scholarly journals Integrative taxonomy of the genus Pseudostegana (Diptera, Drosophilidae) from China, with descriptions of eleven new species

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5160 ◽  
Author(s):  
Jinming Lu ◽  
Yuan Zhang ◽  
Hongwei Chen
Keyword(s):  
New Species ◽  
Species Delimitation ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Southwestern China ◽  
28S Rrna ◽  
Nuclear Locus ◽  
Barcode Gap

The genus Pseudostegana (Okada, 1978) currently contains thirty-nine described species. A number of Pseudostegana were collected from the fieldwork in southwestern China from 2010 to 2017. Eleven new species were discovered and are described from southwestern China: Pseudostegana alpina Zhang & Chen, sp. nov.; Pseudostegana amnicola Zhang & Chen, sp. nov.; Pseudostegana amoena Zhang & Chen, sp. nov.; Pseudostegana mailangang Zhang & Chen, sp. nov.; Pseudostegana meiduo Zhang & Chen, sp. nov.; Pseudostegana meiji Zhang & Chen, sp. nov.; Pseudostegana mystica Zhang & Chen, sp. nov.; Pseudostegana stictiptrata Zhang & Chen, sp. nov.; Pseudostegana stigmatptera Zhang & Chen, sp. nov.; Pseudostegana ximalaya Zhang & Chen, sp. nov. and Pseudostegana zhuoma Zhang & Chen, sp. nov. A key to all Chinese Pseudostegana species based on morphological characters is provided. Two mitochondrial loci (COI and ND2) and one nuclear locus (28S rRNA) were sequenced for the Pseudostegana specimens, and Bayesian and RAxML concatenated analyses were run. Molecular species delimitation is performed using the distance-based automatic barcode gap discovery (ABGD) method. Molecular data support the morphological characteristics observed among these Chinese species and confirm the new species as being distinctly different.

scholarly journals Under an integrative taxonomic approach: the description of a new species of the genus Loxosceles (Araneae, Sicariidae) from Mexico City

ZooKeys ◽  
2019 ◽  
Vol 892 ◽  
pp. 93-133 ◽  
Author(s):  
Alejandro Valdez-Mondragón ◽  
Claudia I. Navarro-Rodríguez ◽  
Karen P. Solís-Catalán ◽  
Mayra R. Cortez-Roldán ◽  
Alma R. Juárez-Sánchez
Keyword(s):  
New Species ◽  
Mexico City ◽  
Molecular Species ◽  
Species Delimitation ◽  
Morphological Characters ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Taxonomic Approach ◽  
Unique Species ◽  
A New Species

A new species of the spider genus Loxosceles Heineken & Lowe, 1832, Loxosceles tenochtitlan Valdez-Mondragón & Navarro-Rodríguez, sp. nov., is described based on adult male and female specimens from the states of Mexico City, Estado de Mexico and Tlaxcala. Integrative taxonomy including traditional morphology, geometric and lineal morphology, and molecules (DNA barcodes of cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2)), were used as evidence to delimit the new species. Four methods were used for molecular analyses and species delimitation: 1) corrected p-distances under neighbor joining (NJ), 2) automatic barcode gap discovery (ABGD), 3) general mixed yule coalescent model (GMYC), and 4) poisson tree processes (bPTP). All molecular methods, traditional, geometric and lineal morphology were consistent in delimiting and recognizing the new species. Loxosceles tenochtitlansp. nov. is closely related to L. misteca based on molecular data. Although both species are morphologically similar, the average p-distance from CO1 data was 13.8% and 4.2% for ITS2 data. The molecular species delimitation methods recovered well-supported monophyletic clusters for samples of L. tenochtitlansp. nov. from Mexico City + Tlaxcala and for samples of L. misteca from Guerrero. Loxosceles tenochtitlansp. nov. is considered a unique species for three reasons: (1) it can be distinguished by morphological characters (genitalic and somatic); (2) the four different molecular species delimitation methods were congruent to separate both species; and (3) there is variation in leg I length of males between both species, with the males of L. misteca having longer legs than males of L. tenochtitlansp. nov., also morphometrically, the shape of tibiae of the palp between males of both species is different.

