scholarly journals CARACTERIZAÇÃO FÍSICO-QUÍMICA E MICROBIOLÓGICA DA CARNE DO MOLUSCO BIVALVE SARNAMBI (Phacoides pectinitus) COLETADO NAS PRAIAS EM ALGODOAL E SALINÓPOLIS, NO PARÁ

Author(s):  
Priscila Brasil Dias Salles ◽  
Yuri Bertolo Macedo ◽  
Elaine Lopes Figueiredo

O Sarnambí (Lucina pectinata) é um molusco muito encontrado em praias paraenses, e possui grande aceitabilidade por parte de seus consumidores. No entanto, poucas pesquisas foram realizadas visando conhecer suas caracterisiticas físico-quimicas e higiênico-sanitárias  Assim, este trabalho objetivou avaliar as caracterisiticas físico-químicas e microbiologica da carne do sarnambí coletada na praia da Lua Cheia, localizada na Ilha de Algodoal (Amostra A) e praia do Paraíso na Vila de Cuiarana, município de Salinópolis (Amostras B), no Estado do Pará, e realizar um levantamento sobre o método de extração da carne deste molusco. Foram determinados os niveis de umidade, proteínas, lipídeos, colesterol, resíduo mineral fixo, carboidrato, valor calórico, pH, bases nitrogenadas voláteis totais, e minerais (ferro, cobre, magnésio, manganês, zinco e potássio) para a caracterização físico-química. As análises microbiologicas foram: contagem padrão de aeróbias mesófilas, contagem de fungos filamentosos e leveduras, Coliformes Totais e Termotolerantes, Staphylococcus aureus, pesquisa de Salmonella e Clostridim Sulfito Redutores. Os resultados das análises físico-química demonstraram que este alimento é considerado uma fonte expressiva de proteína, e baixos índices de lipídeos e valor calórico. Não houve contaminação por Staphylococcus aureus, Salmonella e Clostridium Sulfito Redutores em nenhuma das amostras avaliadas, o que garante dizer que as mesmas encontram-se dentro dos padrões de qualidade e de acordo com a legislação vigente. Entretanto, houve contaminação em todas as amostras avaliadas de bactérias aeróbias mesófilas, fungos filamentosos e leveduras, e Coliformes Totais e Termotolerantes, confirmando a falta de cuidados higiênico-sanitários dos manipuladores e dos utensílios envolvidos no beneficiamento do alimento.

Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).


Author(s):  
Margaret Hukee

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.


VASA ◽  
2013 ◽  
Vol 42 (5) ◽  
pp. 382-386
Author(s):  
Karim Gariani ◽  
Marc Righini ◽  
Marco Roffi ◽  
Gino Gemayel ◽  
Damiano Mugnai ◽  
...  

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