scholarly journals SIMULTANEOUS INFECTION WITH DENGUE 1 AND 2 IN A BRAZILIAN PATIENT

1998 ◽  
Vol 40 (3) ◽  
pp. 151-154 ◽  
Author(s):  
Iray Maria ROCCO ◽  
Maria Luisa BARBOSA ◽  
Emilia Hiromi Nakaya KANOMATA
Keyword(s):  
Virus Isolation ◽  
Western Region ◽  
Double Infection ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Brazilian Patient ◽  
Dengue Outbreaks ◽  
Simultaneous Infection ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

Dengue outbreaks have occurred in several Brazilian States since 1986 involving serotypes 1 (DEN-1) and 2 (DEN-2). In view of the few cases of double infection documented in the literature, we report here a case of simultaneous infection with DEN-1 and DEN-2 in a patient residing in the municipality of Miranda, State of Mato Grosso do Sul, Western region of Brazil. DEN-1 was introduced in this State in 1989 and DEN-2 in 1996, both of them circulating in some municipalities. This double infection was identified by virus isolation and by indirect immunofluorescence using monoclonal antibodies and confirmed by the polymerase chain reaction (PCR). This is the first documented case of simultaneous infection with serotypes DEN-1 and DEN-2 in Brazil.

scholarly journals Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

2019 ◽  
Vol 28 (1) ◽  
pp. 80-90 ◽  
Author(s):  
Jamille Batista Faria Prado ◽  
Carlos Alberto do Nascimento Ramos ◽  
Vagner Ricardo da Silva Fiuza ◽  
Veronica Jorge Babo Terra
Keyword(s):  
Enterocytozoon Bieneusi ◽  
Domestic Cat ◽  
Domestic Cats ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Source Of Infection ◽  
Two Samples ◽  
Intestinal Pathogen ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

Abstract Enterocytozoon bieneusi is an opportunistic intestinal pathogen that infects humans and a wide variety of animals worldwide. Our aim in this study was to investigate the occurrence of E. bieneusi in a domestic cat population in Campo Grande, Mato Grosso do Sul, Brazil. Sixty fecal samples from diarrheic cats were subjected to polymerase chain reaction (PCR) and the amplicons were sequenced for identification. E. bieneusi was detected in two samples (3.3%), both identified as genotype D. This genotype has already been reported in animals and humans and is considered a zoonotic genotype. Our findings represent the first report of E. bieneusi in domestic cats in Brazil, reinforcing the importance of identifying this agent as a source of infection in animals and humans.

scholarly journals Identification of class 1 and 2 integrons from clinical and environmental Salmonella isolates

2014 ◽  
Vol 8 (12) ◽  
pp. 1518-1524 ◽  
Author(s):  
Fábio Ederson Lopes Corrêa ◽  
Fabiana Gomes da Silva Dantas ◽  
Alexeia Barufatti Grisolia ◽  
Bruno do Amaral Crispim ◽  
Kelly Mari Pires Oliveira
Keyword(s):  
Chain Reaction ◽  
Mato Grosso ◽  
Future Studies ◽  
Minimum Inhibitory Concentrations ◽  
Class 1 ◽  
Resistant Strains ◽  
Polymerase Chain ◽  
Different Sources ◽  
Mato Grosso Do Sul

Introduction: The indiscriminate use of antimicrobials has selected for the emergence of resistant strains. Many mechanisms contribute to the spread of antimicrobial-resistant genes, and integrons play a key role in this process. The aim of this study was to describe the serotypes and resistance profiles, and to characterize the presence of integrons in Salmonella strains isolated from Dourados, Mato Grosso do Sul, Brazil. Methodology: Thirty-six isolates from different sources were used. To evaluate the resistance profiles, the determination of minimum inhibitory concentrations together with polymerase chain reaction were used to screen for the presence of class 1 and class 2 integrons. Results: The Infantis serotype of Salmonella was the most frequently isolated serotype. Minimum inhibitory concentrations showed that out of the 36 isolates, 11 (30.5%) were resistant to all the antimicrobials tested. These resistant isolates were separated into three groups: 4 clinical isolates (36.4%), 3 food isolates (36.4%), and 4 water isolates (27.2%). Class 1 integrons occurred in 31 (86.1%) isolates and were found in all 11 resistant isolates (35.5 %) and in 20 (64.5%) of the non-resistant isolates. Class 2 integrons were found in 3 (8.3%) isolates, which were all non-resistant. Conclusion: The presence of an integron did not necessarily confer resistance. Future studies will seek to identify the mechanism behind integron-mediated antimicrobial resistance.

