The combined effect of two rapid multiplication techniques (sprout and top-shoot cutting) was
assessed using four Dutch potato cultivars during 1991/92–1993/1994 at the Tuber Crops Research
Centre (TCRC), Bangladesh Agricultural Research Institute (BARI), Gazipur-1701, Bangladesh. On
average, a 70–80 g tuber produced 29 sprout cuttings which were subsequently used for taking top-shoot cuttings. Top-shoot cuttings were taken at a number of dates from 25 November and continued
at 10 day intervals, thereafter, until 15 January. The number of top-shoot cuttings produced by 29
sprout cutting-derived plants varied (P<0·01) from 761 to 663 across the cultivars. Cardinal gave
the greatest yield. Most of the characters studied varied (P<0·01) between cultivars but not over
years while the interaction between cultivars and years was significant (P<0·01).Early planted top-shoot cuttings produced more tubers per plant (4·0) with a greater mean tuber
weight (>22 g) than from the late planting (1 tuber and 5·5 g). On average, about 2·8 tubers per plant
were produced with a mean tuber weight of 15·8 g. Cardinal and Patrones produced the most tubers
per plant (2·9) and Diamant the greatest mean tuber weight (16·8 g). Cardinal gave the greatest yield
(P<0·01) of tubers (268) followed by Patrones (241). The 29 sprout cutting plants derived from a
70±5 g tuber produced 160 tubers while the 771 top-shoot plants derived from 29 sprout cutting-derived plants produced 1420 tubers. The rate of multiplication of the combined two RM techniques
was 1580[ratio ]1 against 11[ratio ]1 for the conventional method.
Individual and combined effect of drought and heat stresses in contrasting potato cultivars overexpressing miR172b-3p
