scholarly journals Hepatitis C virus genotype and its correlation with viral load in patients from Kathmandu, Nepal

2020 ◽  
Vol 14 (12) ◽  
pp. 1470-1474
Author(s):  
Bhavesh Kumar Mishra ◽  
Uday Narayan Yadav ◽  
Saroj Khatiwada ◽  
Man Kumar Tamang ◽  
Shivir Dahal ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Viral Load ◽  
Hepatitis C ◽  
High Viral Load ◽  
Hcv Rna ◽  
Virus Genotype ◽  
Median Viral Load ◽  
Hcv Genotype ◽  
Hcv Genotypes ◽  
Genotype 3A

Introduction: Knowledge about the distribution of hepatitis C virus (HCV) genotype and its correlation with viral load are important for the decision of treatment and the prediction of disease progression, however such information is very limited in Nepal. Here, we investigated the distribution of HCV genotypes and viral load for HCV-infected patients from Kathmandu, Nepal. Methodology: Ninety-six patients with HCV infection and not on antiviral therapy were enrolled from three different medical centers in Kathmandu valley, Nepal. Demographics were recorded and blood samples were collected. Plasma was separated and HCV RNA was extracted. Reverse transcriptase PCR (RT-PCR) was performed to measure the viral load, and virus genotype was determined. Results: Genotype 3a (n = 53, 55.2%) was the most prevalent, followed by 1b (n = 19, 19.8%), 1a (n = 18, 18.8%), 5a (n = 3, 3.1%), and mix types (n = 3, 3.1%). The median viral load for HCV genotype 1a was 770,942 IU/mL (IQR, 215,268-3,720,075), 1b was 700,000 IU/mL (IQR, 431,560-919,000), 3a was 1,060,000 IU/mL (IQR, 641,050-6,063,500), 5a was 673,400 IU/mL, and mixed was 6,428,000 IU/mL. A correlation between genotype and viral load was observed (p = 0.02), of which genotype 3a showed a high viral load. Conclusions: HCV genotypes 1a, 1b, 3a, and 5a were identified in Kathmandu, Nepal, and mixed genotype patients were observed in the patients studied. HCV genotype showed a correlation with viral load in patient plasma. This finding may contribute to the treatment and prevention of hepatitis C in Kathmandu, Nepal.

Sustained Negativity for HCV-RNA over 24 or More Months by Long-Term Interferon Therapy Correlates with Eradication of HCV in Patients with Hepatitis C Virus Genotype 1b and High Viral Load

Intervirology ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Yasuji Arase ◽  
Fumitaka Suzuki ◽  
Akihito Tsubota ◽  
Yoshiyuki Suzuki ◽  
Satoshi Saitoh ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Viral Load ◽  
Hepatitis C ◽  
Interferon Therapy ◽  
High Viral Load ◽  
Hcv Rna ◽  
Virus Genotype ◽  
Genotype 1B

scholarly journals Distribution of hepatitis C virus genotypes and subtypes in a group of patients with chronic hepatitis C from Canton Sarajevo, 2012-2018

2019 ◽  
Vol 49 ◽  
Author(s):  
Irma Salimović- Bešić ◽  
Adna Kahriman ◽  
Suzana Arapčić ◽  
Amela Dedeić- Ljubović
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Hcv Rna ◽  
Genotype 3 ◽  
Hcv Genotype ◽  
Hcv Genotypes ◽  
Number Of Patients ◽  
Subtype 1B

