Measurement of Glutamate Uptake using Radiolabeled L-[3H]-Glutamate in Acute Transverse Slices Obtained from Rodent Resected Hippocampus

10.3791/62292 ◽  
2021 ◽  
Author(s):  
Débora G. Souza ◽  
Yasmine Nonose ◽  
Diogo O. Souza ◽  
Roberto F. Almeida
Keyword(s):  
Glutamate Uptake

Glutamate Uptake in Synaptic Plasticity: From Mollusc to Mammal

2002 ◽  
Vol 2 (7) ◽  
pp. 593-603 ◽  
Author(s):  
Jonathan Levenson ◽  
Edwin Weeber ◽  
J. Sweatt ◽  
Arnold Eskin
Keyword(s):  
Synaptic Plasticity ◽  
Glutamate Uptake

Inhibition of short-term memory formation in the chick by blockade of extracellular glutamate uptake

2004 ◽  
Vol 81 (2) ◽  
pp. 115-119 ◽  
Author(s):  
M.E Gibbs ◽  
L Hertz ◽  
K.T Ng
Keyword(s):  
Short Term Memory ◽  
Glutamate Uptake ◽  
Memory Formation ◽  
Short Term ◽  
Extracellular Glutamate ◽  
Term Memory

scholarly journals Glutamate uptake by brain synaptic vesicles. Energy dependence of transport and functional reconstitution in proteoliposomes.

1988 ◽  
Vol 263 (30) ◽  
pp. 15423-15428
Author(s):  
P R Maycox ◽  
T Deckwerth ◽  
J W Hell ◽  
R Jahn
Keyword(s):  
Energy Dependence ◽  
Synaptic Vesicles ◽  
Glutamate Uptake

scholarly journals Astrocyte Glutamate Uptake and Water Homeostasis Are Dysregulated in the Hippocampus of Multiple Sclerosis Patients With Seizures

ASN NEURO ◽  
2020 ◽  
Vol 12 ◽  
pp. 175909142097960
Author(s):  
Andrew S. Lapato ◽  
Sarah M. Thompson ◽  
Karen Parra ◽  
Seema K. Tiwari-Woodruff
Keyword(s):  
Multiple Sclerosis ◽  
Mouse Model ◽  
Regional Differences ◽  
Neuronal Excitability ◽  
Demyelinating Disease ◽  
Glutamate Uptake ◽  
Cx43 Expression ◽  
Water Homeostasis ◽  
Hippocampal Astrocytes ◽  
Multiple Sclerosis Patients

While seizure disorders are more prevalent among multiple sclerosis (MS) patients than the population overall and prognosticate earlier death & disability, their etiology remains unclear. Translational data indicate perturbed expression of astrocytic molecules contributing to homeostatic neuronal excitability, including water channels (AQP4) and synaptic glutamate transporters (EAAT2), in a mouse model of MS with seizures (MS+S). However, astrocytes in MS+S have not been examined. To assess the translational relevance of astrocyte dysfunction observed in a mouse model of MS+S, demyelinated lesion burden, astrogliosis, and astrocytic biomarkers (AQP4/EAAT2/ connexin-CX43) were evaluated by immunohistochemistry in postmortem hippocampi from MS & MS+S donors. Lesion burden was comparable in MS & MS+S cohorts, but astrogliosis was elevated in MS+S CA1 with a concomitant decrease in EAAT2 signal intensity. AQP4 signal declined in MS+S CA1 & CA3 with a loss of perivascular AQP4 in CA1. CX43 expression was increased in CA3. Together, these data suggest that hippocampal astrocytes from MS+S patients display regional differences in expression of molecules associated with glutamate buffering and water homeostasis that could exacerbate neuronal hyperexcitability. Importantly, mislocalization of CA1 perivascular AQP4 seen in MS+S is analogous to epileptic hippocampi without a history of MS, suggesting convergent pathophysiology. Furthermore, as neuropathology was concentrated in MS+S CA1, future study is warranted to determine the pathophysiology driving regional differences in glial function in the context of seizures during demyelinating disease.

Characterization of glutamate uptake systems in astrocyte primary cultures from rat brain

Glia ◽  
1991 ◽  
Vol 4 (3) ◽  
pp. 293-304 ◽  
Author(s):  
Berenike Flott ◽  
Wilfried Seifert
Keyword(s):  
Rat Brain ◽  
Glutamate Uptake ◽  
Primary Cultures

Glutamate Uptake by the Isolated Toad Brain

Science ◽  
1973 ◽  
Vol 181 (4102) ◽  
pp. 860-862 ◽  
Author(s):  
R. P. Shank ◽  
J. T. Whiten ◽  
C. F. Baxter
Keyword(s):  
Glutamate Uptake

Riluzole increases high-affinity glutamate uptake in rat spinal cord synaptosomes

2000 ◽  
Vol 871 (2) ◽  
pp. 175-180 ◽  
Author(s):  
R.D Azbill ◽  
X Mu ◽  
J.E Springer
Keyword(s):  
Spinal Cord ◽  
Glutamate Uptake ◽  
Rat Spinal Cord ◽  
High Affinity
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