Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

10.3791/60498 ◽  
2020 ◽  
Author(s):  
Yong Wang ◽  
Ding Zhang ◽  
Fang Zhou ◽  
Meijun Zhou ◽  
Qiujie Li ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Vascular Development ◽  
Tube Formation ◽  
Zebrafish Embryos ◽  
Tube Formation Assay ◽  
Whole Mount

scholarly journals Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues

Biology Open ◽  
2012 ◽  
Vol 1 (6) ◽  
pp. 566-569 ◽  
Author(s):  
A. K. Lagendijk ◽  
J. D. Moulton ◽  
J. Bakkers
Keyword(s):  
In Situ Hybridization ◽  
Zebrafish Embryos ◽  
Adult Tissues ◽  
Whole Mount ◽  
Hybridization Protocol

Abstract 448: Etv2-Mir130a-Jarid2 Cascade Regulates Vascular Patterning During Embryogenesis

2017 ◽  
Vol 37 (suppl_1) ◽  
Author(s):  
Bhairab N Singh ◽  
Naoyuki Tahara ◽  
Yasuhiko Kawakami ◽  
Naoko Koyano-Nakagawa ◽  
Wuming Gong ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Critical Role ◽  
Yolk Sac ◽  
Zebrafish Embryos ◽  
Vascular Patterning ◽  
Downstream Target

Remodeling of the pre-existing primitive vasculature is necessary for the formation of a complex branched vascular architecture. However, the factors that modulate these processes are incompletely defined. Previously, we defined the role of microRNAs (miRNAs) in endothelial specification. In the present study, we further examined the Etv2-Cre mediated ablation of Dicer L/L and characterized the perturbed vascular patterning in the embryo proper and yolk-sac. We mechanistically defined an important role for miR-130a , an Etv2 downstream target, in the mediation of vascular patterning and angiogenesis in vitro and in vivo . Inducible overexpression of miR-130a resulted in robust induction of vascular sprouts and angiogenesis with increased uptake of acetylated-LDL. Mechanistically, miR-130a directly regulates Jarid2 expression by binding to its 3’-UTR region. CRISPR/Cas9 mediated knockout of miR-130a showed increased levels of Jarid2 in the ES/EB system. Further, the levels of Jarid2 transcripts were increased in the Etv2-null embryos at E8.5. In the in vivo settings, injection of miR-130a specific morpholinos in zebrafish embryos resulted in perturbed vascular patterning with reduced levels of endothelial transcripts in the miR-130a morphants. qPCR and in situ hybridization techniques demonstrated increased expression of jarid2a in the miR-130a morphants in vivo . These findings demonstrate a critical role for Etv2-miR-130a-Jarid2 in vascular patterning both in vitro and in vivo .

Combined whole-mount fluorescence in situ hybridization and antibody staining in zebrafish embryos and larvae

2020 ◽  
Vol 15 (10) ◽  
pp. 3361-3379
Author(s):  
Jianbo He ◽  
Dashuang Mo ◽  
Jingying Chen ◽  
Lingfei Luo
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Zebrafish Embryos ◽  
Antibody Staining ◽  
Whole Mount

High-resolution in situ hybridization to whole-mount zebrafish embryos

2007 ◽  
Vol 3 (1) ◽  
pp. 59-69 ◽  
Author(s):  
Christine Thisse ◽  
Bernard Thisse
Keyword(s):  
In Situ Hybridization ◽  
High Resolution ◽  
Zebrafish Embryos ◽  
Whole Mount
Sign in / Sign up

Export Citation Format

Share Document