Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients

10.3791/58507 ◽  
2019 ◽  
Author(s):  
Riccardo Pinciroli ◽  
Simone Checchi ◽  
Maurizio Bottiroli ◽  
Gianpaola Monti ◽  
Giampaolo Casella ◽  
...  
Keyword(s):  
Critically Ill ◽  
Critically Ill Patients ◽  
Whole Blood ◽  
Activity Assay ◽  
Endotoxin Activity Assay ◽  
Endotoxin Activity

scholarly journals Endotoxin activity assay and polymyxin B hemoperfusion use in a cohort of critically ill patients

Critical Care ◽  
2014 ◽  
Vol 18 (Suppl 1) ◽  
pp. P408
Author(s):  
SL Cutuli ◽  
G De Pascale ◽  
V Alicino ◽  
S Cicconi ◽  
V Di Gravio ◽  
...  
Keyword(s):  
Critically Ill ◽  
Critically Ill Patients ◽  
Polymyxin B ◽  
Activity Assay ◽  
Endotoxin Activity Assay ◽  
Endotoxin Activity ◽  
Polymyxin B Hemoperfusion

scholarly journals Usefulness of the endotoxin activity assay as a biomarker to assess the severity of endotoxemia in critically ill patients

2014 ◽  
Vol 20 (8) ◽  
pp. 881-887 ◽  
Author(s):  
Toshiaki Ikeda ◽  
Kazumi Ikeda ◽  
Shingo Suda ◽  
Takuya Ueno
Keyword(s):  
Critically Ill ◽  
Critically Ill Patients ◽  
Activity Assay ◽  
Endotoxin Activity Assay ◽  
Endotoxin Activity

CLINICAL USEFULNESS OF NEWLY DEVELOPED WHOLE BLOOD ENDOTOXIN ACTIVITY ASSAY (EAA).

2005 ◽  
Vol 33 ◽  
pp. A163
Author(s):  
Kazuaki Atagi ◽  
Natsuko Tokuhira ◽  
Atsushi Ujiro ◽  
Hideki Shimaoka
Keyword(s):  
Whole Blood ◽  
Clinical Usefulness ◽  
Activity Assay ◽  
Endotoxin Activity Assay ◽  
Endotoxin Activity

scholarly journals Endotoxin Activity in Patients With Extracorporeal Membrane Oxygenation Life Support: An Observational Pilot Study

2021 ◽  
Vol 8 ◽  
Author(s):  
Chen-Tse Lee ◽  
Chih-Hsien Wang ◽  
Wing-Sum Chan ◽  
Yun-Yi Tsai ◽  
Tzu-Jung Wei ◽  
...  
Keyword(s):  
Pilot Study ◽  
Extracorporeal Membrane Oxygenation ◽  
Clinical Outcomes ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Life Support ◽  
Median Difference ◽  
Endotoxin Activity ◽  
Va Ecmo ◽  
Vv Ecmo

Background: Extracorporeal membrane oxygenation (ECMO) life support has become an integral part of intensive care. The endotoxin activity assay (EAA) is a useful test to measure endotoxemia severity in whole blood. To date, no information is available regarding the EAA levels and their effect on clinical outcomes in critically ill patients with ECMO support.Methods: This prospective observational pilot study enrolled adult critically ill patients with ECMO support from August 2019 to December 2020. The EAA levels were measured within 24 h (T1), and at 25–48 (T2), 49–72 (T3), and 73–96 h (T4) after ECMO initiation. This study primarily aimed to investigate the incidence of high EAA levels (≥0.6) at each time point. Subsequent exploratory analyses were conducted to compare the EAA levels of venoarterial ECMO (VA-ECMO) patients between 30-day survivors and non-survivors. Post-hoc analysis was performed to compare the clinical outcomes of VA-ECMO patients with elevated EAA levels at T3 (vs. T1) and those without elevated EAA levels.Results: A total of 39 VA-ECMO patients and 15 venovenous ECMO (VV-ECMO) patients were enrolled. At T1, the incidence of high EAA level (≥0.6) was 42% in VV-ECMO patients and 9% in VA-ECMO patients (P = 0.02). At T2, the incidence of high EAA level was 40% in VV-ECMO patients and 5% in VA-ECMO patients (P = 0.005). In VA-ECMO patients, EAA levels at T3 were significantly higher in 30-day non-survivors than in survivors (median [interquartile range]: 0.49 [0.37–0.93] vs. 0.31 [0.19–0.51], median difference 0.16 [95% confidence interval [CI], 0.02–0.31]; P = 0.024). Moreover, VA-ECMO patients with elevated EAA levels at T3 (vs. T1) had lower 30-day survival than patients without elevated EAA levels (39 vs. 83%, P = 0.026) and fewer ECMO free days by day 30 (median: 3 vs. 23 days, median difference 12 days [95% CI, 0–22]; P = 0.028).Conclusions: A certain proportion of patients experienced high EAA levels (≥0.6) after VV-ECMO or VA-ECMO initiation. VA-ECMO patients with an elevated EAA level at 49–72 h were associated with poor clinical outcomes.

