scholarly journals Description of Longidorus cheni sp. n. (Nematoda, Longidoridae) from China

ZooKeys ◽  
2018 ◽  
Vol 744 ◽  
pp. 1-18 ◽  
Author(s):  
Eda Marie Barsalote ◽  
Hoa Thi Pham ◽  
Stela Lazarova ◽  
Vlada Peneva ◽  
Jingwu Zheng
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Molecular Data ◽  
Rrna Genes ◽  
28S Rrna ◽  
Oral Aperture ◽  
Asiatic Origin ◽  
18S Rrna Genes ◽  
Guide Ring ◽  
Partial 18S

Longidoruschenisp. n., an amphimictic species recovered from the rhizosphere ofLarixprincipis-rupprechtiiandPyracanthafortuneanain Shanxi and Beijing, China, is described and illustrated. The taxonomic position ofL.chenisp. n.among other species within the genus was elucidated using morphometric and molecular data, and phylogenetic relationships were inferred using D2–D3 expansion domains of 28S and 18S rRNA genes by Bayesian Inference (BI) method. The new species is characterised by females with a medium body size (L = 4.9–6.6 mm), a lip region slightly expanded, broadly rounded frontally and laterally, the amphidial fovea broad and symmetrically bilobed at base, odontostyle long and slender (143–168 μm), odonthophore slightly swollen at the base, tail short bluntly conoid to rounded. Guide ring located far posterior from the oral aperture (70–93 μm). Males with two ad-cloacal pairs of supplements preceded by a row of 10–14 ventromedian supplements, with robust spicules measuring 111–126 μm along the median line. Three juvenile stages were present, tail shape of J1 elongate conoid while in J2 and J3 the tail gradually becomes bluntly rounded. Codes for identifying the new species are: A6-B3-C5-D2-E2-F3-G1-H1-I2-J2-K2.Longidoruschenisp. n.belongs to a group of species with a guide ring at the mid-odontostyle position that have a predominantly Asiatic origin. It differs from all of them by a combination of morphological characters and unique sequences of partial 18S and D2–D3 region of 28S rRNA genes. The percentage dissimilarities in partial 18S and D2–D3 28S rRNA genes ofL.chenito the closest species (L.litchii,L.fangi,L.jonesiandL.juglans) were 1.5 %–1.8 % and 16.8–18.3 %, respectively.

Morphological and molecular characterisation of Helicotylenchus ciceri n. sp. and H. scoticus Boag & Jairajpuri, 1985 (Nematoda: Hoplolaimidae) from Iran

Nematology ◽  
2020 ◽  
Vol 22 (6) ◽  
pp. 611-626
Author(s):  
Fariba Mohammadi Zameleh ◽  
Akbar Karegar ◽  
Reza Ghaderi ◽  
Abbas Mokaram Hesar
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
18S Rrna ◽  
Phylogenetic Analyses ◽  
Rrna Genes ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Closely Related Species ◽  
Partial 18S ◽  
Molecular Characters

Summary Helicotylenchus ciceri n. sp. and H. scoticus are described and illustrated based on morphological, morphometric and molecular characters. The new species is characterised by a conical and truncated lip region with five or six distinct annuli, stylet 32-37 μm long with anteriorly concave knobs, secretory-excretory pore posterior to the pharyngo-intestinal valve, dorsally convex-conoid tail with a terminal projection, phasmids 14 (7-20) annuli anterior to the level of anus, empty spermatheca and absence of males. Intraspecific variation of 16 populations of H. scoticus, collected from chickpea and lentil fields in Kermanshah province, western Iran, is discussed. The results of the phylogenetic analyses based on the sequences of the partial 18S rRNA, D2-D3 expansion segments of 28S rRNA and ITS rRNA genes are provided for the studied species, confirming their differences from each other and determining the position of them and their relationships with closely related species.