scholarly journals Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction

2011 ◽  
Vol 106 (6) ◽  
pp. 763-768 ◽  
Author(s):  
Anahí Souto Vieira ◽  
Grácia Maria Soares Rosinha ◽  
Carina Elisei de Oliveira ◽  
Silvio Arruda Vasconcellos ◽  
Paulo Andre Lima-Borges ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
The State ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Ozotoceros Bezoarticus ◽  
Leptospira Spp ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

scholarly journals Occurrence of Mycoplasma haemocanis in dogs infested by ticks in Campo Grande, Mato Grosso do Sul, Brazil

2016 ◽  
Vol 25 (3) ◽  
pp. 360-363 ◽  
Author(s):  
Rodrigo Leite Soares ◽  
Jessica Teles Echeverria ◽  
Giovana Pazzuti ◽  
Herbert Patric Kellerman Cleveland ◽  
Verônica Jorge Babo-Terra ◽  
...  
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Epidemiological Factors ◽  
Epidemiological Features ◽  
Blood Sucking ◽  
Rhipicephalus Sanguineus Sensu Lato ◽  
Dog Blood ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

Abstract Hemotropic mycoplasmas in dogs, such as Mycoplasma haemocanis, have been described worldwide. Recently, these pathogens have been reported to be causative agent of zoonosis. It is known that its transmission may occur through the action of blood-sucking arthropods (e.g. ticks or fleas), through blood transfusion, contaminated fomites and/or transplacentally. In Brazil, M. haemocanis is present in practically all regions and the tick Rhipicephalus sanguineus sensu lato is suspected the main vector. In the municipality of Campo Grande, state of Mato Grosso do Sul, there is little information about infection of dogs by M. haemocanis, or on the main epidemiological features associated with it. Thus, the aim of the present study was to determine the occurrence of M. haemocanis among dogs infested by ticks and to assess possible associations with some epidemiological factors. The polymerase chain reaction (PCR) and DNA sequencing were used to analyze dog blood samples (n = 94). DNA from M. haemocanis was detected in four samples. No significant associations were observed with any epidemiological parameter analyzed here. However, the results from this study confirm that this pathogen is circulating in this region and should be considered in the differential diagnosis of diseases among anemic dogs.

Detection of Zika Virus Replication in Human Semen by Reverse-Transcription Polymerase Chain Reaction Targeting of Antisense Ribonucleic Acid

2020 ◽  
Vol 222 (2) ◽  
pp. 319-323 ◽  
Author(s):  
Ralph Huits ◽  
Birgit De Smet ◽  
Gilda Grard ◽  
Kaat Eggermont ◽  
Catherine Minto-Bain ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Zika Virus ◽  
Ribonucleic Acid ◽  
Virus Isolation ◽  
Threshold Values ◽  
Chain Reaction ◽  
Zikv Infection ◽  
Isolation Methods ◽  
Polymerase Chain

Abstract Background Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection. Methods We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples. Results We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV loads (cycle threshold values <26) and were obtained within 21 days of symptom onset. Conclusions The sensitivity of the asRNA assay for detection of ZIKV replication was higher than that of conventional virus isolation methods (47.4% vs 21.1%, P = .032).

scholarly journals Evaluation of a 384–Well Format for High-Throughput Real-Time Reverse Transcription Polymerase Chain Reaction for Avian Influenza Testing

2009 ◽  
Vol 21 (5) ◽  
pp. 679-683 ◽  
Author(s):  
Pamela J. Ferro ◽  
Jason Osterstock ◽  
Bo Norby ◽  
Geoffrey T. Fosgate ◽  
Blanca Lupiani
Keyword(s):  
Polymerase Chain Reaction ◽  
Avian Influenza ◽  
Real Time ◽  
High Throughput ◽  
Reverse Transcription ◽  
Virus Isolation ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Response Planning ◽  
Polymerase Chain

