scholarly journals Karyotype and COI gene sequence of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia

2021 ◽  
Vol 15 (4) ◽  
pp. 447-458
Author(s):  
Viktor V. Bolshakov ◽  
Alexander A. Prokin ◽  
Sergey V. Artemenko
Keyword(s):  
Phylogenetic Tree ◽  
Gene Sequence ◽  
Genetic Distances ◽  
Coi Gene ◽  
Gene Sequences ◽  
South Caucasus ◽  
Homozygous State ◽  
Banding Sequence ◽  
First Time ◽  
Georgian Population

The karyotype features and gene COI sequence of Chironomus heteropilicornis Wülker, 1996 from the Gydan Peninsula are presented for the first time. Nine banding sequences were determined, eight of them hpiA2, hpiB1, hpiC1, hpiC2, hpiD1, hpiE1, hpiF3 and hpiG1 were previously known from European, Georgian (South Caucasus) and Siberian populations. One new banding sequence for Ch. heteropilicornis, hpiB2, was found. The hpiA2 banding sequence was found in all individuals, and this is its second finding after the Georgian population (Karmokov 2019). The hpiF3 banding sequence was found only in the homozygous state. Additional B-chromosomes are absent. The genetic distances (K2P) between Ch. heteropilicornis COI gene sequence from Gydan Peninsula and Norway are 1.1­–1.3%, and Georgia – 1.8%, much lower than the commonly accepted threshold of 3% for species of genus Chironomus Meigen, 1803. The phylogenetic tree for COI gene sequences estimated by Bayesian inference showed geographically determined clusters of Norway and Gydan and a separate lineage of the Georgian population of Ch. heteropilicornis. The analysis of karyotype and COI gene sequences shows that the population of Ch. heteropilicornis from the Gydan Peninsula has an intermediate position within the Ch. pilicornis group between Georgian, Yakutian and Norwegian populations. The position of Ch. pilicornis Fabricius, 1787 from Canada and Greenland on the phylogenetic tree is discussed.

scholarly journals Karyotype characteristics, chromosomal polymorphism and gene COI sequences of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the South Caucasus

2019 ◽  
Vol 13 (4) ◽  
pp. 339-357
Author(s):  
Mukhamed Kh. Karmokov
Keyword(s):  
Chromosomal Polymorphism ◽  
Genetic Distances ◽  
Caucasian Population ◽  
Coi Gene ◽  
The South ◽  
South Caucasus ◽  
Gene Coi ◽  
Banding Sequence ◽  
Separate Line ◽  
Coi Sequences

The study presents data on the karyotype characteristics, features of chromosomal polymorphism and the gene COI sequences of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the South Caucasus. We found 8 banding sequences in the Caucasian population. Overall, The Caucasian population of the species can be characterized as having a low level of polymorphism. We found one new banding sequence hpiA2 in the banding sequence pool of Ch. heteropilicornis. We observed inversion polymorphism only in the arm F. The dendrogram of genetic distances by Nei criteria (1972) shows a clear separation of the Caucasian population from populations of Siberia. At the same time, the distance between populations of Siberia and the population of South Caucasus (0.379–0.445) almost reach the mean distance (0.474 ± 0.314) between subspecies (Gunderina 2001). Due to this, we can assume that the population of South Caucasus separated from Siberian populations at the level of subspecies. Constructed on data for COI gene sequences the phylogenetic tree estimated by the Bayesian inference shows that the sequences of Ch. heteropilicornis from the South Caucasus form a separate line in the general branch of Ch. heteropilicornis sequences. At the same time, calculated K2P genetic distances between Ch. heteropilicornis sequences from Norway and Caucasus (2.0–2.2%) do not exceed the 3% threshold for the genus Chironomus.

scholarly journals Integrative Taxonomy and Molecular Phylogeny of the Plant-Parasitic Nematode Genus Paratylenchus (Nematoda: Paratylenchinae): Linking Species with Molecular Barcodes

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 408
Author(s):  
Phougeishangbam Rolish Singh ◽  
Gerrit Karssen ◽  
Marjolein Couvreur ◽  
Sergei A. Subbotin ◽  
Wim Bert
Keyword(s):  
18S Rrna ◽  
Parasitic Nematode ◽  
Integrative Taxonomy ◽  
Coi Gene ◽  
Integrated Analysis ◽  
Gene Sequences ◽  
Unknown Species ◽  
New Gene ◽  
First Time ◽  
Plant Parasitic

