Method Development for Separation of Active Ingredients in Cold Medicines by Micellar Electrokinetic Chromatography

Separation of nine commonly used active ingredients in cold medicines, were demonstrated by micellar electrokinetic chromatography. The ingredients included paracetamol, chlorpheniramine maleate, diphenhydramine hydrochloride, triprolidine hydrochloride, phenylpropanolamine hydrochloride, dextromethorphan hydrobromide, loratadine, aspirin and caffeine. Effects of buffer concentrations, pH, organic modifiers and capillary length were investigated. The optimum conditions were achieved in 10 mM sodium dihydrogenphosphate-sodium tetraborate buffer, pH 9.0, containing 50 mM sodium dodecyl sulfate and 28% v/v acetonitrile using the effective length of 50 cm, the separating voltage of +15 kV and the capillary temperature of 30°C. Separation of all peaks was obtained within 28.4 min with a resolution of 1.2.

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Analysis of Seven Biogenic Amines and Two Amino Acids in Wines Using Micellar Electrokinetic Chromatography

Applied Sciences ◽

10.3390/app9061193 ◽

2019 ◽

Vol 9 (6) ◽

pp. 1193 ◽

Cited By ~ 3

Author(s):

Chih-Ying Huang ◽

Yu-Xian Wang ◽

Xin-Zhi Wang ◽

Cho-Chun Hu ◽

Tai-Chia Chiu

Keyword(s):

Amino Acids ◽

Biogenic Amines ◽

Micellar Electrokinetic Chromatography ◽

Low Cost ◽

Background Electrolyte ◽

Sodium Dodecyl ◽

Fast Method ◽

Electrokinetic Chromatography ◽

Optimal Separation

A low-cost, simple, and fast method utilizing micellar electrokinetic chromatography for the simultaneous determination of seven biogenic amines and two amino acids was developed. A background electrolyte containing 5 mM phosphate buffer (pH 3.7) and 20 mM sodium dodecyl sulfate was used. The optimal separation of nine investigated analytes was achieved in 11 min, with limits of detection (S/N = 3) ranging from 0.11 to 0.61 µM. The linear ranges for all analytes were observed between 0.55 and 10.0 μM (R2 > 0.990). The developed approach was extended to the analysis of analytes in commercial wine and beer samples. The recoveries of the proposed method ranged from 98.8% to 115.6%.

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Determination of Regularly Distributed Plant Protectants in Raw and Drinking Waters, Using a Multiresidue Method with Cyclodextrin-Modified Micellar Electrokinetic Chromatography

Journal of AOAC International ◽

10.1093/jaoac/82.6.1510 ◽

1999 ◽

Vol 82 (6) ◽

pp. 1510-1522 ◽

Cited By ~ 3

Author(s):

Hanno Stutz ◽

Hans Malissa

Keyword(s):

Environmental Protection Agency ◽

Micellar Electrokinetic Chromatography ◽

Solid Phase ◽

Tap Water ◽

Sodium Dodecyl ◽

Electrokinetic Chromatography ◽

The European Union ◽

Multiresidue Method ◽

Drinking Waters ◽

Wide Range

Abstract Eighteen plant protectant compounds were separated and determined by cyclodextrin-modified micellar electrokinetic chromatography (MEKC) in a multiclass/multiresidue method. The pesticides included are those dispersed in the greatest amounts today over agricultural acreage, and they represent 8 different classes of compounds (azoles, benzoic acids, chloroacetanilides, phenoxy acids, phenylureas, sulfonylureas, thiocarbamates, and triazines) covering a wide range of chemical reactivities and physicochemical properties. A 500 mL sample of tap water is preconcentrated by solid-phase extraction (SPE) with 300 mg combined polystyrene-divinylbenzene and methacrylate macroporous resins. Trapped analytes are eluted collectively with diethyl ether. Concentration and solvent change yield 250 μL of an acetone “concentrate,” which is further worked up and concentrated 1:10 to produce the MEKC injection solution containing 10 mmol/L sodium dodecyl sulfate (SDS) surfactant. For MEKC,2 phosphate/SDS buffer systems were designed, each allowing complete separation of all pesticides in a single run. Sensitivity was enhanced by a self-etched bubble cell and an injection procedure which employs stacking at reversed polarity. The ability of MEKC to determine plant protectants in raw and drinking waters at the 0.1 μg/L level, as demanded by the guidelines of the European Union, was demonstrated with spiked tap waters. Recoveries were between 75 and 110%, and limits of quantification, evaluated as method detection limits according to guidelines of the U.S. Environmental Protection Agency, ranged between 0.03 and 0.10 μg/L. The precisions of the relative migration times were all below 0.5%.

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