As concerns over the global spread of highly pathogenic avian influenza H5N1 have heightened, more countries are faced with increased surveillance efforts and incident response planning for handling a potential outbreak. The incorporation of molecular techniques in most diagnostic laboratories has enabled fast and efficient testing of many agents of concern, including avian influenza. However, the need for high-throughput testing remains. In this study, the use of a 384–well format for high-throughput real-time reverse transcription polymerase chain reaction (real-time RT-PCR) testing for avian influenza is described. The analytical sensitivity of a real-time RT-PCR assay for avian influenza virus matrix gene with the use of both 96– and 384–well assay formats and serial dilutions of transcribed control RNA were comparable, resulting in similar limits of detection. Of 28 hunter-collected cloacal swabs that were positive by virus isolation, 26 (92.9%) and 27 (96.4%) were positive in the 96– and 384–well assays, respectively; of the 340 hunter-collected swabs that were negative by virus isolation, 45 (13.2%) and 23 (6.8%) were positive in the 96– and 384–well assays, respectively. The data presented herein supports the utility of the 384–well format in the event of an avian influenza outbreak for high-throughput real-time RT-PCR testing.

scholarly journals HCV Seroreactivity and Detection of HCV RNA in Hepatitis Cases

2018 ◽  
Vol 54 (02) ◽  
pp. 074-077
Author(s):  
Mahantesh B Nagamoti ◽  
Chidanand S Patil ◽  
Jyoti M Nagamoti
Keyword(s):  
Polymerase Chain Reaction ◽  
Liver Biopsy ◽  
Hcv Rna ◽  
Western Region ◽  
Liver Pathology ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Cellular Carcinoma ◽  
Routine Investigations

ABSTRACT Background: Hepatitis C virus (HCV) known to be associated with wide variety of liver pathology. It is less studied in India as compared to western region. Methods: Suspected patients sera screened for HCV by ELISA and confirmed with reverse transcription polymerase chain reaction (RT-PCR) along with routine investigations and liver profile. All HCV positive patients were undergone liver biopsy. Results: All 24 HCV ELISA reactive and two ELISA indeterminate sera are confirmed by RT- PCR. The liver biopsy of these patients showed normal picture (19.2%), Acute hepatitis (11.5%), Chronic hepatitis (23.7%), Cirrhosis (34.72%), Hepato-cellular carcinoma (HCC) (15.38%). ALT levels were not significant. Conclusion: All the suspected HCV cases need to be confirmed for HCV by RT-PCR.

scholarly journals Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil

2013 ◽  
Vol 22 (1) ◽  
pp. 136-142 ◽  
Author(s):  
Antonio Thadeu Medeiros Barros ◽  
Teresinha Tizu Sato Schumaker ◽  
Wilson Werner Koller ◽  
Guilherme Marcondes Klafke ◽  
Thais Aguiar de Albuquerque ◽  
...  
Keyword(s):  
Pyrethroid Resistance ◽  
The State ◽  
Kdr Mutation ◽  
Low Frequencies ◽  
Mato Grosso ◽  
Pyrethroid Insecticides ◽  
Resistance Factors ◽  
Horn Fly ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

Horn fly resistance to pyrethroid insecticides occurs throughout Brazil, but knowledge about the involved mechanisms is still in an incipient stage. This survey was aimed to identify the mechanisms of horn fly resistance to cypermethrin in Mato Grosso do Sul state, Brazil. Impregnated filter paper bioassays using cypermethrin, synergized or not with piperonyl butoxide (PBO) and triphenyl phosphate (TPP), were conducted from March 2004 to June 2005 in horn fly populations (n = 33) from all over the state. All populations were highly resistant to cypermethrin, with resistance factors (RF) ranging from 89.4 to 1,020.6. Polymerase chain reaction (PCR) assays to detect the knockdown resistance (kdr) mutation also were performed in 16 samples. The kdr mutation was found in 75% of the tested populations, mostly with relatively low frequencies (<20%), and was absent in some highly resistant populations. Addition of TPP did not significantly reduce the LC50 in any population. However, PBO reduced LC50s above 40-fold in all tested populations, resulting in RFs ≤ 10 in most cases. Horn fly resistance to cypermethrin is widespread in the state, being primarily caused by an enhanced activity of P450 mono-oxygenases and secondarily by reduced target site sensitivity.

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