scholarly journals DNA Barcoding of Fish in Mischief Reef—Fish Diversity of a Reef Fish Community From Nansha Islands

2021 ◽  
Vol 7 ◽  
Author(s):  
Binbin Shan ◽  
Yan Liu ◽  
Changping Yang ◽  
Yu Zhao ◽  
Gongjun Zhang ◽  
...  
Keyword(s):  
Reef Fish ◽  
Management Strategies ◽  
Genetic Distances ◽  
Coi Gene ◽  
Fish Diversity ◽  
Nansha Islands ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
First Time ◽  
Effective Conservation

Development of effective conservation and management strategies requires assessments of ecosystem biodiversity status, especially in understudied hotspots of global fish diversity. Coral reefs are important habitats for fishes, with biodiversity hotspots known globally. We present the first data on molecular diversity of fishes of Mischief Reef, the largest atoll in the Nansha Islands. Partial sequences (650 bp) of mitochondrial COI gene (Cytochrome c oxidase subunit I) are used to identify 209 individuals, representing 101 species, referable to 62 genera, 27 families, 8 orders, and 1 class. The most abundant orders are the Perciformes (176 specimens, 84.21%), Tetraodontiformes (13 specimens, 6.22%), and Beryciformes (13 specimens, 6.22%). Mean Kimura 2-Parameter genetic distances within genera, families, and orders are 4.51, 13.90, and 17.63%, respectively. We record Monotaxis heterodon from this region for the first time—a species that may previously have been misidentified as M. grandoculis. In addition, we recognized possible cryptic species of Lethrinus olivaceus based on significantly diverging barcode sequences. Barcode data provide new insights into fish diversity of Mischief Reef, important for developing further researches on this fauna, and for its conservation.

scholarly journals Integrative description of two new species of Malagasy chirping giant pill-millipedes, genus Sphaeromimus (Diplopoda: Sphaerotheriida: Arthrosphaeridae)

2017 ◽  
Author(s):  
Leif Moritz ◽  
Thomas Wesener
Keyword(s):  
New Species ◽  
Coi Gene ◽  
Micro Ct ◽  
Mitochondrial Coi ◽  
Taxonomic Description ◽  
Mitochondrial Coi Gene ◽  
Two New Species ◽  
Ct Analysis ◽  
Ct Technology ◽  
First Time

The species-rich giant pill-millipedes (Sphaerotheriida) often represent a microendemic component of Madagascar’s mega-invertebrate fauna. Of the chirping genus Sphaeromimus de Saussure & Zehntner, 1902, ten species have been described. Here, we describe two new species of Sphaeromimus integratively, combining light microscopy, scanning electron microscopy, DNA barcoding and micro-CT technology for the first time in a taxonomic description of a giant pill-millipede. S. kalambatritra sp. nov. and S. midongy sp. nov. are the first giant pill-millipedes collected and described from the mountainous rainforests of Kalambatritra and Midongy. Both species show island gigantism compared to their congeners. Our analysis of the mitochondrial COI gene shows that the two species are related to one another with a moderate genetic distance (9.4%), while they are more closely related to an undetermined specimen from the forest of Vevembe (6.3% and 8.4%). They stand in a basal position with S. ivohibe Wesener, 2014 and S. musicus (de Saussure & Zehntner, 1897). The four aforementioned species share a high number of stridulation ribs on the male harp. Our micro-CT analysis provides a look into the head of S. kalambatritra sp. nov. and shows that non-destructive CT methods are a useful tool for studying the inner morphology of giant pill-millipedes.

Discovery of the genus Claraeola Aczél in Iran with the description of two new species (Diptera: Pipunculidae)

Zootaxa ◽  
2017 ◽  
Vol 4227 (4) ◽  
pp. 563 ◽  
Author(s):  
BEHNAM MOTAMEDINIA ◽  
CHRISTIAN KEHLMAIER ◽  
AZIZOLLAH MOKHTARI ◽  
EHSAN RAKHSHANI ◽  
EBRAHIM GILASIAN
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Identification Key ◽  
Coi Gene ◽  
Palaearctic Species ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Two New Species ◽  
Western Palaearctic ◽  
First Time

The genus Claraeola Aczél is recorded from Iran for the first time. Two new species, Claraeola parnianae Motamedinia & Kehlmaier sp. nov. and Claraeola khorshidae Motamedinia & Kehlmaier sp. nov., are described and illustrated. An updated identification key to the Western Palaearctic species of the genus Claraeola is provided. Both species were characterized morphologically and by DNA barcoding of the mitochondrial COI gene. 

