaneous infection with DEN-1 and DEN-2 in a patient residing in the municipality of Miranda, State of Mato Grosso do Sul, Western region of Brazil. DEN-1 was introduced in this State in 1989 and DEN-2 in 1996, both of them circulating in some municipalities. This double infection was identified by virus isolation and by indirect immunofluorescence using monoclonal antibodies and confirmed by the polymerase chain reaction (PCR). This is the first documented case of simultaneous infection with serotypes DEN-1 and DEN-2 in Brazil.


2020 ◽  
Vol 45 (2) ◽  
pp. 340-348
Author(s):  
James Lucas da Costa-Lima ◽  
Earl Celestino de Oliveira Chagas

Abstract—A synopsis of Dicliptera (Acanthaceae) for Brazil is presented. Six species are recognized: Dicliptera ciliaris, D. sexangularis, and D. squarrosa, widely distributed in South America; D. purpurascens, which ranges from the North Region of Brazil (in the state of Acre) to eastern Bolivia; D. gracilirama, a new species from the Atlantic Forest of northeastern Brazil; and D. granchaquenha, a new species recorded in dry and semideciduous forests in Bolivia and western Brazil, in the state of Mato Grosso do Sul. Furthermore, we propose new synonyms and designate lectotypes for eleven names. An identification key to the six accepted Dicliptera species in Brazil is provided.


Author(s):  
F. PERBONI ◽  
Carla Regina de Souza FIGUEIREDO ◽  
A. MARQUES ◽  
A. N. MILITÃO ◽  
C. N. JESUS ◽  
...  

2012 ◽  
Vol 11 (1) ◽  
Author(s):  
Paulo Roberto Cimo Queiroz

O espaço correspondente ao atual Estado brasileiro de Mato Grosso do Sul foi incorporado, no século XVI, aos circuitos do Paraguai colonial, mas, já no século XVII, no contexto que S. B. de Holanda denomina refluxo assuncenho, a presença espanhola foi sendo substituída pela luso-brasileira, passando, portanto, essa região a vincular-se, ainda que de m o do inicialmente tênue, ao sudeste da América portuguesa. Em meados d o século XIX, c om a liberação da navegação brasileira pelo rio Paraguai, essa região voltou, de certo modo, a fazer parte do espaço platino. O presente trabalho busca evidenciar que, a despeito das notáveis mudanças induzidas pela livre navegação, esse último período de vinculação ao espaço platino constituiu, na verdade, uma espécie de "hiato", no interior do processo mais longo, representado pela vinculação com o mercado nacional brasileiro.


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