Effects of Reduced- Protein Diets at Constant Total Sulfur Amino Acids: Lysine Ratio on Pullet Development and Subsequent Laying Hen Performance

Abstract Total sulfur amino acids (TSAA): Lys level and Met source effects on growth performance and oxidative status were evaluated in nursery pigs fed oxidized soybean-oil. A total of 240 weaned pigs (BW=5.84± 0.96kg) were blocked by BW and randomly allotted to 60 pens. Treatments included a positive control containing fresh soybean-oil (PC) and 4 peroxidized soybean-oil treatments in a 2×2 factorial of 2 Met sources (DL-Met vs. 2-hydroxy-4-(methylthio)butanoate [HMTBa]) and 2 standardized ileal digestible TSAA: Lys levels (L1=0.58 vs. L2=0.65). Diets were fed for 42d. One pig per pen was euthanized at d42 for tissue analyses. Data were analyzed using PROC MIXED procedure of SAS. TSAA: Lys ratio did not affect ADG and ADFI. Met source did not affect ADG. HMTBa tended to increase ADFI compared to DL-Met (P = 0.08). There was a tendency for interaction between TSAA: Lys level and Met source for G: F (P = 0.07); pigs fed HMTBa-L1 tended to have decreased G: F compared to HMTBa-L2 and DL-Met-L1-L2. HMTBa had increased liver total antioxidant status (TAS) compared to DL-Met (P = 0.02). There was no TSAA:Lys level effect on TAS. Comparing PC to peroxidized treatments: pigs fed HMTBa-L1 and DL-Met-L1 tended to have decreased ADG (P ≤ 0.10) and DL-Met-L2 had decreased ADG (P < 0.05) than PC. HMTBa-L2 was similar to PC. Pigs fed DL-Met-L1 had decreased (P < 0.05) and DL-Met-L2 tended to have decreased ADFI (P ≤ 0.10) than PC. HMTBa-L1-L2 were similar to PC. All peroxidized treatments had similar G:F compared to PC. Pigs fed DL-Met-L2 tended to have decreased TAS compared to PC (P ≤ 0.10), but PC was similar to pigs fed DL-Met-L1 and HMTBa-L1-L2. In conclusion, under an oxidative challenge, HMTBa, at a TSAA: Lys ratio of 0.65, maintained nursery pigs’ growth performance and oxidative status.

Download Full-text

Estimation of the total sulfur amino acid requirement and the effect of betaine in diets deficient in total sulfur amino acids for the weanling pig.

Journal of Animal Science ◽

10.2527/2001.7961557x ◽

2001 ◽

Vol 79 (6) ◽

pp. 1557 ◽

Cited By ~ 14

Author(s):

J O Matthews ◽

L L Southern ◽

T D Bidner

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Sulfur Amino Acid ◽

Total Sulfur ◽

Sulfur Amino Acids ◽

Amino Acid Requirement

Download Full-text

The effect of dietary protein and sulfur amino acids on hepatic glutathione concentration and glutathione-dependent enzyme activities in the rat

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y88-171 ◽

1988 ◽

Vol 66 (8) ◽

pp. 1048-1052 ◽

Cited By ~ 31

Author(s):

P. F. Bauman ◽

T. K. Smith ◽

T. M. Bray

Keyword(s):

Amino Acids ◽

Glutathione Peroxidase ◽

Glutathione Reductase ◽

Dietary Protein ◽

Transferase Activity ◽

Sulfur Amino Acids ◽

Glutathione S Transferase ◽

Glutathione Concentration ◽

Hepatic Glutathione ◽

Protein Diets

Hepatic glutathione concentration and glutathione-dependent enzymes, glutathione S-transferase, glutathione peroxidase, and glutathione reductase, are important for protection against toxic compounds. Rats were fed diets containing 4, 7.5, 15, or 45% protein for 2 weeks. Glutathione and cysteine concentrations in rats fed the 4 and 7.5% protein diets were significantly lower (p < 0.05) than in rats fed the 15 and 45% protein diets. Glutathione S-transferase activity increased with increasing dietary protein. Glutathione peroxidase activity was significantly lower (p < 0.05) in rats fed 4 and 7.5% protein compared with rats fed 15 and 45% protein, whereas the activity of glutathione reductase was higher in rats fed 4 and 7.5% protein then in rats fed 15 or 45% protein. Dietary sulfur amino acids alone could account for the increase in glutathione concentration resulting from the increase in dietary protein from 7.5 to 15%. The limited availability of glutathione in animals fed the low protein diets could reduce the potential for detoxification of xenobiotics.

Download Full-text