Pairing of two highly sexually dimorphic species within the genus Lophocampa Harris, 1841 (Lepidoptera: Erebidae: Arctiini)

Zootaxa ◽  
2021 ◽  
Vol 4927 (1) ◽  
pp. 104-112
Author(s):  
MICHEL LAGUERRE ◽  
BENOIT VINCENTCENT
Keyword(s):  
Dna Barcode ◽  
Original Description ◽  
Coi Gene ◽  
Sexually Dimorphic ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
First Time ◽  
Dimorphic Species

Two Lophocampa species previously known only by females are paired with morphologically highly different male specimens. This pairing was initiated after the discovery of a mosaic gynandromorph specimen and then confirmed using the mitochondrial COI gene (the so-called DNA barcode). Following the discovery of a labeling error by Rothschild during the original description of two species, two recombinations are proposed. Pairs for each species are illustrated and the male specimens are described for the first time. 

First report of incidence of eriophyid mite Aceria sp. on Amaranthus

ENTOMON ◽  
2020 ◽  
Vol 44 (4) ◽  
pp. 309-310
Author(s):  
K. M. Sreekumar ◽  
N. Srinivasa ◽  
R. Sivamoorthy
Keyword(s):  
Foliar Application ◽  
Nuclear Gene ◽  
Coi Gene ◽  
Species Delineation ◽  
Amaranthus Tricolor ◽  
Eriophyid Mite ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
First Time ◽  
Damage Symptoms

Eriophyid mite Aceria sp. damaging Amaranthus (Amaranthus tricolor) is reported from India for the first time. The mite cause severe malformation of the shoot, making it fibrous and reducing the yield. Foliar application of spiromesifen or fenpyroximate reduced the damage symptoms. DNA data for the mitochondrial COI gene and nuclear gene (ITS 2 region) are being generated for accurate species delineation.

Morphological description and DNA barcoding of Hydrobaenus golovinensis sp. nov. (Diptera: Chironomidae: Orthocladiinae) from the Russian Far East

Zootaxa ◽  
2017 ◽  
Vol 4286 (2) ◽  
pp. 277
Author(s):  
EUGENYI A. MAKARCHENKO ◽  
MARINA A. MAKARCHENKO ◽  
ALEXANDER A. SEMENCHENKO ◽  
OLEG A. VELIAEV
Keyword(s):  
Dna Barcoding ◽  
Russian Far East ◽  
Far East ◽  
Instar Larva ◽  
Genetic Distances ◽  
Coi Gene ◽  
Morphological Description ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
The Russian Far East

Illustrated descriptions of adult male, pupa and fourth instar larva, as well as DNA barcoding results of Hydrobaenus golovinensis sp. nov. in comparison with closely related species H. majus Makarchenko et Makarchenko and H. sikhotealinensis Makarchenko et Makarchenko from the Russian Far East are provided. Partial mitochondrial COI gene (DNA barcoding) of the new species has been sequenced and uploaded to GenBank. Comparisons with corresponding regions of COI between H. golovinensis and other species in the genus produce K2P genetic distances of 10.3–14.3%, the values well above those associated with intraspecific variation. In contrast, genetic distances among 18 specimens are all within the range of 0–3.5%. The ML tree is also constructed using DNA barcodes obtained in the present study and those of other species of Hydrobaenus Fries from GenBank. 

Spiophanes norrisi sp. nov. (Polychaeta: Spionidae)—a new species from the NE Pacific coast, separated from the Spiophanes bombyx complex based on both morphological and genetic studies

Zootaxa ◽  
2009 ◽  
Vol 2278 (1) ◽  
pp. 1-25 ◽  
Author(s):  
KARIN MEIßNER ◽  
MIRIAM BLANK
Keyword(s):  
Species Complex ◽  
Genetic Distances ◽  
Coi Gene ◽  
Genetic Studies ◽  
Complex Species ◽  
Mitochondrial Coi ◽  
Body Regions ◽  
Mitochondrial Coi Gene ◽  
Morphological Differences ◽  
Ne Pacific

Morphological and genetic studies of specimens identified as Spiophanes bombyx (Claparède, 1870) revealed that the supposedly cosmopolitan species represents a species complex. Species belonging to this complex are S. bombyx (Claparède, 1870), S. aucklandicus Meißner, 2005, S. uschakowi Zachs, 1933 and the newly described S. norrisi. Morphological differences between these species are slight and concern the start of sabre chaetae, the shape of dorsal ciliated organs, and the start and number of neuropodial hooks in relation to body size. Specimens from the Atlantic Ocean examined in the course of this study also belong to this species complex but could not be assigned to any of the known species. Genetic differences between S. bombyx and S. norrisi in both examined genes (18S, COI) are significant and genetic distances are comparable to distances found between sister species in other spionid taxa. As expected, the nuclear 18S rRNA is generally more conserved than the mitochondrial COI gene. The significance beyond taxonomy of dorsal ciliated organs and of the presence of sabre chaetae in certain body regions is briefly discussed, but conclusions about the phylogenetic relationships and character evolution within Spiophanes must remain tentative.