Pin nematodes of the genus Paratylenchus are obligate ectoparasites of a wide variety of plants that are distributed worldwide. In this study, individual morphologically vouchered nematode specimens of fourteen Paratylenchus species, including P. aculentus, P. elachistus, P. goodeyi, P. holdemani, P. idalimus, P. microdorus, P. nanus, P. neoamblycephalus, P. straeleni and P. veruculatus, are unequivocally linked to the D2-D3 of 28S, ITS, 18S rRNA and COI gene sequences. Combined with scanning electron microscopy and a molecular analysis of an additional nine known and thirteen unknown species originating from diverse geographic regions, a total of 92 D2-D3 of 28S, 41 ITS, 57 18S rRNA and 111 COI new gene sequences are presented. Paratylenchus elachistus, P. holdemani and P. neoamblycephalus are recorded for the first time in Belgium and P. idalimus for the first time in Europe. Paratylenchus is an excellent example of an incredibly diverse yet morphologically minimalistic plant-parasitic genus, and this study provides an integrated analysis of all available data, including coalescence-based molecular species delimitation, resulting in an updated Paratylenchus phylogeny and the corrective reassignment of 18 D2-D3 of 28S, 3 ITS, 3 18S rRNA and 25 COI gene sequences that were previously unidentified or incorrectly classified.

scholarly journals New records of Celaenorrhinus pyrrha de Nicéville, 1889 and C. munda (Moore, 1884) from China (Lepidoptera, Hesperiidae)

ZooKeys ◽  
2020 ◽  
Vol 985 ◽  
pp. 61-70
Author(s):  
Guo-Xi Xue ◽  
Yutaka Inayoshi ◽  
Meng Li ◽  
Fu-Ming Zhang ◽  
Da-Kun Lai ◽  
...  
Keyword(s):  
Gene Sequence ◽  
New Records ◽  
Morphological Characters ◽  
Coi Gene ◽  
Distribution Map ◽  
Male Specimen ◽  
Autonomous Region ◽  
Tibet Autonomous Region ◽  
First Time ◽  
The Tibet Autonomous Region

Celaenorrhinus pyrrha de Nicéville, 1889, a rare species of Hesperiidae previously known to be distributed from northeastern India to Indochina, is reported from southwestern Yunnan and southwestern Chongqing, China. A 658 bp COI gene sequence of this species is published for the first time. Although Chongqing is obviously isolated from the main distribution range, morphological characters of the specimens from this locality do not indicate a subspecies differentiation. Another rare taxon, C. munda munda (Moore, 1884), is also recorded from China for the first time based upon a male specimen from Cuona County in the Tibet Autonomous Region. This is the second specimen of C. munda from China, over 100 years after the holotype of C. munda joka Evans, 1949. The genitalia of both species are illustrated and described. Some taxonomic notes and a distribution map are provided as well.

Redefinition of Cornexcisia Fan & Kano, 2000 (Diptera: Sarcophagidae), with the first description of the female of Sarcophaga kurahashii (Shinonaga & Tumrasvin, 1979)

Zootaxa ◽  
2019 ◽  
Vol 4668 (3) ◽  
pp. 410-420
Author(s):  
CHAO WANG ◽  
YUNYUN GAO ◽  
THOMAS PAPE ◽  
DONG ZHANG
Keyword(s):  
Cytochrome Oxidase ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Gene Sequences ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Senior Synonym ◽  
First Time ◽  
Mitochondrial Cytochrome Oxidase

Sarcophaga Meigen, 1826 is proposed as a senior synonym of Cornexcisia Fan & Kano, 2000, syn. nov. and Fanzideia Xue, Verves & Du, 2011, syn. nov. Cornexcisia Fan & Kano, 2000, stat. rev. is given status as a subgenus and is considered a senior synonym of Fanzideia Xue, Verves & Du, 2011, syn. nov. at the subgeneric level. Cornexcisia is argued to contain S. (Cornexcisia) longicornuta (Fan & Kano, 2000), comb. nov., S. (C.) cygnocerca (Xue, Verves & Du, 2011), comb. nov., S. (C.) kurahashii (Shinonaga & Tumrasvin, 1979), subgen. comb. nov. (from Phallosphaera Rohdendorf) and S. (C.) suthep Pape & Bänziger, 2003, subgen. comb. nov. (from Rosellea Rohdendorf). Sarcophaga (C.) kurahashii is newly recorded from China (Yunnan), the male is redescribed and the female is described for the first time, supported by photographs, illustrations and mitochondrial cytochrome oxidase subunit I (COI) gene sequences. Species of Cornexcisia share an exceptionally long postpedicel in the female and the following apomorphic distiphallic appendages in the male: juxta ventro-proximally with an apically divided arm with cuticular pile, and lateral styli bifurcated from the base with each branch elongate, gently curved and slightly expanded apically. A key to the species of Cornexcisia is provided. 