Conflict and congruence between morphological and molecular data: revision of the Merodon constans group (Diptera : Syrphidae)

2020 ◽  
Author(s):  
Ante Vujić ◽  
Snežana Radenković ◽  
Laura Likov ◽  
Andrijana Andrić ◽  
Marina Janković ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Distinct Species ◽  
Morphological Characters ◽  
Molecular Data ◽  
Species Group ◽  
Rrna Genes ◽  
Rrna Gene ◽  
28S Rrna ◽  
The Status ◽  
Data Revision

We revise the Merodon constans species group of the genus Merodon Meigen, 1803 (Diptera: Syrphidae), provide morphological diagnosesand descriptions, as well as an illustrated key and a discussion on the different taxonomic characters used. In total, 15 species were studied, their geographic distributions are presented on maps, and nine new species are described. Two species are redefined and neotypes are designated, lectotypes are designated for five species, and onespeciesis reinstated as valid. Following a detailed study of type material in different entomological collections, the status of several species is revised and three new synonymies are proposed. The M. constans species group was resolved as being monophyletic within the M. albifrons lineage based on molecular analyses using COI and 28S rRNA gene sequences. Three species morphologically similar to M. constans (Rossi, 1794) but occurring outside its distributional rangewere supported as being valid and distinct species on the basis of molecular data, but they were not distinguishable based on morphological characters. By contrast, continental populations of M. analis Meigen, 1822 could not be separated from Mediterranean M. constans based on differences in COI or 28S rRNA genes. The same molecular markers could not discriminate between two other species pairs. We conclude that these molecular markers only partially resolve species within the M. constans group. Geometric morphometry of wing shape successfully separated M. analis and M. constans, as well as M. spineus Vujić, Šašić Zorić & Likov, sp. nov. in both species and population analyses.

A new species of the deep-sea genus Scaphander (Gastropoda, Cephalaspidea) from the Mar del Plata submarine canyon off Argentina

2020 ◽  
Vol 96 (1) ◽  
pp. 111-126
Author(s):  
Justine Siegwald ◽  
Guido Pastorino ◽  
Trond Oskars ◽  
Manuel António E Malaquias
Keyword(s):  
New Species ◽  
Deep Sea ◽  
Molecular Phylogenetics ◽  
Submarine Canyon ◽  
Molecular Data ◽  
Rrna Genes ◽  
28S Rrna ◽  
The Pacific ◽  
Morphological And Molecular Data ◽  
A New Species

Scaphander Montfort, 1810, is a genus of deep-sea, soft-bottom gastropods composed of approximately 23 species distributed worldwide. The systematics of the genus in the Atlantic was recently revised and eight species were recognized. The present study describes a new species (Scaphander meridionalis sp. n.) from the Argentine continental slope using morphological and molecular data. Shells, gizzard plates, radulae, and male reproductive systems were studied by optical and scanning electron microscopy and compared with data from all other known Scaphander species. Bayesian molecular phylogenetics based on two mitochondrial (cytochrome c oxidase subunit I and 16S rRNA) and one nuclear (28S rRNA) genes together with the molecular species delimitation method Automatic Barcode Gap Discovery were used to compare the novel samples with all known Atlantic species. Our results revealed that the Argentinian specimens have a distinct shell and penial papilla and were molecularly 6.35–6.53%, 6.9–7.44%, and 7.99–8.89% distinct (COI uncorrected p-distance) from their closest relatives, the Pacific species Scaphander grandis and Scaphander mundus, and the northern Atlantic species Scaphander nobilis, respectively. Scaphander meridionalis sp. n. is the first species only known from the South Atlantic and its occurrence off Argentina represents a new southern geographical limit of the distribution of the genus in the Atlantic Ocean.

scholarly journals Integrative taxonomy of the genus Pseudostegana (Diptera, Drosophilidae) from China, with descriptions of eleven new species

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5160 ◽  
Author(s):  
Jinming Lu ◽  
Yuan Zhang ◽  
Hongwei Chen
Keyword(s):  
New Species ◽  
Species Delimitation ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Southwestern China ◽  
28S Rrna ◽  
Nuclear Locus ◽  
Barcode Gap

