scholarly journals Comparison of DNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP

2010 ◽  
Vol 9 (11) ◽  
pp. 1660-1663
Author(s):  
Reza Nahavandi ◽  
Nader Asadzadeh ◽  
Adbdolreza Soleimani ◽  
S.M. Nurul Amin ◽  
Parivash Hafezamini ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Polymorphism ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Specific Transcription Factor ◽  
Pcr Rflp

scholarly journals Genetic Variation of Calpastation Gene of Indigenous Bali Cattle (Bos javanicus) in Indonesia

2019 ◽  
Vol 26 (1) ◽  
pp. 44
Author(s):  
Endang Tri Margawati ◽  
Slamet Diah Volkandari ◽  
Indriawati Indriawati ◽  
Emma M. Svensson
Keyword(s):  
Genetic Variation ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Meat Tenderness ◽  
Gene Markers ◽  
Bos Javanicus ◽  
Restriction Fragment Polymorphism ◽  
Pcr Rflp ◽  
Bali Cattle ◽  
Cast Gene

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.

scholarly journals DNA Polymorphism in SLC11A1 Gene and its Association with Brucellosis Resistance in Indian Zebu (Bos indicus) and Crossbred (Bos indicus×Bos taurus) Cattle

2011 ◽  
Vol 24 (7) ◽  
pp. 898-904 ◽  
Author(s):  
Nishant Kumar ◽  
Indrajit Ganguly ◽  
Rajendra Singh ◽  
Sitangsu M. Deb ◽  
Subodh Kumar ◽  
...  
Keyword(s):  
Dna Polymorphism ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Slc11a1 Gene

DNA Polymorphism of BoLA-DRB3.2 Gene in Tharparkar Cattle (Bos indicus) by PCR-RFLP

2007 ◽  
Vol 32 (2) ◽  
pp. 187-190
Author(s):  
Bharat Bhushan ◽  
B. N. Patra ◽  
Chandan Paswan ◽  
Umang ◽  
Pushpendra Kumar ◽  
...  
Keyword(s):  
Dna Polymorphism ◽  
Bos Indicus ◽  
Tharparkar Cattle ◽  
Pcr Rflp

scholarly journals The polymorphism in g.1256G>A of bovine pituitary specific transcription factor-1 (bPIT-1) gene and its association with body weight of Pasundan cattle

2019 ◽  
Vol 44 (1) ◽  
pp. 19 ◽  
Author(s):  
W. P. B. Putra ◽  
P. P. Agung ◽  
S. Said
Keyword(s):  
Transcription Factor ◽  
Body Weight ◽  
Pituitary Gland ◽  
Chi Square ◽  
Specific Transcription Factor ◽  
Breeding Station ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
Informative Content ◽  
Transcription Factor 1

Bovine Pituitary specific transcription factor 1 (bPit-1) is one of amino acid that controling pituitary gland in mammals. The pituitary gland is important for secretion of growth hormone from growth genes. This study was carried out to detect polymorphism in the exon 6 of bPit-1 (g.1256G>A) in Pasundan cattle using PCR-RFLP method and its association with body weight. Total of 69 heads (15 males and 54 females) of Pasundan cattle from breeding station (BPPIBT-SP Ciamis, West Java) were used in this study. Research showed that two genotypes of bPit-1/HinfI gene were identified in this study i.e GG (0.90) and AG (0.10) with allele frequencies of 0.05 (A) and 0.95 (G). The polymorphic informative content (PIC) and number of effective allele (ne) values were 0.09 (low) and 1.11. respectively. The Chi-square (χ2) value in the population studied was 0.20 and in Hardy-Weinberg equilibrium (χ2<5.99). It was concluded that the polymorphism of bPit-1/HinfI in Pasundan cattle included of low category and was not associated with body weight.

scholarly journals Genotyping of the polymorphism within exon 3 of prolactin gene in various dairy breeds by PCR RFLP (Brief report)

