scholarly journals New species of Retiboletus (Boletales, Boletaceae) from China based on morphological and molecular data

MycoKeys ◽  
2020 ◽  
Vol 67 ◽  
pp. 33-44
Author(s):  
Hai-Ying Liu ◽  
Yan-Chun Li ◽  
Tolgor Bau
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Large Subunit ◽  
Elongation Factor ◽  
Molecular Data ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Line Drawings ◽  
Morphological And Molecular Data ◽  
Elongation Factor 1

Species of the genus Retiboletus in China were investigated based on morphology and phylogenetic analysis of DNA sequences from the nuclear ribosomal large subunit (nrLSU) and the translation elongation factor 1-α gene (TEF1-α). Nine species were recovered from China, including two new and seven known species. The new species, namely Retiboletus ater and R. sinogriseus, from southwestern and northeastern China respectively, are documented and illustrated in this paper. Retiboletus ater is morphologically characterized by its black to grayish black pileus, white to grayish hymenophore, black to blackish stipe and white to grayish white context. Retiboletus sinogriseus is morphologically characterized by its brown to grayish-brown pileus, yellow to grayish-yellow hymenophore, pale yellow to brownish stipe and yellow to brownish-yellow context. Descriptions and line drawings of these two novel species and their comparisons with allied taxa are presented.

Type studies on two Paxillus species (Paxillaceae, Boletales) described from China

Phytotaxa ◽  
2022 ◽  
Vol 530 (2) ◽  
pp. 177-188
Author(s):  
LI-RONG LIU ◽  
GENG-SHEN WANG ◽  
LIU-KUN JIA ◽  
JU-QING KANG ◽  
ZHU-LIANG YANG ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Dna Sequences ◽  
Large Subunit ◽  
Elongation Factor ◽  
Translation Elongation ◽  
Line Drawings ◽  
Molecular Phylogenetic ◽  
Type Studies ◽  
Elongation Factor 1 ◽  
Molecular Phylogenetic Data

Types and recently collected samples of two Paxillus species namely P. rhytidophyllus and P. yunnanensis, originally described from southwestern China, were critically restudied based on morphology and molecular phylogenetic data of DNA sequences from the large subunit of the nuclear ribosomal RNA (nrLSU), the nuclear ribosomal internal transcribed spacer (ITS), and the translation elongation factor 1-α (tef1-α). The results showed that these two species belong to Boletinellus and Tricholomopsis, respectively. Thus, two new combinations, Boletinellus rhytidophyllus and Tricholomopsis yunnanensis are proposed. Boletinellus rhytidophyllus is characterized by a deeply decurrent and shallow hymenophore which is poroid-lamellate to alveolate, slightly thick-walled (0.6–1 μm) basidiospores, occasionally 2- to 4-spored basidia, rare or infrequent hymenial cystidia, and a trichodermal pileipellis. Tricholomopsis yunnanensis is characterized by a convex pileus densely covered by red-violet to red-brown fibrillose squamules, a yellowish stipe sparsely covered with red to red-brown fibrillose squamules, subglobose to broadly ellipsoid basidiospores, prominent large cheilocystidia measuring 60–195 × 11–39 μm, and a palisadic pileipellis. New descriptions and line drawings of these two species and their comparisons with allied taxa are presented.

scholarly journals A morphological and molecular survey of Neoconidiobolus reveals a new species and two new combinations

2021 ◽  
Author(s):  
Yong Nie ◽  
Zi-Min Wang ◽  
Xiao-Yong Liu ◽  
Bo Huang
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Elongation Factor ◽  
Small Subunit ◽  
New Combinations ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1 ◽  
A New Species

Abstract The genus Neoconidiobolus was recently established to accommodate all members of the Conidiobolus subgenus Conidiobolus. Based on mitochondrial small subunit (mtSSU), nuclear large subunit (nucLSU) of rDNA and translation elongation factor 1-alpha (TEF1), this study further resolved the genus Neoconidiobolus into three clades, with three new taxa being added. They are N. kunyushanensis B. Huang & Y. Nie, sp. nov., N. lamprauges (Drechsler) B. Huang & Y. Nie, comb. nov., and N. nanodes (Drechsler) B. Huang & Y. Nie, comb. nov. Meanwhile, a morphologial comparison among species in the three clades and a key to the species of the genus Neoconidiobolus are provided herein.

Cantharellus macrocarpus (Cantharellaceae, Cantharellales), a new species from tropical China

Phytotaxa ◽  
2021 ◽  
Vol 484 (2) ◽  
pp. 170-180
Author(s):  
YU-ZHUO ZHANG ◽  
ZHI-QUN LIANG ◽  
HUI-JING XIE ◽  
LU-LING WU ◽  
ROU XUE ◽  
...  
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Elongation Factor ◽  
Translation Elongation ◽  
Tropical Asia ◽  
Line Drawings ◽  
Yellow Orange ◽  
Tropical China ◽  
Elongation Factor 1 ◽  
A New Species

Cantharellus macrocarpus, a member of Cantharellus subgenus Cantharellus, is described as a new species from Hainan Province, China. It is characterized by large basidioma with a bright yellow-orange pileus and a well-developed, rugulose hymenophore, cream to pale yellowish stipe, noticeably thickened hyphal walls in the pileipellis, presence of clamp connections in all parts of the basidiomata, and a distribution in tropical Asia. A phylogenetic analysis of DNA sequences from part of the 28S gene and the translation elongation factor 1-a gene (TEF1) confirmed that it forms an independent lineage within subgenus Cantharellus. A detailed description, color photos of fresh basidiomata and line drawings of micromorphological features are presented.