Description of two new species of Rhamphus related to R. oxyacanthae (Curculionidae, Curculioninae, Rhamphini) from Italy based on a morphological study supported by molecular data

Zootaxa ◽  
2021 ◽  
Vol 4995 (1) ◽  
pp. 111-128
Author(s):  
LUCIANO DIOTTI ◽  
ROBERTO CALDARA ◽  
IVO TOŠEVSKI
Keyword(s):  
New Species ◽  
Morphological Study ◽  
Host Plants ◽  
Molecular Study ◽  
Morphological Characters ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
The Other ◽  
Distribution Data ◽  
Two New Species

Two new species of the weevil genus Rhamphus from Italy are herein described: R. bavierai n. sp. (Sicily) and R. hampsicora n. sp. (Sardinia). Both are morphologically and from a molecular perspective close to R. oxyacanthae (Marsham, 1802) and R. monzinii Pesarini & Diotti, 2012. Aside from a diagnostic description and a synoptic key, distribution data and notes on the host plants of the four species are reported. Whereas R. monzinii can be distinguished by several morphological characters, the other three species are morphologically very similar to each other and separable only by the combination of a few subtle characters. On the contrary, a preliminary molecular study revealed substantial divergences of mtCOI from 6.2 to 14.9% between the species, confirming the importance of an integrative taxonomy.  

scholarly journals Integrative taxonomy reveals two new cryptic species of Hyphessobrycon Durbin, 1908 (Teleostei: Characidae) from the Maracaçumé and middle Tocantins River basins, Eastern Amazon region

2020 ◽  
Vol 723 ◽  
pp. 77-107
Author(s):  
Erick Cristofore Guimarães ◽  
Pâmella Silva De Brito ◽  
Pedro Henrique Negreiros Bragança ◽  
Jadson Pinheiro Santos ◽  
Axel Makay Katz ◽  
...  
Keyword(s):  
New Species ◽  
Mitochondrial Gene ◽  
Morphological Characters ◽  
Molecular Data ◽  
Color Pattern ◽  
Integrative Taxonomy ◽  
The Body ◽  
Dorsal Fin ◽  
Two New Species ◽  
Molecular Phylogenetic

Two new species, Hyphessobrycon frickei Guimarães, Brito, Bragança, Katz & Ottoni sp. nov. and H. geryi Guimarães, Brito, Bragança, Katz & Ottoni sp. nov., are herein described, based on seven different and independent species delimitation methods, and on molecular and morphological characters, making the hypothesis of these new species supported from an integrative taxonomy perspective. They belong to the “Rosy tetra” clade, which is mainly characterized by the presence of a dark brown or black blotch on the dorsal fin and the absence of a midlateral stripe on the body. These two new species are distinguished from the other members of this clade mainly by the arrangement, shape and color pattern of humeral and dorsal-fin spots, as well as by other characters related to scale counts and body pigmentation. The placement of the new species within the “Rosy tetra” clade was based on the combination of morphological character states mentioned above and corroborated by a molecular phylogenetic analysis using the mitochondrial gene cytochrome oxidase subunit 1. In addition, a new clade (here termed Hyphessobrycon copelandi clade) within the “Rosy tetra” clade is proposed based on molecular data, comprising H. copelandi, H. frickei sp. nov., H. geryi sp. nov. and a still undescribed species. Our results corroborate the occurrence of hidden species within the “Rosy tetra” clade, as suggested by previous studies.

Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the “Crangonyx floridanus complex”

Zootaxa ◽  
2019 ◽  
Vol 4691 (4) ◽  
pp. 301-332 ◽  
Author(s):  
ANDREW G. CANNIZZARO ◽  
THOMAS R. SAWICKI
Keyword(s):  
New Species ◽  
Related Species ◽  
Species Delimitation ◽  
Species Complex ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Closely Related Species ◽  
Leon County ◽  
Two New Species ◽  
Morphological And Molecular Data

Crangonyx ephemerus n. sp. and Crangonyx pseudoephemerus n. sp. are described from the headwaters of the St. Marks River in Leon County, Florida, based on detailed morphological and molecular comparisons with the closely related species Crangonyx floridanus Bousfield, 1963. The morphological and molecular data, including three species delimitation models, lend support to the hypothesis that the taxon C. floridanus sensu lato represents a species complex. Diagnostic morphological characteristics are highlighted and discussed within this group to assist in future morphological analyses. 