The genus Pseudostegana (Okada, 1978) currently contains thirty-nine described species. A number of Pseudostegana were collected from the fieldwork in southwestern China from 2010 to 2017. Eleven new species were discovered and are described from southwestern China: Pseudostegana alpina Zhang & Chen, sp. nov.; Pseudostegana amnicola Zhang & Chen, sp. nov.; Pseudostegana amoena Zhang & Chen, sp. nov.; Pseudostegana mailangang Zhang & Chen, sp. nov.; Pseudostegana meiduo Zhang & Chen, sp. nov.; Pseudostegana meiji Zhang & Chen, sp. nov.; Pseudostegana mystica Zhang & Chen, sp. nov.; Pseudostegana stictiptrata Zhang & Chen, sp. nov.; Pseudostegana stigmatptera Zhang & Chen, sp. nov.; Pseudostegana ximalaya Zhang & Chen, sp. nov. and Pseudostegana zhuoma Zhang & Chen, sp. nov. A key to all Chinese Pseudostegana species based on morphological characters is provided. Two mitochondrial loci (COI and ND2) and one nuclear locus (28S rRNA) were sequenced for the Pseudostegana specimens, and Bayesian and RAxML concatenated analyses were run. Molecular species delimitation is performed using the distance-based automatic barcode gap discovery (ABGD) method. Molecular data support the morphological characteristics observed among these Chinese species and confirm the new species as being distinctly different.

scholarly journals Systematic position and composition of Merodon nigritarsis and M. avidus groups (Diptera, Syrphidae) with a description of four new hoverflies species

2019 ◽  
Vol 89 (1) ◽  
pp. 74-125 ◽  
Author(s):  
Laura Likov ◽  
Ante Vujić ◽  
Nataša Kočiš Tubić ◽  
Mihajla Đan ◽  
Nevena Veličković ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Systematic Position ◽  
Species Group ◽  
Rrna Genes ◽  
Coi Gene ◽  
28S Rrna ◽  
Mitochondrial Coi ◽  
Diagnostic Key ◽  
Morphological And Molecular Data

The putative monophyly and systematic position of Merodon nigritarsis group was assessed based on morphological and molecular data of the mitochondrial COI and nuclear 28S rRNA genes. The previously reported concept of the group has been redefined, and M. crassifemoris Paramonov, 1925 is now excluded. The related M. avidus group is redefined here, including the Merodon avidus complex and M. femoratus Sack, 1913. Species delimitation of morphologically defined species of M. nigritarsis group was well supported by COI gene analysis, with the exception of M. alagoezicus Paramonov, 1925 and M. lucasi Hurkmans, 1993. Descriptions are given for three new species of the M. nigritarsis species group: Merodon cohurnus Vujić, Likov et Radenković sp. n., Merodon longisetus Vujić, Radenković et Likov sp. n. and Merodon obstipus Vujić, Radenković et Likov sp. n., and one new species from the M. avidus group: Merodon rutitarsis Likov, Vujić et Radenković sp. n. A lectotype is designated for M. femoratus Sack, 1913, and two new synonymies of this species were proposed: M. biarcuatus Curran, 1939 and M. elegans Hurkmans, 1993. Here we review 18 species from the M. nigritarsis group and six species from the M. avidus group and provide morphological diagnoses of the species groups. Additionally, diagnosis of 12 branches (groups or individual taxa) of M. avidus-nigritarsis lineage, an illustrated diagnostic key for the males, and distribution map are provided for the new species.

Two new species of Scapheremaeus from Southern Japan (Acari: Oribatida: Cymbaeremaeidae), with genetic information

2018 ◽  
Vol 23 (8) ◽  
pp. 1545 ◽  
Author(s):  
Badamdorj Bayartogtokh ◽  
Shimpei F. Hiruta ◽  
Satoshi Shimano
Keyword(s):  
New Species ◽  
Eastern China ◽  
Oribatid Mites ◽  
Rrna Genes ◽  
28S Rrna ◽  
Sequence Database ◽  
Southern Japan ◽  
Two New Species ◽  
Database Comparison ◽  
18S Rrna Genes

