scholarly journals Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

10.3791/62901 ◽  
2021 ◽  
Author(s):  
Janna Leiz ◽  
Christian Hinze ◽  
Anastasiya Boltengagen ◽  
Caroline Braeuning ◽  
Christine Kocks ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Adult Mouse ◽  
Mouse Kidney ◽  
Nuclei Isolation ◽  
Single Nucleus

scholarly journals Single-Cell RNA Sequencing of the Adult Mouse Kidney: From Molecular Cataloging of Cell Types to Disease-Associated Predictions

2019 ◽  
Vol 73 (1) ◽  
pp. 140-142 ◽  
Author(s):  
Nils O. Lindström ◽  
Guilherme De Sena Brandine ◽  
Andrew Ransick ◽  
Andrew P. McMahon
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Adult Mouse ◽  
Cell Types ◽  
Mouse Kidney ◽  
Single Cell Rna Sequencing

scholarly journals The molecular taxonomy of primate amygdala via single-nucleus RNA-sequencing analysis

2021 ◽  
Author(s):  
Lei Zhang ◽  
Yanyong Cheng ◽  
Shihao Wu ◽  
Yufeng Lu ◽  
Zhenyu Xue ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Molecular Taxonomy ◽  
Sequencing Analysis ◽  
Single Nucleus

scholarly journals A developmentally plastic adult mouse kidney cell line spontaneously generates multiple adult kidney structures

2015 ◽  
Vol 463 (4) ◽  
pp. 1334-1340 ◽  
Author(s):  
Carol F. Webb ◽  
Michelle L. Ratliff ◽  
Rebecca Powell ◽  
Celeste R. Wirsig-Wiechmann ◽  
Olga Lakiza ◽  
...  
Keyword(s):  
Cell Line ◽  
Kidney Cell ◽  
Adult Mouse ◽  
Mouse Kidney ◽  
Kidney Cell Line ◽  
Adult Kidney

Single-nucleus RNA-sequencing of human choriodecidua before and after the onset of labor

Placenta ◽  
2021 ◽  
Vol 112 ◽  
pp. e67
Author(s):  
Léa Chicoisne ◽  
Muriel Andrieu ◽  
Céline Bertholle ◽  
Vaarany Karunanithy ◽  
Brigitte Izac ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Before And After ◽  
Single Nucleus

scholarly journals Single nucleus and in situ RNA sequencing reveals cell topographies in the human pancreas

2020 ◽  
Author(s):  
Luca Tosti ◽  
Yan Hang ◽  
Olivia Debnath ◽  
Sebastian Tiesmeyer ◽  
Timo Trefzer ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Human Pancreas ◽  
Single Nucleus

scholarly journals Nuclei Isolation for Single-Nuclei RNA sequencing v1 (protocols.io.bkacksaw)

protocols.io ◽  
2020 ◽  
Author(s):  
Danh Truong ◽  
Salah Eddine ◽  
Joseph A
Keyword(s):  
Rna Sequencing ◽  
Nuclei Isolation

scholarly journals Single Cell, Single Nucleus and Spatial RNA Sequencing of the Human Liver Identifies Hepatic Stellate Cell and Cholangiocyte Heterogeneity

2021 ◽  
Author(s):  
Tallulah S Andrews ◽  
Jawairia Atif ◽  
Jeff C Liu ◽  
Catia T Perciani ◽  
Xue-Zhong Ma ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Human Liver ◽  
Stellate Cell ◽  
Parenchymal Cell ◽  
Cell Types ◽  
Cell Populations ◽  
Healthy Human ◽  
Single Nucleus

The critical functions of the human liver are coordinated through the interactions of hepatic parenchymal and non-parenchymal cells. Recent advances in single cell transcriptional approaches have enabled an examination of the human liver with unprecedented resolution. However, dissociation related cell perturbation can limit the ability to fully capture the human liver's parenchymal cell fraction, which limits the ability to comprehensively profile this organ. Here, we report the transcriptional landscape of 73,295 cells from the human liver using matched single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA sequencing (snRNA-seq). The addition of snRNA-seq enabled the characterization of interzonal hepatocytes at single-cell resolution, revealed the presence of rare subtypes of hepatic stellate cells previously only seen in disease, and detection of cholangiocyte progenitors that had only been observed during in vitro differentiation experiments. However, T and B lymphocytes and NK cells were only distinguishable using scRNA-seq, highlighting the importance of applying both technologies to obtain a complete map of tissue-resident cell-types. We validated the distinct spatial distribution of the hepatocyte, cholangiocyte and stellate cell populations by an independent spatial transcriptomics dataset and immunohistochemistry. Our study provides a systematic comparison of the transcriptomes captured by scRNA-seq and snRNA-seq and delivers a high-resolution map of the parenchymal cell populations in the healthy human liver.

scholarly journals Adult mouse kidney stem cell‐derived exosomes attenuate blood pressure and improve kidney functions in aged mice

2021 ◽  
Vol 35 (S1) ◽  
Author(s):  
Xiaobin Han ◽  
Leah Akinseye ◽  
Zhongjie Sun
Keyword(s):  
Blood Pressure ◽  
Stem Cell ◽  
Adult Mouse ◽  
Mouse Kidney ◽  
Aged Mice ◽  
Kidney Functions

EPCO-07. HYBRID NEURO-GLIAL CELLULAR ARCHITECTURE IN HIGH-GRADE GLIOMA DRIVEN BY H3-G34R MUTATION

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi2-vi2
Author(s):  
Julie Laffy ◽  
Masashi Nomura ◽  
Chen He ◽  
Lillian Bussema ◽  
Michal Slyper ◽  
...  
Keyword(s):  
Young Adults ◽  
Rna Sequencing ◽  
Developmental Trajectories ◽  
High Grade Glioma ◽  
Cerebral Hemispheres ◽  
High Grade ◽  
Malignant Cells ◽  
Aberrant Expression ◽  
Cellular Architecture ◽  
Single Nucleus

Abstract High-grade gliomas (HGG) with histone H3.3 G34R mutation are rare intractable tumours in the cerebral hemispheres that preferentially affect adolescents and young adults, but have unknown mechanisms of neuroanatomical specificity and tumourigenesis. Here, we performed single-nucleus RNA-sequencing of twenty patient samples, encompassing twelve tumours with G34R mutation and eight H3.3 wildtype HGGs, age- and location-matched. Both classes of HGG were heterogeneous, with malignant cells in multiple states, recapitulating neural and glial developmental trajectories. G34R HGG is distinguished by lack of malignant cells in the oligodendroglial lineage, and aberrant expression of neuronal programs superimposed over cellular states, resulting in hybrid glio-neuronal malignant programs. Singe-cell barcoding supports plasticity between cellular states in HGG with multiple possible transitions. CRISPR-correction of G34R in HGG models followed by scRNA-seq supports that the G34R mutation directly drives these aberrant programs. Our study provides a framework for studying the origin and tumourigenesis of paediatric gliomas.

Sign in / Sign up

Export Citation Format

Share Document