Amanita griseofusca: A new species of Amanita in section Vaginatae from Malam Jabba, Swat, Pakistan

Phytotaxa ◽  
2018 ◽  
Vol 364 (2) ◽  
pp. 181 ◽  
Author(s):  
MUNAZZA KIRAN ◽  
JUNAID KHAN ◽  
HASSAN SHER ◽  
DONALD H. PFISTER ◽  
ABDUL NASIR KHALID
Keyword(s):  
New Species ◽  
Elongation Factor ◽  
Molecular Data ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Pileus Surface ◽  
Elongation Factor 1 ◽  
A New Species ◽  
Partial Translation

A new species, Amanita griseofusca in section Vaginatae is described and illustrated here from Pakistan. Distinguishing characters of the new species include medium-sized basidiomata, greyish brown pileus surface with white to beige, membranous volval remnants present as one (large) to a few (small) warts, close lamellae which are cream colored with a pink tone, striations one third of the total pileus radius, broadly ellipsoidal to ellipsoidal basidiospores and white loose saccate volva turning beige at maturity. Molecular data inferred from partial nuc rDNA internal transcribed spacer region (ITS), partial nuc rDNA larger subunit region (LSU) and partial translation elongation factor 1-alpha (tef1) confirms the novelty of the present taxon.

Baorangia duplicatopora (Boletaceae, Boletales), a new bolete from tropical China

Phytotaxa ◽  
2021 ◽  
Vol 508 (1) ◽  
Author(s):  
XU ZHANG ◽  
ZHI-QUN LIANG ◽  
SHUAI JIANG ◽  
CHANG XU ◽  
XIN-HUA FU ◽  
...  
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Its Region ◽  
Tropical Region ◽  
Translation Elongation ◽  
Line Drawings ◽  
Microscopic Features ◽  
Tropical China

Baorangia duplicatopora is described as a new species from Hainan Province, a tropical region of China. It is morphologically characterized by large to very large basidiomata with a dull rose red, rose pink to purplish red pileus, compound pores, pileus context near hymenophore and stipe context staining blue when injured, a red stipe, and cheilocystidia wider than those of other Baorangia species. Phylogenetic analyses of DNA sequences from part of the 28S gene, the nuc rDNA internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α gene (TEF1) also confirmed that B. duplicatopora forms an independent lineage within Baorangia. Detailed descriptions, color photographs of fresh basidiomata, and line drawings of microscopic features of the new species are presented. A key to species of Baorangia in the world is also provided.

Morphological characters and phylogenetic analysis reveal a new species of Phellinus with hooked hymenial setae from Vietnam

Phytotaxa ◽  
2018 ◽  
Vol 356 (1) ◽  
pp. 91 ◽  
Author(s):  
LIN ZHU ◽  
XING JI ◽  
JING SI ◽  
BAO-KAI CUI
Keyword(s):  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Molecular Data ◽  
Translation Elongation ◽  
The Status ◽  
Large Subunit Rdna ◽  
Elongation Factor 1 ◽  
A New Species

Phellinus vietnamensis sp. nov. is described from Vietnam based on morphological characters and molecular data. Morphologically, it is characterized by perennial, pileate basidiomata, a dimitic hyphal system, hooked hymenial setae, and colorless, broadly subglobose to ovoid, thick-walled basidiospores 5.5–6 × 4.8–5.2 μm. Phylogenetically, the status of Phellinus vietnamensis is strongly supported based on sequences of the nuclear internal transcribed spacer (ITS) regions, the translation elongation factor 1-α gene (EF1-α) nuclear large subunit rDNA (nrLSU) and the second largest subunits of RNA polymerase II (RPB2).

A new species of Heimioporus (Boletaceae) from southern China

Phytotaxa ◽  
2019 ◽  
Vol 415 (4) ◽  
pp. 179-188
Author(s):  
XIANG-NYU CHEN ◽  
MING ZHANG ◽  
TAI-HUI LI ◽  
NIAN-KAI ZENG
Keyword(s):  
New Species ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Molecular Data ◽  
Translation Elongation ◽  
Distinct Member ◽  
Elongation Factor 1 ◽  
A New Species

Heimioporus sinensis, collected from tropical and subtropical areas of China, is introduced as a new species based on both morphological characters and molecular data. The species is characterized by the purplish red to deep magenta pileus, the reticulated stipe, the irregularly reticulate to reticulate-alveolate basidiospores 11.5–13.5 × 8–9.5 μm, and a trichodermal to intricately trichodermal pileipellis. Phylogenetic analyses based on the nuc 28S rDNA D1-D2 domains (28S) and the translation elongation factor 1-α gene (tef1-α) showed that H. sinensis is a distinct member of the genus Heimioporus in the subfamily Xerocomoideae.

