scholarly journals Methylprednisolone inhibits the proliferation and affects the differentiation of rat spinal cord-derived neural progenitor cells cultured in low oxygen conditions by inhibiting HIF-1α and Hes1 in vitro

2014 ◽  
Vol 34 (3) ◽  
pp. 788-795 ◽  
Author(s):  
WENHAO WANG ◽  
PENG WANG ◽  
SHIYUAN LI ◽  
JIEWEN YANG ◽  
XINJUN LIANG ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Low Oxygen ◽  
Rat Spinal Cord ◽  
Oxygen Conditions ◽  
Cells Cultured

scholarly journals Chondroitinase ABC Treatment and the Phenotype of Neural Progenitor Cells Isolated From Injured Rat Spinal Cord

2011 ◽  
pp. 705-708 ◽  
Author(s):  
L. SLOVINSKÁ ◽  
I. NOVOTNÁ ◽  
D. ČÍŽKOVÁ
Keyword(s):  
Spinal Cord ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Cns Injury ◽  
Rat Spinal Cord ◽  
Chondroitinase Abc ◽  
Differentiation Potential ◽  
Cord Injury ◽  
The Impact

The aim of the present study was to investigate whether enzyme chondroitinase ABC (ChABC) treatment influences the phenotype of neural progenitor cells (NPCs) derived from injured rat spinal cord. Adult as well as fetal spinal cords contain a pool of endogenous neural progenitors cells, which play a key role in the neuroregenerative processes following spinal cord injury (SCI) and hold particular promise for therapeutic approaches in CNS injury or neurodegenerative disorders. In our study we used in vitro model to demonstrate the differentiation potential of NPCs isolated from adult rat spinal cord after SCI, treated with ChABC. The intrathecal delivery of ChABC (10 U/ml) was performed at day 1 and 2 after SCI. The present findings indicate that the impact of SCI resulted in a decrease of all NPCs phenotypes and the ChABC treatment, on the contrary, caused an opposite effect.

Impact of chemokines on the properties of spinal cord-derived neural progenitor cells in a rat spinal cord lesion model

2014 ◽  
Vol 93 (4) ◽  
pp. 562-571 ◽  
Author(s):  
Friederike Knerlich-Lukoschus ◽  
Sebastian Krossa ◽  
Jörg Krause ◽  
H. Maximilian Mehdorn ◽  
Axel Scheidig ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Spinal Cord Lesion ◽  
Rat Spinal Cord ◽  
Cord Lesion

scholarly journals Low oxygen alters mitochondrial function and response to oxidative stress in human neural progenitor cells

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e1486 ◽  
Author(s):  
Yury M. Lages ◽  
Juliana M. Nascimento ◽  
Gabriela A. Lemos ◽  
Antonio Galina ◽  
Leda R. Castilho ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neuronal Development ◽  
Neural Progenitor ◽  
Low Oxygen ◽  
Atp Production ◽  
Oxygen Levels ◽  
Human Neural Progenitor Cells

Oxygen concentration should be carefully regulated in all living tissues, beginning at the early embryonic stages. Unbalances in oxygen regulation can lead to cell death and disease. However, to date, few studies have investigated the consequences of variations in oxygen levels for fetal-like cells. Therefore, in the present work, human neural progenitor cells (NPCs) derived from pluripotent stem cells grown in 3% oxygen (v/v) were compared with NPCs cultured in 21% (v/v) oxygen. Low oxygen concentrations altered the mitochondrial content and oxidative functions of the cells, which led to improved ATP production, while reducing generation of reactive oxygen species (ROS). NPCs cultured in both conditions showed no differences in proliferation and glucose metabolism. Furthermore, antioxidant enzymatic activity was not altered in NPCs cultured in 3% oxygen under normal conditions, however, when exposed to external agents known to induce oxidative stress, greater susceptibility to DNA damage was observed. Our findings indicate that the management of oxygen levels should be considered forin vitromodels of neuronal development and drug screening.

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