scholarly journals Design of Experiment (DOE) Utilization to Develop a Simple and Robust Reversed-Phase HPLC Technique for Related Substances’ Estimation of Omeprazole Formulations

2013 ◽  
Vol 81 (4) ◽  
pp. 1043-1056 ◽  
Author(s):  
Vayeda Chintan Manranjan
Keyword(s):  
Design Of Experiment ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Related Substances ◽  
Hplc Technique

scholarly journals Single-run reversed-phase HPLC method for determining sertraline content, enantiomeric purity, and related substances in drug substance and finished product

2020 ◽  
Vol 10 (6) ◽  
pp. 610-616
Author(s):  
Alessia Rosetti ◽  
Rosella Ferretti ◽  
Leo Zanitti ◽  
Adriano Casulli ◽  
Claudio Villani ◽  
...  
Keyword(s):  
Reversed Phase ◽  
Hplc Method ◽  
Enantiomeric Purity ◽  
Drug Substance ◽  
Reversed Phase Hplc ◽  
Related Substances

scholarly journals A Validated Reversed-Phase HPLC Analytical Method for the Analysis of Fenofibrate in Bulk Drug and Tablet Dosage Formulation

2021 ◽  
pp. 306-312
Author(s):  
Awdhut Pimpale ◽  
Rajendra Kakde ◽  
Ishwar Kakde
Keyword(s):  
Solvent System ◽  
Reversed Phase ◽  
Pharmaceutical Sciences ◽  
Reversed Phase Hplc ◽  
System Suitability ◽  
Water Ph ◽  
Hplc Technique ◽  
Bulk Drug ◽  
Tablet Dosage ◽  
Dosage Formulation

Aims: A accurate, precise, and stability-indicating Reversed-Phase HPLC technique has been established for the estimation of fenofibrate in tablet formulation. Study Design:  Experimental study. Place and Duration of Study: Department of Pharmaceutical Sciences, RTM Nagpur University, Nagpur-440033, Maharashtra, India between June 2019 and March 2020. Methodology: The chromatographic separation was attained on RP Princeton column (C18) (250 mm x 4.6 mm, 5 µ) with mobile solvent system as a mixture of water (pH 3.0 along o-phosphoric acid) and acetonitrile in the proportion (40:60) v/v, flow rate 1.0 ml per minute, at 240 nm. The retention time of fenofibrate was 3.905 minutes. Results: The method demonstrated linearity in the concentration range of 87-232 µg/ml with a coefficient of correlation (r2) of 0.9994. The % RSD was ˂2% and percentage recovery was found to be 99.13-100.74%. The assay of marketed tablet formulations was found to be 99.98%. Conclusion: The developed and validated technique as per ICH rules for specificity, accuracy, precision, linearity, and system suitability. Reverse Phase-HPLC technique was utilized to the market formulation.

scholarly journals Rapid quantification of Psilocybin with reversed-phase HPLC and single-wavelength detection

2021 ◽  
Author(s):  
Alexzander Samuelsson ◽  
Eric Janusson ◽  
Sajni Shah ◽  
Markus Roggen
Keyword(s):  
High Throughput ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
New Methods ◽  
Purity Analysis ◽  
Hplc Technique ◽  
Wavelength Detection ◽  
Analytical Requirements ◽  
Rapid Quantification

The alkaloid psilocybin (4-phosphoryloxy-N,N-dimethyltryptamine) and the neurologically active psilocin (4-hydroxy-N,N-dimethyltryptamine) are the foremost compounds of pharmaceutical interest in Psilocybe mushrooms. As these compounds are infrequently analyzed in analytical labs, validated methods for rapid purity analysis are lacking. Newfound therapeutic use has invigorated academic and commercial interests in the molecules and new methods of production and available products are expanding. As a result, high-throughput methods of analysis for psilocybin must be improved to promptly determine chemical differences between mushroom genera or other sources of psilocybin and psilocin, as well as refined product purity. To address this, we developed an inexpensive HPLC technique for the efficient quantification of psilocybin and psilocin by using readily available equipment and dilute reagents. Aqueous ammonium formate (0.143 mM) was found to be preferable over techniques with much higher buffer concentrations or stronger acids for controlling psilocybin Zwitterion resolution. The chromatographic run time satisfied high-throughput analytical requirements with an efficient total runtime under 2 minutes. A standard octadecyl silica (C18) column provided excellent resolution between psilocybin and psilocin signals. The quality of the method was validated using certified analytical reference standards and was found to be accurate (3.5% bias, Psilocybin), reliable (0.32% RSD), and efficient (Psilocybin k’ = 1.78).

scholarly journals A Reversed-phase HPLC Study of the Complexation of Benzene Derivatives Guest Molecules with 5,17-bis(N-Tolyliminomethyl)-25,27-dipropoxycalix[4]arene in Acetonitrile–Water Solution

1999 ◽  
Vol 23 (1) ◽  
pp. 60-61
Author(s):  
O. I. Kalchenko ◽  
A. V. Solovyov ◽  
J. Lipkowski ◽  
V. I. Kalchenko
Keyword(s):  
Stability Constants ◽  
Water Solution ◽  
Reversed Phase ◽  
Benzene Derivatives ◽  
Reversed Phase Hplc ◽  
Guest Molecules ◽  
Hplc Study

Stability constants of the host–guest complexes of 5,17-bis( N-tolyliminomethyl)-25,27-dipropoxycalix[4]arene with benzene derivatives were determined by reversed-phase HPLC in acetonitrile–water solution.

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