2008 ◽  
Vol 51 (3) ◽  
pp. 298-299 ◽  
Author(s):  
A. Ratna Kumari ◽  
K. M. Singh ◽  
K. J. Soni ◽  
R. K. Patel ◽  
J. B. Chauhan ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Dairy Cattle ◽  
Genetic Characterization ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Genotype Frequencies ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Transition Mutation

Abstract. In mammals, especially dairy cattle the prolactin has important functions like the development of mammary gland affecting milk yield and composition. It has been mapped to chromosome 23 in Bovine (HALLERMAN et al., 1988). A silent A→G transition mutation at the codon for amino acid 103 in exon 3 of bovine prolactin (bPRL) gene gives rise to a polymorphic Rsa I site, has become a popular genetic marker used for genetic characterization of cattle populations by means of PCR-RFLP (MITRA et al., 1995; CHRENEK et al., 1998; DYBUS, 2005). The present study reports on the genotype frequencies observed in various Bos taurus and Bos indicus dairy cattle breeds.

scholarly journals Semen Characteristics and Genotyping of Pituitary-Specific Transcription Factor Gene in Buffalo Using PCR-RFLP

2016 ◽  
Vol 47 (1) ◽  
pp. 13-26
Author(s):  
M.H. Hasanain*, ◽  
Karima Mahmoud*, ◽  
A.A. El-Menoufy**, ◽  
A.M. Sakr ***, ◽  
Y.F. Ahmed* ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Specific Transcription Factor ◽  
Pcr Rflp ◽  
Semen Characteristics

Sequence characterization and SNP identification of TNP1 gene in Indian cattle breeds

2017 ◽  
Author(s):  
Ashish Ranjan ◽  
K. Raja ◽  
Ranjana Sinha ◽  
I. Ganguly ◽  
I. Gupta ◽  
...  
Keyword(s):  
Promoter Region ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Coding Region ◽  
Sequence Characterization ◽  
Holstein Breed ◽  
Cattle Breeds ◽  
Pcr Rflp ◽  
Pcr Products ◽  
Indian Cattle

Present study was conducted on 50 bulls and 40 male calves of Sahiwal, Tharparkar and Karan Fries cattle maintained at ABRC and LRC, NDRI Karnal (Haryana) to characterize and identify genetic polymorphisms in TNP1 gene. A total of 1568 bp region of TNP-1 gene includes 490 bp of promoter region and two exon and one intron was sequenced and characterized in Bos indicus cattle breeds which are widely distributed in Indian sub-continent. Four sets of primers for TNP1 gene on the basis of Bos Taurus sequence (Acc. No- BK_006511) were designed using Primer3 software and PCR products of 487, 450, 455 and 250 bp were obtained. Amplicons were custom sequenced and subjected to Clustal W analysis which showed no nucleotide changes in coding region and non coding region in Indian cattle breeds as compared to Bos taurus. The 490 bp of promoter region was subjected to transcription factor binding site. Three TATA boxes and two CAAT boxes were identified in the studied fragment. Analysis of SNP was performed using restriction fragment length polymorphism (PCR-RFLP), to detect nucleotide changes in the sequence as reported (g.528G>A, SS1388116558) in Chinese Holstein breed. No polymorphisms were found for tested SNP. Only one genotype GG indicates the absence of variability in the sampled population.

scholarly journals Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene

2008 ◽  
Vol 43 (5) ◽  
pp. 657-659 ◽  
Author(s):  
Rogério Abdallah Curi ◽  
Luis Artur Loyola Chardulo ◽  
Antônio Carlos Silveira ◽  
Henrique Nunes de Oliveira
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Bos Taurus ◽  
Direct Sequencing ◽  
Bos Indicus ◽  
Basic Structure ◽  
Meat Tenderness ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Pcr Rflp ◽  
Cast Gene

The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR-RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR-RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facilitate its analysis by laboratories with basic structure.

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