The present paper deals with two new species of oribatid mites of the genus Scapheremaeus Berlese, 1910 from the subtropical islands in Southern Japan. Scapheremaeus nakanoshimensis sp. nov. and Scapheremaeus boninensis sp. nov. are proposed on the basis of adults. Furthermore, we present data on distribution, diversity and habitat ecology of all known species of Scapheremaeus in Japan, and a key is provided for identification of hitherto recorded species in this country. Majority of Scapheremaeus species found in Japan are known to be restricted to areas of Southern Japan and Eastern China. Only one of the species has a relatively broad distribution in South America and East Asia. Most species of Scapheremaeus in Japan are inhabitants of soil and litter of various forests, and mosses growing on rocks, but few of them are arboreal. The D3 fragments of the 28S rRNA (28S D3) and 18S rRNA genes of Scapheremaeus nakanoshimensis sp. nov. are sequenced and data are available in the sequence database. Comparison of the 28S D3 gene sequence of Scapheremaeus nakanoshimensis sp. nov. with other oribatid mites gene sequences identifies that the representatives of Scutoverticidae (Scutovertex, Provertex, Exochocepheus) and Eremaeozetidae (Eremaeozetes), Ameronothridae (Ameronothrus) and Phenopelopidae (Eupelops) are the closest relatives to Scapheremaeus with regard to the species included in this analysis. 

Description of Rotylenchus arasbaranensis n. sp. from Iran with discussion on the taxonomic status of Plesiorotylenchus Vovlas, Castillo & Lamberti, 1993 (Nematoda: Hoplolaimidae)

Nematology ◽  
2014 ◽  
Vol 16 (9) ◽  
pp. 1019-1045 ◽  
Author(s):  
Mohammad Reza Atighi ◽  
Ebrahim Pourjam ◽  
Razieh Ghaemi ◽  
Majid Pedram ◽  
Gracia Liébanas ◽  
...  
Keyword(s):  
18S Rrna ◽  
Phylogenetic Analyses ◽  
Taxonomic Status ◽  
Original Description ◽  
Molecular Data ◽  
Rrna Genes ◽  
Molecular Studies ◽  
18S Rrna Genes ◽  
First Time ◽  
Partial 18S

Rotylenchus arasbaranensis n. sp., a new monosexual species is described and illustrated based on morphological, morphometric and molecular studies. The new species is characterised by having an offset and hemispherical lip region with 5-6 annuli, 32-36 μm long stylet, vulva located at 43.9-59.2% with a single epiptygma and rounded tail, rarely bilobed, with 6-8 annuli. The species R. striaticeps and the male of R. buxophilus are reported for the first time from Iran and R. fragaricus is reported and studied for the second time after its original description. The results of the phylogenetic analyses based on the sequences of the D2-D3 expansion region of the 28S, ITS1-rRNA and the partial 18S rRNA genes were provided for the studied species, confirming their differences from each other and determining the position of them and their relationships with closely related taxa. Also, the validity of Plesiorotylenchus is discussed on the basis of molecular data and its synonymisation (with only one sequence) with Rotylenchus is accepted.

Molecular and morphological characterisation of Ditylenchus persicus n. sp. (Nematoda: Anguinidae) from Kermanshah province, western Iran

Nematology ◽  
2017 ◽  
Vol 19 (2) ◽  
pp. 211-223 ◽  
Author(s):  
Mehrab Esmaeili ◽  
Ramin Heydari ◽  
Pablo Castillo ◽  
Juan E. Palomares-Rius
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
Rrna Genes ◽  
28S Rrna ◽  
Pharyngeal Bulb ◽  
Lateral Field ◽  
Male Tail ◽  
Long Stem ◽  
18S Rrna Genes

A new species of the genusDitylenchus,D.persicusn. sp., was collected around the roots of grapevine and is described and illustrated herein based on morphological and molecular studies. The new species is characterised by a body length of 783 (635-928) μm and 689 (670-715)μm in female and male, respectively, delicate stylet 6.2 (5.0-7.0)μm long, six lines in the lateral field, median bulb of pharynx well developed, muscular with crescentic valve, basal pharyngeal bulb pyriform with 10 (8-13)μm long stem extending into intestine, post-vulval uterine sac relatively short, 16 (14-18)μm long, female and male tail elongate-conoid with finely rounded terminus, and bursa covering 65-80% of male tail length. Morphologically,D.persicusn. sp. appears closer to five known species of the genus, namely:D.arachis,D.caudatus,D.clarus,D.myceliophagusandD. nanus. The results of phylogenetic analyses based on sequences of D2-D3 expansion region of 28S rRNA, ITS and partial 18S rRNA genes confirmed the close molecular relationship betweenD.persicusn. sp. and otherDitylenchusspecies such asD. myceliophagus,D. africanus,D. arachis,D. destructorandD. halictus.