Guidelines for DNA barcoding of coralline algae, focusing on Lithophylloideae (Corallinales) from Brazil

2018 ◽  
Vol 61 (2) ◽  
pp. 127-140 ◽  
Author(s):  
Beatriz N. Torrano-Silva ◽  
Bruno R. Vieira ◽  
Rafael Riosmena-Rodríguez ◽  
Mariana C. Oliveira
Keyword(s):  
New Species ◽  
Species Delimitation ◽  
Morphological Traits ◽  
Dna Barcode ◽  
Molecular Data ◽  
Coralline Algae ◽  
Integrative Approach ◽  
Interspecific Divergence ◽  
Algorithmic Approaches ◽  
Barcode Gap

AbstractMultiple-marker (COI-5P, UPA,psbA andrbcL-3P) and two algorithmic approaches [automatic barcode gap discovery (ABGD) and Poisson tree process (PTP)] were used for species delimitation of Lithophylloideae in Brazil. The integrative approach was mostly congruent between markers and algorithmic methods of species delimitation, suggesting the occurrence of 24 species. Based on morphology and molecular data,Amphiroa rigida,Amphiroa vanbosseae,Lithophyllum atlanticum,Lithophyllum kaiseri,Lithophyllum margaritae,Titanoderma pustulatum,Titanoderma prototypumandPaulsilvella huveorum, which were previously reported for Brazil, are confirmed in this work. Six new species are distinguished by both molecular and morphological traits, and they are provisionally named asAmphiroasp. 1,Amphiroasp. 2,Amphiroasp. 3,Lithophyllumsp. 1,Lithophyllumsp. 2 andLithophyllumsp. 3. Another 10 species are cryptic and cannot be distinguished based on traditionally used morphological traits. These includeAmphiroasp. 4,Lithophyllumsp. 4, three species that are morphologically named underAmphiroa beauvoisii, and six that share the morphology described forAmphiroa fragilissima. All four markers used were useful for species delimitation. However, a combination of practical aspects and levels of intra- and interspecific divergence values led us to propose the use ofrbcL-3P as a standard DNA barcode marker for the Corallinales.

Morphological characters and molecular data reveal a new species of Rhodonia (Polyporales, Basidiomycota) from China

Phytotaxa ◽  
2017 ◽  
Vol 328 (2) ◽  
pp. 175
Author(s):  
YUAN YUAN ◽  
LU-LU SHEN
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Dna Sequences ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Northwest China ◽  
Morphological Characters ◽  
Molecular Data ◽  
Molecular Evidence ◽  
A New Species

A new polypore, Rhodonia tianshanensis, collected from West Tianshan Nature Reserve in Xinjiang Autonomous Region (northwest China), is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by having resupinate basidiomata with an oblique tube layer, fusoid cystidioles in the hymenium, and cylindrical basidiospores. Based on multiple loci DNA sequences including the internal transcribed spacer (ITS), the large subunit (nLSU), and the second subunit of RNA polymerase II (RPB2) regions, our phylogeny strongly supported R. tianshanensis as a new species belonging to the genus Rhodonia.

scholarly journals Description of a new species of Loxosceles Heineken & Lowe (Araneae, Sicariidae) recluse spiders from Hidalgo, Mexico, under integrative taxonomy: morphological and DNA barcoding data (CO1 + ITS2)

2020 ◽  
Author(s):  
Claudia Isabel Navarro-Rodríguez ◽  
Alejandro Valdez-Mondragón
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Species Delimitation ◽  
Sister Group ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Taxonomic Approach ◽  
Morphological Differences ◽  
Unique Species ◽  
A New Species

