Recombinant outer membrane protein Q and putative lipoprotein from Bordetella pertussis inducing strong humoral response were not protective alone in the murine lung colonization model

Immunization with the 69-kD outer membrane protein (OMP) of Bordetella pertussis protected neonatal mice against lethal respiratory challenge with B. pertussis 18323. Active immunization elicited a serum IgG anti-69-kD OMP response at the time of challenge, with IgG anti-69-kD OMP antibodies detected in bronchoalveolar lavage fluid after challenge. Intravenous administration of BPE8, a monoclonal IgG1 anti-69-kD OMP, also protected young mice against B. pertussis challenge. Intravenously injected BPE8 was detected in the lungs of mice at the time of aerosol challenge, suggesting that the presence of specific antibody in the lungs may mediate protection. Thus the 69-kD OMP of B. pertussis is a protective antigen in mice that elicits specific serum antibody that can transude to the lung. The 69-kD OMP was detected in a preparation of a Takeda acellular vaccine by immunoblot analysis and a serum antibody response to the 69-kD OMP was observed in 18-mo-old children boosted with this preparation of Japanese acellular vaccine. Our results demonstrate that the B. pertussis 69-kD OMP is a protective antigen in animals, is immunogenic in humans, and is present in a preparation of acellular pertussis vaccine that is widely used in Japan. These findings indicate that the 69-kD OMP should be seriously considered as a candidate for inclusion in new formulations of antigenically defined acellular pertussis vaccines.

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Polymorphism of Repeated Regions of Pertactin in Bordetella pertussis, Bordetella parapertussis, andBordetella bronchiseptica

Infection and Immunity ◽

10.1128/iai.68.8.4815-4817.2000 ◽

2000 ◽

Vol 68 (8) ◽

pp. 4815-4817 ◽

Cited By ~ 28

Author(s):

Caroline Boursaux-Eude ◽

Nicole Guiso

Keyword(s):

Membrane Protein ◽

Outer Membrane ◽

Bordetella Pertussis ◽

Outer Membrane Protein ◽

Protective Immunity ◽

Bordetella Bronchiseptica ◽

Bordetella Parapertussis ◽

Region I ◽

Region Ii

ABSTRACT Pertactin is an outer membrane protein expressed byBordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica that induces protective immunity to Bordetella infections. The immunodominant and immunoprotective epitopes of pertactin include two repeated regions, I and II. Comparison of these two repeated regions showed that B. parapertussis pertactin is invariant, whereas B. pertussis pertactin varies mostly in region I and B. bronchiseptica pertactin varies in both repeated regions I and II, but mostly in region II. These differences may result from specific characteristics of these Bordetella species.

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Characterization of the humoral response induced by a synthetic peptide of the major outer membrane protein of Chlamydia trachomatis serovar B.

Infection and Immunity ◽

10.1128/iai.62.8.3547-3549.1994 ◽

1994 ◽

Vol 62 (8) ◽

pp. 3547-3549 ◽

Cited By ~ 2

Author(s):

A Villeneuve ◽

L Brossay ◽

G Paradis ◽

J Hébert

Keyword(s):

Chlamydia Trachomatis ◽

Membrane Protein ◽

Outer Membrane ◽

Outer Membrane Protein ◽

Synthetic Peptide ◽

Humoral Response ◽

Major Outer Membrane Protein

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Construction and characterization of Bordetella pertussis mutants lacking the vir-regulated P.69 outer membrane protein

Molecular Microbiology ◽

10.1111/j.1365-2958.1991.tb00786.x ◽

1991 ◽

Vol 5 (6) ◽

pp. 1393-1404 ◽

Cited By ~ 55

Author(s):

M. Roberts ◽

N. F. Fairweather ◽

E. Leininger ◽

D. Pickard ◽

E. L. Hewlett ◽

...

Keyword(s):

Membrane Protein ◽

Outer Membrane ◽

Bordetella Pertussis ◽

Outer Membrane Protein

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Biochemical and immunological properties of two forms of pertactin, the 69,000-molecular-weight outer membrane protein of Bordetella pertussis.

Infection and Immunity ◽

10.1128/iai.61.5.2211-2215.1993 ◽

1993 ◽

Vol 61 (5) ◽

pp. 2211-2215 ◽

Cited By ~ 16

Author(s):

J W Gotto ◽

T Eckhardt ◽

P A Reilly ◽

J V Scott ◽

J L Cowell ◽

...

Keyword(s):

Molecular Weight ◽

Membrane Protein ◽

Outer Membrane ◽

Bordetella Pertussis ◽

Outer Membrane Protein ◽

Immunological Properties

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Recombinant PorA, the Major Outer Membrane Protein of Campylobacter jejuni, Provides Heterologous Protection in an Adult Mouse Intestinal Colonization Model

Clinical and Vaccine Immunology ◽

10.1128/cvi.00255-10 ◽

2010 ◽

Vol 17 (11) ◽

pp. 1666-1671 ◽

Cited By ~ 20

Author(s):

Anjum Islam ◽

Raj Raghupathy ◽

M. John Albert

Keyword(s):

Membrane Protein ◽

Outer Membrane ◽

Campylobacter Jejuni ◽

Outer Membrane Protein ◽

Lavage Fluid ◽

Major Outer Membrane Protein ◽

Enzyme Linked Immunosorbent Assay ◽

Food Borne ◽

Colonization Model ◽

O 19

ABSTRACT Immunity against Campylobacter jejuni, a major food-borne pathogen causing diarrhea, is largely serotype specific. The major outer membrane protein (MOMP) of C. jejuni, PorA, is a common antigen with the potential to provide broad protection. Adult BALB/c mice were orally immunized with a recombinant glutathione S-transferase (GST) fused to PorA prepared from Campylobacter jejuni C31 (O:6,7) (GST-PorA) combined with a modified heat-labile enterotoxin of Escherichia coli as an adjuvant and later orally challenged with C31 strain or three heterologous strains: 48 (O:19), 75 (O:3), and 111 (O:1,44). Protection from colonization with the challenge organism was studied by fecal screening daily for 9 days. Serum and intestinal lavage fluid antibodies against the vaccine and Sarkosyl-purified MOMP from C31 were measured by using an enzyme-linked immunosorbent assay. The vaccine produced robust antibody responses against both antigens in serum and secretion. Since strain C31 was a poor colonizer, homologous protection could not be studied. The protective efficacies of heterologous strains were 43% (for strain 48, P < 0.001), 29% (for strain 75, P < 0.005), and 42% (for strain 111, P < 0.001) for the 9-day period compared to control mice given phosphate-buffered saline. Thus, PorA provided appreciable protection against colonization with heterologous serotypes.

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