Severity and prognostic assessment of the endotoxin activity assay in biliary tract infection

2013 ◽  
Vol 21 (2) ◽  
pp. 120-127 ◽  
Author(s):  
Mari Sato ◽  
Ryusei Matsuyama ◽  
Toshiaki Kadokura ◽  
Ryutaro Mori ◽  
Takafumi Kumamoto ◽  
...  
Keyword(s):  
Tract Infection ◽  
Biliary Tract ◽  
Activity Assay ◽  
Biliary Tract Infection ◽  
Endotoxin Activity Assay ◽  
Prognostic Assessment ◽  
Endotoxin Activity

scholarly journals Inhibition of the Defense System Stimulating Interleukin-12 Interferon-γ Pathway During Critical Illness

Blood ◽  
1997 ◽  
Vol 89 (5) ◽  
pp. 1612-1620 ◽  
Author(s):  
Wolfgang Ertel ◽  
Marius Keel ◽  
Regula Neidhardt ◽  
Ursula Steckholzer ◽  
Jean-Pierre Kremer ◽  
...  
Keyword(s):  
Critical Illness ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Whole Blood ◽  
Interferon Γ ◽  
Interleukin 12 ◽  
High Susceptibility ◽  
Healthy Humans ◽  
Ifn Γ ◽  
Tgf Β1

Abstract Interleukin-12 (IL-12) and interferon-γ (IFN-γ) exert protective effects during experimental endotoxemia through upregulation of cellular immunity and phagocytic functions. They are part of a positive regulatory feedback loop that enhances the production of the other. Because critically ill patients show a marked suppression of T-cell and macrophage functions with a high susceptibility to infection, potential defects in the immunity/inflammation upregulating IL-12 IFN-γ pathway were studied. As an ex vivo model of endotoxemia, lipopolysaccharide (LPS) stimulated whole blood from 25 critically ill patients and 12 healthy individuals was incubated with either recombinant human (rh) IL-12 or rhIFN-γ, respectively. IFN-γ dose-dependently (P < .05) increased the release of IL-12 p40 and p70 into LPS-stimulated whole blood from healthy humans without effect in whole blood from critically ill patients. RhIL-12 p70 enhanced (P < .05) the secretion of IFN-γ in controls, while it was ineffective in LPS-stimulated whole blood from critically ill patients. The observed inhibition of the IL-12 IFN-γ pathway is not specific to LPS, since Staphylococcus aureus Cowan strain I (SAC)-stimulated whole blood from critically ill patients showed similar suppression. The secretion of IL-12 and IFN-γ was less reduced in critically ill patients when using isolated cultures of adherent cells or lymphocytes. Although preculture of whole blood from healthy humans with IL-10, but not with IL-4, mimicked suppression of the IL-12 IFN-γ pathway similar to that observed during critical illness, the release of antiinflammatory reacting cytokines (IL-4, IL-10, transforming growth factor [TGF]-β1 ) was decreased into LPS-stimulated whole blood from critically ill patients. These results indicate at least two mechanisms responsible for dramatic disturbances of the IL-12 IFN-γ pathway during critical illness: (1) deactivation of IL-12 and IFN-γ producing leukocytes in vivo early after the primary insult, and (2) presence of serum suppressive factors different from IL-4, IL-10, or TGF-β1 . Because IL-12 and IFN-γ upregulate essential immune functions, the marked inhibition of IL-12 and IFN-γ release may be pivotal for high susceptibility of critically ill patients to infection.

scholarly journals Endotoxin Activity Assay: An Experience of a Southern-Italy Hospital

2014 ◽  
Vol 10 (4) ◽  
pp. 164-166
Author(s):  
Linda Degl’Innocenti ◽  
Flaviano Di Paola ◽  
Rizzo Marianna ◽  
Gerardino Amato
Keyword(s):  
Southern Italy ◽  
Activity Assay ◽  
Endotoxin Activity Assay ◽  
Endotoxin Activity

scholarly journals Newly developed endotoxin measurement method (endotoxin activity assay) may reflect the severity of sepsis

Critical Care ◽  
2009 ◽  
Vol 13 (Suppl 1) ◽  
pp. P381 ◽  
Author(s):  
H Murayama ◽  
Y Kakihana ◽  
T Oryoji ◽  
N Kiyonaga ◽  
S Tashiro ◽  
...  
Keyword(s):  
Measurement Method ◽  
Activity Assay ◽  
Endotoxin Activity Assay ◽  
Endotoxin Activity

scholarly journals Investigation into problems associated with the endotoxin activity assay

Critical Care ◽  
2011 ◽  
Vol 15 (S3) ◽  
Author(s):  
N Matsumoto ◽  
G Takahashi ◽  
M Kojika ◽  
Y Ishibe ◽  
S Tatsuyori ◽  
...  
Keyword(s):  
Activity Assay ◽  
Endotoxin Activity Assay ◽  
Endotoxin Activity
Sign in / Sign up

Export Citation Format

Share Document