scholarly journals Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

10.3791/3987 ◽  
2012 ◽  
Author(s):  
Xiaofeng Zheng ◽  
Guang Hu
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Reporter Assay ◽  
Self Renewal ◽  
Stem Cell Maintenance ◽  
Cell Maintenance

scholarly journals Methionine metabolism is essential for SIRT 1‐regulated mouse embryonic stem cell maintenance and embryonic development

2017 ◽  
Vol 36 (21) ◽  
pp. 3175-3193 ◽  
Author(s):  
Shuang Tang ◽  
Yi Fang ◽  
Gang Huang ◽  
Xiaojiang Xu ◽  
Elizabeth Padilla‐Banks ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Development ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Methionine Metabolism ◽  
Stem Cell Maintenance ◽  
Cell Maintenance

scholarly journals The Role of Ubiquitination in Regulating Embryonic Stem Cell Maintenance and Cancer Development

2019 ◽  
Vol 20 (11) ◽  
pp. 2667 ◽  
Author(s):  
Dian Wang ◽  
Fan Bu ◽  
Weiwei Zhang
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cancer Biology ◽  
Embryonic Stem ◽  
Developmental Defects ◽  
Stem Cell Maintenance ◽  
Substrate Protein ◽  
Cellular Events ◽  
Ubiquitin Conjugating Enzymes ◽  
Cell Maintenance

Ubiquitination regulates nearly every aspect of cellular events in eukaryotes. It modifies intracellular proteins with 76-amino acid polypeptide ubiquitin (Ub) and destines them for proteolysis or activity alteration. Ubiquitination is generally achieved by a tri-enzyme machinery involving ubiquitin activating enzymes (E1), ubiquitin conjugating enzymes (E2) and ubiquitin ligases (E3). E1 activates Ub and transfers it to the active cysteine site of E2 via a transesterification reaction. E3 coordinates with E2 to mediate isopeptide bond formation between Ub and substrate protein. The E1-E2-E3 cascade can create diverse types of Ub modifications, hence effecting distinct outcomes on the substrate proteins. Dysregulation of ubiquitination results in severe consequences and human diseases. There include cancers, developmental defects and immune disorders. In this review, we provide an overview of the ubiquitination machinery and discuss the recent progresses in the ubiquitination-mediated regulation of embryonic stem cell maintenance and cancer biology.

Combining competition assays with genetic complementation strategies to dissect mouse embryonic stem cell self-renewal and pluripotency

2012 ◽  
Vol 7 (4) ◽  
pp. 729-748 ◽  
Author(s):  
Dung-Fang Lee ◽  
Jie Su ◽  
Ana Sevilla ◽  
Julian Gingold ◽  
Christoph Schaniel ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Genetic Complementation ◽  
Self Renewal ◽  
Competition Assays

scholarly journals Activation of PDK-1 maintains mouse embryonic stem cell self-renewal in a PKB-dependent manner

Oncogene ◽  
2013 ◽  
Vol 32 (47) ◽  
pp. 5397-5408 ◽  
Author(s):  
L S Ling ◽  
D Voskas ◽  
J R Woodgett
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Dependent Manner ◽  
Self Renewal

scholarly journals Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal

2018 ◽  
Vol 69 (6) ◽  
pp. 1028-1038.e6 ◽  
Author(s):  
Jing Wen ◽  
Ruitu Lv ◽  
Honghui Ma ◽  
Hongjie Shen ◽  
Chenxi He ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Self Renewal ◽  
Nuclear Rna

scholarly journals Wnt/β-catenin and LIF–Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal

2015 ◽  
Vol 129 (2) ◽  
pp. 269-276 ◽  
Author(s):  
Shoudong Ye ◽  
Dongming Zhang ◽  
Fei Cheng ◽  
Daniel Wilson ◽  
Jeffrey Mackay ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Signaling Pathways ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Self Renewal ◽  
Stat3 Signaling

scholarly journals Effects of Oxidative Stress on Mouse Embryonic Stem Cell Proliferation, Apoptosis, Senescence, and Self-Renewal

2010 ◽  
Vol 19 (9) ◽  
pp. 1321-1331 ◽  
Author(s):  
Yan-Lin Guo ◽  
Samujjwal Chakraborty ◽  
Suja S. Rajan ◽  
Rouxing Wang ◽  
Faqing Huang
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Self Renewal ◽  
Stem Cell Proliferation

Screening for Genes Essential for Mouse Embryonic Stem Cell Self-Renewal Using a Subtractive RNA Interference Library

Stem Cells ◽  
2006 ◽  
Vol 24 (12) ◽  
pp. 2661-2668 ◽  
Author(s):  
Jun-Zheng Zhang ◽  
Wei Gao ◽  
Hong-Bo Yang ◽  
Bo Zhang ◽  
Zuo-Yan Zhu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Rna Interference ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Self Renewal

scholarly journals The effects of silver nanoparticles on mouse embryonic stem cell self-renewal and proliferation

2015 ◽  
Vol 2 ◽  
pp. 758-764 ◽  
Author(s):  
Pavan Rajanahalli ◽  
Christopher J. Stucke ◽  
Yiling Hong
Keyword(s):  
Silver Nanoparticles ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Self Renewal
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