The preponderance of susceptible sour orange (Citrus aurantium L.) rootstock has facilitated the spread of Citrus tristeza virus (CTV) in Trinidad and Tobago. CTV is transmitted by the brown citrus aphid Toxoptera citricida (Kirkaldy), which establishes large colonies on new flushes of citrus plants. As the colonies become highly populated, winged (alate) aphids are produced which can migrate to uninfected neighbouring citrus trees and hence transmit CTV. In the present study different concentrations of the entomopathogenic fungus Verticillium lecanii (Zimm) Viegas in water-based formulations were applied to T. citricida and the pathogenic effects were analysed. Bioassays were also conducted on the major parasitoid Lysiphlebus testaceipes Cresson to test the pathogenic effects of V. lecanii on mortality and the percent emergence from mummies. The LC<sub>50</sub> values for T. citricida and L. testaceipes were 2.26 × 10<sup>10</sup> spores/ml and 1.09 × 10<sup>9</sup> spores/ml, respectively. Statistical analyses indicated that there was no significant difference between the two LC<sub>50</sub> values. At the highest concentration (1.49 × 10<sup>9</sup> spores/ml) percent mortality peaked at 78.9% after 12 days and 1.95 × 10<sup>9</sup> spores/ml, mortality of L. testaceipes reached 95.1% after 6 days. Emergence of L. testaceipes at the highest concentration of 1.95 × 10<sup>9</sup> spores/ml was 57.8%.
High efficiency genetic transformation of sour orange (Citrus aurantium) and production of transgenic trees containing the coat protein gene of citrus tristeza virus