scholarly journals DNA Barcoding of Fish in Mischief Reef—Fish Diversity of a Reef Fish Community From Nansha Islands

2021 ◽  
Vol 7 ◽  
Author(s):  
Binbin Shan ◽  
Yan Liu ◽  
Changping Yang ◽  
Yu Zhao ◽  
Gongjun Zhang ◽  
...  
Keyword(s):  
Reef Fish ◽  
Management Strategies ◽  
Genetic Distances ◽  
Coi Gene ◽  
Fish Diversity ◽  
Nansha Islands ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
First Time ◽  
Effective Conservation

Development of effective conservation and management strategies requires assessments of ecosystem biodiversity status, especially in understudied hotspots of global fish diversity. Coral reefs are important habitats for fishes, with biodiversity hotspots known globally. We present the first data on molecular diversity of fishes of Mischief Reef, the largest atoll in the Nansha Islands. Partial sequences (650 bp) of mitochondrial COI gene (Cytochrome c oxidase subunit I) are used to identify 209 individuals, representing 101 species, referable to 62 genera, 27 families, 8 orders, and 1 class. The most abundant orders are the Perciformes (176 specimens, 84.21%), Tetraodontiformes (13 specimens, 6.22%), and Beryciformes (13 specimens, 6.22%). Mean Kimura 2-Parameter genetic distances within genera, families, and orders are 4.51, 13.90, and 17.63%, respectively. We record Monotaxis heterodon from this region for the first time—a species that may previously have been misidentified as M. grandoculis. In addition, we recognized possible cryptic species of Lethrinus olivaceus based on significantly diverging barcode sequences. Barcode data provide new insights into fish diversity of Mischief Reef, important for developing further researches on this fauna, and for its conservation.

scholarly journals Genetic identification of Javan hawk-eagle (Nisaetus bartelsi) from Indonesia using mitochondrial COI gene

2019 ◽  
pp. 70-77
Author(s):  
Suhadi ◽  
Dwi Listyorini ◽  
Riri Wiyanti Retnaningtyas ◽  
Fima Rizki Eka Putri ◽  
Dina Ayu Valentiningrum
Keyword(s):  
Phylogenetic Tree ◽  
Gene Sequence ◽  
Dna Isolation ◽  
Genetic Identification ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Conservation Effort ◽  
Interspecific Distance ◽  
Intraspecific Distance

Nisaetus bartelsi is a native hawk from Java which its genetic information is not commonly understood yet. As a part of the conservation effort to maintain genetic diversity of this endangered species, this research aimed to obtain COI gene sequences from five individuals of N. bartelsi to confirm its position in the phylogenetic tree. DNA isolation from 5 N. bartelsi blood sample was performed and its COI gene sequence was amplified, sequenced, and used to reconstruct phylogenetic tree using MEGA6 with several other members of Nisaetus, Aquila, and Saggitaridae family. Furthermore, the intraspecific distance between 5 N. bartelsi samples and interspecific distance with other species were calculated using MEGA6. The result suggested that all five individuals belonged to the species Javan hawk-eagle (N. bartelsi) and were closely related to the Blyth’s hawk-eagle (Nisaetus alboniger). The DNA barcoding of the Javan hawk-eagle conducted in this study is a stepping stone to conservation efforts for the Javan hawk-eagle.

scholarly journals Karyotype and COI sequences of Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera,Chironomidae) from the bay of Orkhon River, Mongolia

2021 ◽  
Vol 15 (2) ◽  
pp. 149-157
Author(s):  
Viktor V. Bolshakov ◽  
Alexander A. Prokin
Keyword(s):  
Bayesian Inference ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Dna Barcoding ◽  
Molecular Genetic ◽  
Species Level ◽  
Coi Gene ◽  
High Similarity ◽  
Coi Sequences ◽  
First Time

Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera, Chironomidae) is recorded from Mongolia for the first time. Eleven banding sequences determined in the Mongolian population were previously known from Altai and Yenisei populations: sokA1, sokB1, sokB2, sokC1, sokC2, sokD1, sokD2, sokE1, sokF1, sokF2 and sokG1. The additional B-chromosomes are absent. DNA-barcoding of COI gene was carried out for this species for the first time. The phylogenetic tree estimated by Bayesian inference showed a high similarity of the studied species with Ch. acutiventris Wülker, Ryser et Scholl, 1983 from the Chironomus obtusidens-group. The estimated genetic distance K2P between Ch. sokolovae and Ch. acutiventris is much lower (0.38%) than the commonly accepted threshold of 3% for species of genus Chironomus Meigen, 1803. Our results show that the accepted cytogenetic criteria of species level in the genus Chironomus are more in accordance with morphological ones of the same level, than with molecular-genetic criteria accepted for species COI genetic distance.