Description of Labrys fuzhouensis sp. n. and first record of Coslenchus rafiqi (Nematoda: Tylenchidae) from China

Nematology ◽  
2019 ◽  
Vol 21 (7) ◽  
pp. 693-708 ◽  
Author(s):  
Kaikai Qiao ◽  
Mengxin Bai ◽  
Yadeng He ◽  
Jingwei Chen ◽  
Shun Xiao ◽  
...  
Keyword(s):  
New Species ◽  
Tail Length ◽  
First Record ◽  
Morphological Characters ◽  
Molecular Data ◽  
Scaling Analysis ◽  
28S Rrna ◽  
New Species Description ◽  
First Time ◽  
Labial Disc

Summary In this study, we describe Labrys fuzhouensis sp. n. based on detailed morphology revealed by scanning electron microscopy and molecular data inferred from 18S and 28S rRNA. The new species differs from other Labrys species by a combination of several morphometric and morphological characters: vulva-anus distance 66-74 μm, tail length 172-182 μm, post-uterine sac 11-12 μm, excretory duct less sclerotised, and hexagon-shaped labial disc. The novelty of L. fuzhouensis sp. n. is also confirmed by multi-dimensional scaling analysis. The 18S and 28S rRNA-based phylogenies suggest that the new species nested in the Labrys clade as sister to L. chinensis. In addition to the new species description, Coslenchus rafiqi is reported from China for the first time, the description being supplemented by the first molecular data (18S and 28S rRNA) for this species.

Phylogeny of hymenolepidids (Cestoda: Cyclophyllidea) from mammals: sequences of 18S rRNA and COI genes confirm major clades revealed by the 28S rRNA analyses

2021 ◽  
Vol 95 ◽  
Author(s):  
B. Neov ◽  
G.P. Vasileva ◽  
G. Radoslavov ◽  
P. Hristov ◽  
D.T.J. Littlewood ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
18S Rrna ◽  
Phylogenetic Analyses ◽  
The Other ◽  
Rrna Genes ◽  
Host Switching ◽  
28S Rrna ◽  
Mitochondrial Cytochrome ◽  
Rapid Radiation ◽  
18S Rrna Genes

Abstract The aim of the study is to test a hypothesis for the phylogenetic relationships among mammalian hymenolepidid tapeworms, based on partial (D1–D3) nuclear 28S ribosomal RNA (rRNA) genes, by estimating new molecular phylogenies for the group based on partial mitochondrial cytochrome c oxidase I (COI) and nuclear 18S rRNA genes, as well as a combined analysis using all three genes. New sequences of COI and 18S rRNA genes were obtained for Coronacanthus integrus, C. magnihamatus, C. omissus, C. vassilevi, Ditestolepis diaphana, Lineolepis scutigera, Spasskylepis ovaluteri, Staphylocystis tiara, S. furcata, S. uncinata, Vaucherilepis trichophorus and Neoskrjabinolepis sp. The phylogenetic analyses confirmed the major clades identified by Haukisalmi et al. (Zoologica Scripta 39: 631–641, 2010): Ditestolepis clade, Hymenolepis clade, Rodentolepis clade and Arostrilepis clade. While the Ditestolepis clade is associated with soricids, the structure of the other three clades suggests multiple evolutionary events of host switching between shrews and rodents. Two of the present analyses (18S rRNA and COI genes) show that the basal relationships of the four mammalian clades are branching at the same polytomy with several hymenolepidids from birds (both terrestrial and aquatic). This may indicate a rapid radiation of the group, with multiple events of colonizations of mammalian hosts by avian parasites.

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