scholarly journals Characterization of proteinase inhibitor-II gene under OsRGLP2promoter for salt stress in transgenic Nicotiana benthamiana

2017 ◽  
Vol 41 ◽  
pp. 494-502 ◽  
Author(s):  
Shazia REHMAN ◽  
Bodil JØRGENSEN ◽  
Søren Kjærsgaard RASMUSSEN ◽  
Tariq MAHMOOD
Keyword(s):  
Salt Stress ◽  
Nicotiana Benthamiana ◽  
Proteinase Inhibitor ◽  
Proteinase Inhibitor Ii

scholarly journals Proteinase inhibitors in rat serum. Purification and partial characterization of three functionally distinct trypsin inhibitors

1984 ◽  
Vol 218 (3) ◽  
pp. 953-959 ◽  
Author(s):  
L Kuehn ◽  
M Rutschmann ◽  
B Dahlmann ◽  
H Reinauer
Keyword(s):  
Proteinase Inhibitor ◽  
Proteinase Inhibitors ◽  
Serine Proteinase ◽  
The Other ◽  
Serine Proteinases ◽  
Rat Serum ◽  
Apparent Homogeneity ◽  
Human Counterpart ◽  
Proteinase Inhibitor Ii

Three different serine proteinase inhibitors were isolated from rat serum and purified to apparent homogeneity. One of the inhibitors appears to be homologous to alpha 1-proteinase inhibitor isolated from man and other species, but the other two, designated rat proteinase inhibitor I and rat proteinase inhibitor II, seem to have no human counterpart. alpha 1-Proteinase inhibitor (Mr 55000) inhibits trypsin, chymotrypsin and elastase, the three serine proteinases tested. Rat proteinase inhibitor I (Mr 66000) is active towards trypsin and chymotrypsin, but is inactive towards elastase. Rat proteinase inhibitor II (Mr 65000) is an effective inhibitor of trypsin only. Their contributions to the trypsin-inhibitory capacity of rat serum are about 68, 14 and 18% for alpha 1-proteinase inhibitor, rat proteinase inhibitor I and rat proteinase inhibitor II respectively.

Proteinase inhibitor II from potatoes: isolation and characterization of its protomer components

Biochemistry ◽  
1976 ◽  
Vol 15 (16) ◽  
pp. 3418-3424 ◽  
Author(s):  
Joel Bryant ◽  
Terrence R. Green ◽  
T. Gurusaddaiah ◽  
Clarence A. Ryan
Keyword(s):  
Proteinase Inhibitor ◽  
Isolation And Characterization ◽  
Proteinase Inhibitor Ii

Characterization of potato proteinase inhibitor II reactive site mutants

2000 ◽  
Vol 267 (7) ◽  
pp. 1975-1984 ◽  
Author(s):  
Jules Beekwilder ◽  
Bert Schipper ◽  
Petra Bakker ◽  
Dirk Bosch ◽  
Maarten Jongsma
Keyword(s):  
Proteinase Inhibitor ◽  
Reactive Site ◽  
Proteinase Inhibitor Ii ◽  
Potato Proteinase Inhibitor Ii

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

scholarly journals Genome-Wide Identification and Characterization of Long Noncoding RNAs Involved in Chinese Wheat Mosaic Virus Infection of Nicotiana benthamiana

Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 232
Author(s):  
Weiran Zheng ◽  
Haichao Hu ◽  
Qisen Lu ◽  
Peng Jin ◽  
Linna Cai ◽  
...  
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Differentially Expressed ◽  
Genome Wide ◽  
Chinese Wheat

Recent studies have shown that a large number of long noncoding RNAs (lncRNAs) can regulate various biological processes in animals and plants. Although lncRNAs have been identified in many plants, they have not been reported in the model plant Nicotiana benthamiana. Particularly, the role of lncRNAs in plant virus infection remains unknown. In this study, we identified lncRNAs in N. benthamiana response to Chinese wheat mosaic virus (CWMV) infection by RNA sequencing. A total of 1175 lncRNAs, including 65 differentially expressed lncRNAs, were identified during CWMV infection. We then analyzed the functions of some of these differentially expressed lncRNAs. Interestingly, one differentially expressed lncRNA, XLOC_006393, was found to participate in CWMV infection as a precursor to microRNAs in N. benthamiana. These results suggest that lncRNAs play an important role in the regulatory network of N. benthamiana in response to CWMV infection.

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