Based on an integrative taxonomic approach, a new species of the genus Loxosceles Heineken & Lowe, 1832, is described from the state of Hidalgo, Mexico. Loxosceles tolantongo sp. nov. is described based on DNA barcoding using cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2), and morphology. For species delimitation, four molecular methods were implemented: 1) corrected p-distances under neighbor joining (NJ); 2) automatic barcode gap discovery (ABGD); 3) general mixed yule coalescent model (GMYC) and 4) Bayesian Poisson tree processes (bPTP). The new species morphologically resembles L. jaca, another species from Hidalgo, but there are morphological differences mainly in the tibiae of the male palp, the seminal receptacles of the females and also the high genetic p-distances. CO1 was more informative than ITS2 for the genetic separation; however, both concatenated genes (CO1 + ITS2) present robust evidence for species delimitation. Loxosceles tolantongo sp. nov. is considered a unique species for four reasons: 1) it can be diagnosed and distinguished by morphological characters (of the male palps mainly, but also of the seminal receptacles of the females); 2) the genetic p-distances with CO1 were high (>10%); 3) the molecular species delimitation methods were congruent under CO1 and CO1 + ITS2; and 4) under CO1 and CO1 + ITS2, the new species is a putative sister group of L. jaca + L. tenango.

­­­Laccaria rubroalba sp. nov. (Hydnangiaceae, Agaricales) from Southwestern China

Phytotaxa ◽  
2016 ◽  
Vol 284 (1) ◽  
pp. 41 ◽  
Author(s):  
XIA LUO ◽  
LEI YE ◽  
JIE CHEN ◽  
SAMANTHA C. KARUNARATHNA ◽  
JIANCHU XU ◽  
...  
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Novel Species ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Southwestern China ◽  
Its Sequence ◽  
The Novel ◽  
Closely Related Species ◽  
Line Drawings

The novel species Laccaria rubroalba is described from Southwestern China by using both morphological characteristics and molecular data. It is characterized by small basidiomata; reddish white pileus when moist or young, white to pale when dry; 4-spored basidia and globose to broadly ellipsoid, hyaline, moderately echinulate basidiospores. Phylogenetic relationships inferred from ITS sequence data confirmed the separation of this new species from other closely related species in the genus Laccaria. The new species is compared with similar taxa. A description, line drawings and colour photographs of the new species, and phylogenetic tree to show the placement of the new species are provided.

scholarly journals Description of Longidorus cheni sp. n. (Nematoda, Longidoridae) from China

ZooKeys ◽  
2018 ◽  
Vol 744 ◽  
pp. 1-18 ◽  
Author(s):  
Eda Marie Barsalote ◽  
Hoa Thi Pham ◽  
Stela Lazarova ◽  
Vlada Peneva ◽  
Jingwu Zheng
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Molecular Data ◽  
Rrna Genes ◽  
28S Rrna ◽  
Oral Aperture ◽  
Asiatic Origin ◽  
18S Rrna Genes ◽  
Guide Ring ◽  
Partial 18S

Longidoruschenisp. n., an amphimictic species recovered from the rhizosphere ofLarixprincipis-rupprechtiiandPyracanthafortuneanain Shanxi and Beijing, China, is described and illustrated. The taxonomic position ofL.chenisp. n.among other species within the genus was elucidated using morphometric and molecular data, and phylogenetic relationships were inferred using D2–D3 expansion domains of 28S and 18S rRNA genes by Bayesian Inference (BI) method. The new species is characterised by females with a medium body size (L = 4.9–6.6 mm), a lip region slightly expanded, broadly rounded frontally and laterally, the amphidial fovea broad and symmetrically bilobed at base, odontostyle long and slender (143–168 μm), odonthophore slightly swollen at the base, tail short bluntly conoid to rounded. Guide ring located far posterior from the oral aperture (70–93 μm). Males with two ad-cloacal pairs of supplements preceded by a row of 10–14 ventromedian supplements, with robust spicules measuring 111–126 μm along the median line. Three juvenile stages were present, tail shape of J1 elongate conoid while in J2 and J3 the tail gradually becomes bluntly rounded. Codes for identifying the new species are: A6-B3-C5-D2-E2-F3-G1-H1-I2-J2-K2.Longidoruschenisp. n.belongs to a group of species with a guide ring at the mid-odontostyle position that have a predominantly Asiatic origin. It differs from all of them by a combination of morphological characters and unique sequences of partial 18S and D2–D3 region of 28S rRNA genes. The percentage dissimilarities in partial 18S and D2–D3 28S rRNA genes ofL.chenito the closest species (L.litchii,L.fangi,L.jonesiandL.juglans) were 1.5 %–1.8 % and 16.8–18.3 %, respectively.

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