A review of genus Agriocnemis larva (Odonata: Coenagrionidae) from Thailand including a description of the final stadium larva of Agriocnemis minima Selys, 1877 with supporting molecular (COI) data

Zootaxa ◽  
2019 ◽  
Vol 4711 (3) ◽  
pp. 579-599
Author(s):  
TOSAPHOL SAETUNG ◽  
BOONSATIEN BOONSOONG
Keyword(s):  
Dna Barcoding ◽  
Species Identification ◽  
Ventral View ◽  
Coi Gene ◽  
Compound Eyes ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
First Time

The larva of Agriocnemis minima Selys, 1879 is described and illustrated for the first time, based on reared specimens collected from Thailand. Selected larvae of Agrioncnemis Selys, 1877 were matched with their adults by DNA barcoding. The mitochondrial COI gene (658 bp) of three species (A. minima, A. femina femina (Brauer, 1868), and A. pygmaea (Rambur, 1842)) occurring in Thailand was analysed to confirm the species identification and to determine the association between the larva and adult stages. The larva of A. minima can be distinguished from known species by the following combination of characteristics: 1) long simple setae on the antennomeres I and II, 2) protrusion of the male cerci as long as 0.5× the S10, and 3) tufts of spiniform setae on the lateral occiput margin and on the ventral view of the compound eyes. Comparisons to known larvae of Agriocnemis and those of some other subfamily Agriocnemidinae members are also provided. 

A review of the genus Forficuloecus Conci (Phthiraptera: Philopteridae) from parrots (Psittaciformes: Psittacidae), with descriptions of four new species

Zootaxa ◽  
2008 ◽  
Vol 1859 (1) ◽  
pp. 49 ◽  
Author(s):  
ROGER D. PRICE ◽  
KEVIN P. JOHNSON ◽  
RICARDO L. PALMA
Keyword(s):  
New Species ◽  
Coi Gene ◽  
Chewing Louse ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Genetic Distinctiveness

Ten species, including four new species, of the chewing louse genus Forficuloecus Conci, 1941, are recognized from Australasian parrots, and a key is given for their identification. The new species and their type hosts are: F. cameroni ex the Red-winged Parrot, Aprosmictus erythropterus (J.F. Gmelin, 1788); F. banksi ex the Mulga Parrot, Psephotus varius Clark, 1910; F. wilsoni ex the Northern Rosella, Platycercus venustus (Kuhl, 1820); and F. josephi ex the Bourke's Parrot, Neopsephotus bourkii (Gould, 1841). Partial sequences of the mitochondrial COI gene support the genetic distinctiveness of these new species.

Are Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara (Acari: Tetranychidae) one species? Morphological and molecular evidences for synonymy between these two spider mite species and a note on invasiveness of T. macfarlanei on okra and eggplant in India

2017 ◽  
Vol 22 (4) ◽  
pp. 467
Author(s):  
Mahran Zeity ◽  
Nagappa Srinivas ◽  
Chinnamade Channegowde Gowda
Keyword(s):  
Spider Mite ◽  
Morphological Characters ◽  
Molecular Data ◽  
Mite Species ◽  
Coi Gene ◽  
High Similarity ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
High Degree ◽  
Degree Of Similarity

Study of morphological characters of Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara revealed high similarity by comparing all the important characters in addition to the characters pointed out by Ehara to separate those two species. Molecular phylogeny of seven Indian populations of T. macfarlanei and one population of T. malaysiensis from Philippines along with few distantly related species of Tetranychus was attempted. High degree of similarity between these two species at mitochondrial COI gene (96%) as well as ITS2 (rDNA) (96–99%) region was evident. Based on both morphological features and molecular data, T. malaysiensis is proposed as a junior synonym of T. macfarlanei based on ICZN’s law of priority. Also more female characters are prompted in this study to distinctly discriminate T. macfarlanei from its most resembling species, Tetranychus ludeni Zacher. Tetranychus macfarlanei has emerged as a pest of several cultivated crop plants in India. 

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