scholarly journals Introduced Prophysaon andersonii (J.G. Cooper, 1872) in Quebec, Canada: first record of Prophysaon (Gastropoda, Eupulmonata, Arionoidea) in eastern North America, confirmed by partial-COI gene sequence

Check List ◽  
2020 ◽  
Vol 16 (2) ◽  
pp. 307-316
Author(s):  
Annegret Nicolai ◽  
Robert G. Forsyth
Keyword(s):  
North America ◽  
New Record ◽  
Gene Sequence ◽  
Sequence Data ◽  
First Record ◽  
Coi Gene ◽  
Eastern North America ◽  
Western North America ◽  
Native Range ◽  
First Time

We report for the first time the terrestrial slug Prophysaon andersonii (J.G. Cooper, 1872) from Quebec, Canada. Two specimens were collected in Parc national du Bic. The identification was determined by the external morphology and partial-COI gene sequence data. The genus Prophysaon is endemic to western North America, and the new record indisputably represents an introduction. No species of Prophysaon has, until now, been noticed in North America from outside its native range.

scholarly journals Molecular classification and comparative phylogeographic study of insectivorous bat species (Pipisitrellus coromandra) from Punjab, Pakistan

2022 ◽  
Vol 82 ◽  
Author(s):  
Z. Ahmad ◽  
M. H. Abbasi ◽  
S. R. Ahmad ◽  
N. Sheikh
Keyword(s):  
Molecular Classification ◽  
Genetic Distances ◽  
Coi Gene ◽  
Local Alignment ◽  
Identity Match ◽  
Gene Coi ◽  
Search Tool ◽  
Identity Score ◽  
First Time

Abstract Molecular based identification of bat fauna in Pakistan has been relatively less explored. The current study was therefore planned to report for the first time the molecular classification of insectivorous bats (Pipistrellus coromandra) based on mitochondrion gene (COI) from Punjab, Pakistan. Specimens were collected from five different locations followed by DNA extraction with subsequent gene amplification and sequencing. All samples in the study had shown close identity matches with species (Pipistrellus coromandra) from India and (Pipistrellus tenuis) from Vietnam with percentage identity score of 96.11 and 95.58 respectively except one sequence which only revealed 86.78% identity match on Basic Local Alignment Search Tool (BLAST) and could only be assigned to genus level Pipistrellus sp. The results indicated negligible intra-population genetic distance among collected samples whereas the comparison with species from other countries had shown high intraspecific (P. coromandra) and interspecific (P. tenuis) mean genetic distances. The current study hence successfully proved the efficiency of COI gene as a molecular marker for species identification and in analyzing the patterns of genetic variation with species from other countries.

Molecular and morphological characterisation of Rhabdias picardiae Junker, Lhermitte-Vallarino et Bain, 2010 (Nematoda: Rhabdiasidae) from Delaland’s River Frog, Amietia delalandii (Duméril et Bibron, 1841) (Amphibia: Pyxicephalidae) in South Africa

2018 ◽  
Vol 63 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Roman Svitin ◽  
Yuriy Kuzmin ◽  
Louis du Preez
Keyword(s):  
Body Length ◽  
Gene Sequence ◽  
Buccal Capsule ◽  
Original Description ◽  
Coi Gene ◽  
Morphological Comparison ◽  
New Host ◽  
Infective Larvae ◽  
Tail Tip ◽  
First Time

Abstract Rhabdias picardiae previously known from the Guttural Toad, Sclerophrys gutturalis (Bufonidae) is described from a new host, Delaland’s River Frog, Amietia delalandii (Pyxicephalidae). The species identification is confirmed by analysis of the COI gene sequence and morphological comparison with the original description. Morphological details of apical structures in adult parasitic R. picardiae, namely, the shape and position of lips, the number and position of external labial papillae, and the buccal capsule morphology are described using light and scanning electron microscopy. The buccal capsule width and length, the distance from anterior end to nerve ring and excretory pore, and ratios of distance to vulva to body length and body length to width were identified as less variable metrical characters in the studied sample of 30 specimens. Adult specimens demonstrated differences in the position of lips in relation to the oral opening and position of the buccal capsule in relation to the oesophagus depending on the size (age) of worms. The infective larvae of R. picardiae are described for the first time and characterised by two lateral alae consisting of two ridges, triangular pseudolabia with rounded tops, and presence of ornamentation on the rounded tail tip.

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