scholarly journals Four new East Asian species of Aleurodiscus with echinulate basidiospores

MycoKeys ◽  
2019 ◽  
Vol 52 ◽  
pp. 71-87
Author(s):  
Sheng-Hua Wu ◽  
Chia-Ling Wei ◽  
Yu-Ting Lin ◽  
Chiung-Chih Chang ◽  
Shuang-Hui He
Keyword(s):  
New Species ◽  
Southwest China ◽  
East Asian ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Northwest Yunnan ◽  
Elongation Factor 1 Alpha ◽  
Alpha Gene ◽  
Elongation Factor 1

Four new species of Aleurodiscus sensu lato with echinulate basidiospores are described from East Asia: A.alpinus, A.pinicola, A.senticosus, and A.sichuanensis. Aleurodiscusalpinus is from northwest Yunnan of China where it occurs on Rhododendron in montane habitats. Aleurodiscuspinicola occurs on Pinus in montane settings in Taiwan and northwest Yunnan. Aleurodiscussenticosus is from subtropical Taiwan, where it occurs on angiosperms. Aleurodiscussichuanensis is reported from southwest China on angiosperms in montane environments. Phylogenetic relationships of these four new species were inferred from analyses of a combined dataset consisting of three genetic markers, viz. 28S, nuc rDNA ITS1-5.8S-ITS2 (ITS), and a portion of the translation elongation factor 1-alpha gene, TEF1.

scholarly journals A revision of Onychelmis Hinton, 1941 (Coleoptera: Elmidae), with description of new species, DNA barcoding and notes on the geography of the genus

2021 ◽  
Vol 739 ◽  
pp. 1-35
Author(s):  
Marek Linský ◽  
Zuzana Čiamporová-Zaťovičová ◽  
Fedor Čiampor Jr
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Species Delimitation ◽  
Molecular Data ◽  
Distribution Map ◽  
Line Drawings ◽  
The Andes ◽  
Long Time ◽  
New Material ◽  
Morphological And Molecular Data

The genus Onychelmis Hinton, 1941 was for a long time regarded as a small taxon with only three known species distributed in the Andes. A study of new material from Ecuador, using morphological and molecular data, has resulted in the discovery of five new species: Onychelmis lenkae sp. nov., O. lobata sp. nov., O. minor sp. nov., O. onorei sp. nov. and O. splendida sp. nov. We also revised the entire genus and redescribed the three known species, O. longicollis (Sharp, 1882), O. leleupi Delève, 1968 and O. whiteheadi Spangler & Santiago, 1991. Habitus photographs of adults are provided, together with line drawings of male and female genitalia, and schematic illustrations of the distribution of femoral tomentum for each species. DNA sequences for barcoding the COI mtDNA fragment were used to support species delimitation and to suggest possible relationships among species. The revision includes a key to adults of all species of Onychelmis and notes on the biogeography of the genus, with an updated distribution map.

scholarly journals Four new species of Trichoderma in the Harzianum clade from northern China

MycoKeys ◽  
2020 ◽  
Vol 73 ◽  
pp. 109-132
Author(s):  
Xin Gu ◽  
Rui Wang ◽  
Quan Sun ◽  
Bing Wu ◽  
Jing-Zu Sun
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Elongation Factor ◽  
Northern China ◽  
Gene Encoding ◽  
Translation Elongation ◽  
Nuclear Rna ◽  
Encoding Gene ◽  
Elongation Factor 1 ◽  
Nuclear Rna Polymerase

The Harzianum clade of Trichoderma comprises many species, which are associated with a wide variety of substrates. In this study, four new species of Trichoderma, namely T. lentinulae, T. vermifimicola, T. xixiacum, and T. zelobreve, were encountered from a fruiting body and compost of Lentinula, soil, and vermicompost. Their colony and mycelial morphology, including features of asexual states, were described. For each species, their DNA sequences were obtained from three loci, the internal transcribed spacer (ITS) regions of the ribosomal DNA, the gene encoding the second largest nuclear RNA polymerase subunit (RPB2), the translation elongation factor 1-α encoding gene (TEF1-α). The analysis combining sequences of the three gene regions distinguished four new species in the Harzianum clade of Trichoderma. Among them, T. lentinulae and T. xixiacum clustered with T. lixii, from which these new species differ in having shorter phialides and smaller conidia. Additionally, T. lentinulae differs from T. xixiacum in forming phialides with inequilateral to a strongly-curved apex, cultural characteristics, and slow growth on PDA. Trichoderma vermifimicola is closely related to T. simmonsii, but it differs from the latter by producing phialides in verticillate whorls and smaller conidia. Trichoderma zelobreve is the sister species of T. breve but is distinguished from T. breve by producing shorter and narrower phialides, smaller conidia, and by forming concentric zones on agar plates. This study updates our knowledge of species diversity of Trichoderma.

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