Scanning Electron Microscopic Surface Study of Adhesive organ of Garra gotyla gotyla (Gray)

2017 ◽  
Vol 6 (5) ◽  
pp. 178-185
Author(s):  
M. S. Sharma ◽  
K. C. Sharma

An attempt to study the adhesive organ of Garra gotyla gotyla inhabiting hill streams of Aravalli region has been made using scanning electron micro-scope. The results were compared with that of scanning electron microscop-ic studies of the adhesive organ of G. gotyla gotyla of the Himalayan region. The SEM study reveals that the upper fringed lip (UFL)and the lower lip or the posterior free labial fold (PFLF) bears short stub shaped tubercles (ST). These tubercles in turn bear numerous spines (S). The tubercles are covered with squamous epithelium (SE) indicating that spines are modified squamous epithelial cells. The margins of stub-shaped tubercles bear smaller spines; their size increases from margin to the centre. The surface ultrastructure of the adhesive disc of G. gotyla gotyla revealed the presence of hexagonal epithelial cells with elevated cell boundaries. Each spine is attached to callous pad with a broad base. The spines are used for anchorage to the substratum so that fish can scrap food even in fast current of water. Tubercles are used by fish for protection against mechanical injuries. Further these may be taxo-nomically used for conspecific recognition. Cumulative action of tubercles and mucus enables the fish to make firm hold on the substratum.

2011 ◽  
Vol 12 (6) ◽  
pp. 463-468 ◽  
Author(s):  
Manjiri Thakur ◽  
Vinay Hazare

ABSTRACT Aim Aim of the study is to study the surface epithelial cells of erosive, nonerosive lichen planus by scanning electron microscope (SEM) and to correlate it with that of normal mucosa and oral leukoplakia. Materials and methods Twenty biopsies of lichen planus and five biopsies of normal mucosa were taken. Half piece of each biopsy was processed for H&E stain and another half for SEM study. Result Surface epithelial cells were showing different features. Conclusion Definite different features were seen in lichen planus-leukoplakia group than in normal oral mucosa. These changes are due to abnormality in cell maturation and differentiation. Clinical significance SEM study is helpful in early detection of dysplasia. It can lead to early treatment of the lesion and thus prevention of squamous cell carcinoma. How to cite this article Thakur M, Hazare V. Scanning Electron Microscopic Study of Surface Epithelial Cells in Erosive and Nonerosive Oral Lichen Planus. J Contemp Dent Pract 2011; 12(6):463-468.


2000 ◽  
Vol 52 (5) ◽  
pp. 445-453 ◽  
Author(s):  
E.-N. Emmanouil-Nikoloussp ◽  
M. Goret-Nicaise ◽  
Ch. Foroglou ◽  
E. Katsarma ◽  
A. Dhem ◽  
...  

1997 ◽  
Vol 3 (S2) ◽  
pp. 113-114
Author(s):  
C. Baker ◽  
E.D. Green

The large eggs of Haematopinus phacochoeri are found attached to the long dorsal mane bristles of the diurnal warthog. The eggs are generally exposed to great environmental stresses such as excessive sunlight and temperature as well as the occasional plunge under water or mud during grooming. This investigation was therefore undertaken to determine how the eggs of H. phococoeri are adapted to tolerate the various environmental changes it is constantly exposed to. To our knowledge no other SEM study on the eggs of this species has been performed.Specimens still attached to the bristles were fixed in 70% ethanol, dehydrated and ultrasonically cleaned. Some eggs were longitudinally sectioned with a razor blade to expose the chorionic layers. The eggs were critical point dried in liquid CO2, mounted and viewed by means of a Leica Stereoscan 420 scanning electron microscope.The general structure of the shell consists of two layers of chorion, i.e. the endo-and exochorion. These two layers are attached at the pre-formed line of weakness at the operculum and again near the hydropyle. Elsewhere these two layers are completely separated by the respiratory layer.


1978 ◽  
Vol 15 (3) ◽  
pp. 417-427 ◽  
Author(s):  
J. Pohlenz ◽  
W. J. Bemrick ◽  
H. W. Moon ◽  
N. F. Cheville

Transmission and scanning electron microscopy of the ileal mucosae from 12 calves infected with Cryptosporidium sp. showed cryptosporidia free in the lumen and attached to epithelium. The attached parasites were interpreted to be extracellular and adherent to the microvillous border of epithelial cells. Stages of the organism included crescentic-free merozoites, trophozoites, schizonts, gametes and oocysts. Attached parasites were detected chiefly at villous tips and all stages were present on a single villus. Attachment sites were characterized by absence or disintegration of microvilli, disorganization of the terminal web and development of a specialized attachment zone. There were increased numbers of lysosomes and irregularities in the nuclear membrane of parasitized epithelial cells. It was concluded that cryptosporidia exist in bovine intestine as extracellular parasites and cause epithelial changes that in turn probably cause or contribute to diarrheal disease.


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