Background: Hepatitis C virus (HCV) genotypes and subtypes exhibit significant geographic variations.Aim: To analyse the distribution of genotypes/subtypes of HCV in a group of patients with chronic hepatitis C from Canton Sarajevo during 2012-2018.Material and methods:The study enrolled 247 human plasma samples of HCV-RNA positive patients with available results of HCV genotyping test.Results: During 2012-2018, the domination of subtypes 1a (34.01%), 1b (28.34%) and genotype 3 (23.89%) was registered. In 2012 and 2013, HCV subtype 1a was the most common (27/63; 42.86% and 17/40; 42.50%, respectively). In 2014, the leading HCV genotype/subtype were 3 and 1b (17/57; 29.82%). In 2015, the dominance of HCV genotype 3 (14/39; 35.90%) continued, while in 2016, the same number of HCV subtypes 1a and 1b (11/30; 36.67%) was recorded. Although in a small number of tested, during 2017, HCV subtype 1b was the most prevalent (7/14; 50.00%), and in 2018, it was replaced by a HCV subtype 1a (3/4; 75.00%). Distribution of HCV genotypes/subtypes by age group of patients varied significantly (p=0.000). The largest number of patients (71/247; 28.74%) belonged to the age category 30-39 years and HCV genotypes/subtypes 1, 3, 4, 1a and 1b were identified. Except in 2017, male gender significantly dominated (p=0.000). In males, HCV subtype 1a (68/170; 40.00%) was the most common, while in women it was HCV subtype 1b (44/77; 57.14%).Conclusion: This six-year retrospective study showed the time variations of the circulating HCV genotypes/subtypes among patients with chronic hepatitis C in Canton Sarajevo. Genotyping of the HCV has an important implications for diagnosis and treatment of the patients.

Virological Response in Patients with??Hepatitis C Virus Genotype 1b and??a??High Viral Load

2008 ◽  
Vol 28 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Gotaro Yamada ◽  
Shiro Iino ◽  
Tadao Okuno ◽  
Masao Omata ◽  
Kendo Kiyosawa ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Viral Load ◽  
Hepatitis C ◽  
Virological Response ◽  
High Viral Load ◽  
Virus Genotype ◽  
Genotype 1B

scholarly journals Efficacy of Peginterferon plus Ribavirin Treatment in Turkish Patients Infected by Hepatitis C Virus Genotype 1: Comparison of Low and High Viral Load

ANKEM Dergisi ◽  
2011 ◽  
Vol 25 (3) ◽  
pp. 164-168
Author(s):  
Ali Ilgin Olut ◽  
Alpay Ari ◽  
Onur Ozgenc ◽  
Meltem Avci ◽  
Sibel Ozsu Caymaz
Keyword(s):  
Hepatitis C Virus ◽  
Viral Load ◽  
Hepatitis C ◽  
High Viral Load ◽  
Genotype 1 ◽  
Virus Genotype ◽  
Turkish Patients

scholarly journals Investigating the Origin and Spread of Hepatitis C Virus Genotype 5a

2006 ◽  
Vol 80 (9) ◽  
pp. 4220-4226 ◽  
Author(s):  
Jannick Verbeeck ◽  
Piet Maes ◽  
Philippe Lemey ◽  
Oliver G. Pybus ◽  
Elke Wollants ◽  
...  
Keyword(s):  
South Africa ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
South African ◽  
Virus Spread ◽  
Virus Genotype ◽  
Hcv Genotype ◽  
Hcv Genotypes ◽  
Rate Of Spread ◽  
Phylogenetic Studies

ABSTRACT Epidemiological and phylogenetic studies of hepatitis C virus (HCV) have identified six major HCV genotypes and have attempted to characterize their origin and spread worldwide. Putative regions of endemic infection have been identified for all HCV genotypes except HCV genotype 5a. Although HCV genotype 5a was previously thought to be largely restricted to the northern part of South Africa, this study reports an unexpected cluster of the genotype in West Flanders Province in Belgium. To investigate the molecular epidemiology of this cluster and of HCV genotype 5a in general, a rigorous phylogenetic analysis of Belgian and South African HCV genotype 5a samples was performed. Remarkably, the Belgian and South African strains form two distinct clusters of similar diversity. We used a Bayesian coalescent method to estimate the rate of virus spread through time for HCV genotype 5a in both regions. Our results indicate that HCV genotype 5a strains have been spreading independently in Belgium and South Africa for more than 100 years, with a rate of spread characteristic of an epidemic genotype. These findings have major implications for tracing the origin of HCV genotype 5a. Here, we speculate about the possible origins of these clusters.

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