Impacts of Age, Genotype and Feeding Low-Protein Diets on the N-Balance Parameters of Fattening Pigs

The effects of feeding low-protein (LP) diets and the age and genotype of fattening pigs were evaluated in an N-balance trial. Sixty weaned piglets of two genotypes were allotted to three different diets. Besides the control diets for the crossbred Topigs 20 × DanBred Duroc (TD) and Hungarian Large White (HLW) pigs, two LP diets were fed containing 1.5 (T1.5) and 3% (T3) less dietary protein than the control. The LP diets were supplemented with crystalline lysine, threonine, tryptophan, and methionine to equalize their digestible amino acid contents. Starter diets were fed between 20–30, grower I between 30–40, grower II between 40–80 and finisher between 80–110 kg live weights. Pigs were kept in floor pens, with 10 animals per pen. In all phases, six pigs with similar live weight were placed into individual balance cages and in the frame of a seven-day long balance trial, the daily N-intake, fecal and urinary N-excretion were measured. From the data N-digestibility, the total ammoniacal nitrogen (TAN) and N-retention were calculated. All the investigated main factors, the genotype and age of pigs and the protein content of the diets had significant effects on the N-balance of fattening pigs. The determinacy of the factors depended on the investigated parameter. Fecal N-excretion and N-digestibility were steadier compared with the urinary N-exertion and TAN percentage. N-digestibility increased and the urinary N-excretion decreased when LP diets were fed. The urinary N-decreasing effect of LP diets was not linear. Compared with the control (19.6 gN/day), T1.5 treatment resulted in 14.5, treatment T3 in 12.4 g daily urinary N-excretion. The TAN and the N-retention of HLW pigs were more favorable than those of TD pigs. Based on our results, it can be concluded that the accuracy of the nitrogen and TAN excretion values of pigs, used in the calculation of the national NH3 inventories, could be improved if the genotype, the more detailed age categories and the different protein levels of feeds are considered.

Effects of Dietary Protein Levels on Fecal Amino Acids Excretion and Apparent Digestibility, and Fecal and Ileal Microbial Amino Acids Composition in Weaned Piglets

Background and Aims: The purpose of this study was to determine the effects of low protein diets with the same Lys, Met + Cys, Thr, and Trp levels as in high protein diets on the fecal amino acid excretion and apparent digestibility, and ileal and fecal microbial amino acids composition in weaned piglets.Methods: Fifty-four 21-day-old Duroc × Landrace × Yorkshire weaned piglets were randomly divided into three groups and fed with corn-soybean meal basal diets, in which the crude protein (CP) content was 20% (H-CP), 17% (M-CP), and 14% (L-CP), respectively. The experiment included a 7-day adaptation period and a 45-day trial period. Six piglets in each group were randomly slaughtered on days 10, 25, and 45 of the trial period, and the intestinal contents, intestinal mucosa, and feces were collected.Results: The results showed that the interaction between feeding time and dietary CP levels was reflected in the apparent digestibility of dietary CP and amino acid (AA) (p < 0.01). With the increase of age, the apparent digestibility of CP and AA were increased (p < 0.01). With the increase of CP levels, the excretion of nitrogen (N) was decreased (p < 0.01), whereas the flow of microbial AA in the ileum and feces were increased (p < 0.01). The interaction between feeding time and dietary CP levels was also reflected in the composition of AA in the ileum and stool of piglets (p < 0.01). The proportion of His, Lyr, Met, Cys, and Ser was lower than the average, whereas the proportion of Phe, Leu, Pro, Ala, Glu, and Asp was higher than the average. With the increase of age, the AA content of microorganisms increased (p < 0.01).Conclusion: All in all, this work revealed the changes of N, CP, and AA excretion and digestibility of feces and microorganisms of piglets under the combined action of different dietary protein levels and different feeding times, and also the changes of AA composition of intestinal microorganisms and AA composition of microorganisms.

Effects of supplementing low-protein diets with sodium dichloroacetate and glucose on growth performance, carcass traits and meat quality of growing-finishing pigs

The objective of this study was to evaluate the effects of supplementing low-protein diets with sodium dichloroacetate (DCA) and glucose on growth performance, carcass traits, and meat quality of growing-finishing pigs. A total of 80 crossbred (Duroc × Landrace × Large White) growing barrows (27 ± 0.4 kg body weight) were allocated randomly to one of the five treatments during three successive 4-week periods. There were five diets in each phase. Diet 1 was the control diet with normal protein levels (CON) where protein levels in the three phases were 18%, 16.5%, and 15.5%, respectively. The dietary protein levels of Diets 2, 3, 4, and 5 (the low-protein diets, LP) were decreased by 4.5% compared to Diet 1. Additionally, Diets 3 and 4 were supplemented with an extra 120 mg/kg DCA (LP + DCA) or 1.8% glucose (LP + GLUC), respectively. Diet 5 was further supplemented with an extra 120 mg/kg DCA and 1.8% glucose (LP + DCA + GLUC). The LP + DCA diet increased the average daily weight gain of pigs compared to the CON and LP diet in phase 3 and the overall experimental period (P < 0.001). The LP diet reduced the gain:feed ratios of the pigs compared to the CON, LP + DCA, and LP + DCA + GLUC diets in phase 1 and the overall experimental period (P < 0.001). Furthermore, gain:feed ratios in LP + DCA and LP + DCA + GLUC groups did not differ from that of the CON group (P > 0.10). Pigs fed the LP + DCA diet had higher pH values of meat at 24 h post-mortem than the CON group (P < 0.05). The LP + DCA + GLUC diet increased the total protein content in the longissimus dorsi (LD) muscle of pigs, compared to the other dietary treatments (P < 0.05), and increased the Arg and Leu contents in the LD muscle compared to the LP + DCA diet (P < 0.05). Moreover, the LP + DCA diet induced a higher C18:1n9t percentage in the LD muscle of pigs compared to other groups (P < 0.05). In conclusion, an LP diet reduced the feed efficiency in pigs and barely affected meat quality, whereas 120 mg/kg DCA supplementation in an LP diet improved the growth performance of growing-finishing pigs, showed modest effects on carcass traits, and improved the muscle protein content with the addition of glucose.