DNA Polymorphism of BoLA-DRB3.2 Gene in Tharparkar Cattle (Bos indicus) by PCR-RFLP

Present study molecular characterization of exon 2 of TLR2 gene and its association with milk yield and milk quality traits in 104 Deoni cattle using PCR- RFLP technique was done. Polymorphism was observed through HaeIII, HhaI and EcoRV restriction enzymes in Created Restriction Site (CRS) exon 2-1, CRS exon 2-5 and exon 2-1 by PCR- RFLP, respectively. In CRS exon 2-1 allelic frequencies were observed as 0.793 for A and 0.206 for B alleles and that of genotypic frequencies were 0.58 and 0.41 for genotypes AA and AB. In CRS exon 2-5, two genotypes viz., AC and CC with corresponding allelic frequencies were observed as 0.221 for A and 0.778 for C allele and that of genotypic frequencies observed were 0.44 and 0.55 for AC and CC genotypes respectively. TLR2 exon 2-1 exhibited two alleles G and T with frequencies of 0.134 and 0.865 and their Corresponding genotypic frequencies were 0.009, 0.25 and 0.74for GG, GT and TT genotypes respectively. Higher count of somatic cells (SCC) in TT homozygous and TG heterozygous genotypes, and lower in GG homozygous genotypes were observed in exon 2-1. Strongly significant (P£0.01) effect for least squares means of Test Day milk yield (TDMY) and Somatic Cell Count of CRS exon 2-1 were observed.

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Genetic Variation of Calpastation Gene of Indigenous Bali Cattle (Bos javanicus) in Indonesia

HAYATI Journal of Biosciences ◽

10.4308/hjb.26.1.44 ◽

2019 ◽

Vol 26 (1) ◽

pp. 44

Author(s):

Endang Tri Margawati ◽

Slamet Diah Volkandari ◽

Indriawati Indriawati ◽

Emma M. Svensson

Keyword(s):

Genetic Variation ◽

Bos Taurus ◽

Bos Indicus ◽

Meat Tenderness ◽

Gene Markers ◽

Bos Javanicus ◽

Restriction Fragment Polymorphism ◽

Pcr Rflp ◽

Bali Cattle ◽

Cast Gene

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.

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Genetic Variants of β-casein Gene in Indigenous and Exotic Dairy Cattle Breeds in Sri Lanka

Asian Journal of Dairy and Food Research ◽

10.18805/ajdfr.dr-180 ◽

2020 ◽

Author(s):

S. Wickramasinghe ◽

R. K. Rupasinghe ◽

N. Shanjayan ◽

L. G.S. Lokugalappatti

Keyword(s):

Sri Lanka ◽

Bos Indicus ◽

Bovine Milk ◽

Cattle Population ◽

Health Issues ◽

Genotype Frequencies ◽

Holstein Friesian ◽

Pcr Rflp ◽

Hardy Weinberg Equilibrium ◽

White Cattle

Background: Bovine milk contains two types of β-casein (CSN2); A1 and A2, in which A1 type has shown associations with certain health issues. This study was conducted to identify the abundance of CSN2 A1/A2 variants in European and indigenous dairy breeds in Sri Lanka. Methods: The study included 123 Ayrshire, 101 Holstein Friesian and 92 indigenous White cattle (Bos indicus) populations. Genotyping was performed by PCR-RFLP method using Dde1. Allele and genotype frequencies and population genetic indices were calculated. Result: Results showed A1A2 to be predominant in both Holstein Friesian (0.63) and Ayrshire (0.68) populations while A2A2 (0.82) was predominant in the White cattle population. A1 and A2 allele frequencies were 0.52 and 0.48 in Ayrshire; 0.39 and 0.61 in Holstein Friesian and 0.09 and 0.91 in White cattle populations. Ayrshire and Holstein Friesian populations showed significant deviation from the Hardy-Weinberg equilibrium (p£0.05). When compared to the imported breeds, the White cattle population showed inbreeding and less genetic diversity.

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DNA polymorphism in morels: PCR/RFLP analysis of the ribosomal DNA spacers and microsatellite-primed PCR

Mycological Research ◽

10.1016/s0953-7562(96)80101-8 ◽

1996 ◽

Vol 100 (1) ◽

pp. 63-71 ◽

Cited By ~ 49

Author(s):

François Buscot ◽

Daniel Wipf ◽

Céline Di Battista ◽

Jean-Charles Munch ◽

Bernard Botton ◽

...

Keyword(s):

Ribosomal Dna ◽

Dna Polymorphism ◽

Rflp Analysis ◽

Pcr Rflp

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DNA Polymorphism in SLC11A1 Gene and its Association with Brucellosis Resistance in Indian Zebu (Bos indicus) and Crossbred (Bos indicus×Bos taurus) Cattle

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.2011.10306 ◽

2011 ◽

Vol 24 (7) ◽

pp. 898-904 ◽

Cited By ~ 4

Author(s):

Nishant Kumar ◽

Indrajit Ganguly ◽

Rajendra Singh ◽

Sitangsu M. Deb ◽

Subodh Kumar ◽

...

Keyword(s):

Dna Polymorphism ◽

Bos Taurus ◽

Bos Indicus ◽

Slc11a1 Gene

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Genotyping of the polymorphism within exon 3 of prolactin gene in various dairy breeds by PCR RFLP (Brief report)

Archives Animal Breeding ◽

10.5194/aab-51-298-2008 ◽

2008 ◽

Vol 51 (3) ◽

pp. 298-299 ◽

Cited By ~ 2

Author(s):

A. Ratna Kumari ◽

K. M. Singh ◽

K. J. Soni ◽

R. K. Patel ◽

J. B. Chauhan ◽

...

Keyword(s):

Mammary Gland ◽

Dairy Cattle ◽

Genetic Characterization ◽

Bos Taurus ◽

Bos Indicus ◽

Genotype Frequencies ◽

Prolactin Gene ◽

Pcr Rflp ◽

Transition Mutation

Abstract. In mammals, especially dairy cattle the prolactin has important functions like the development of mammary gland affecting milk yield and composition. It has been mapped to chromosome 23 in Bovine (HALLERMAN et al., 1988). A silent A→G transition mutation at the codon for amino acid 103 in exon 3 of bovine prolactin (bPRL) gene gives rise to a polymorphic Rsa I site, has become a popular genetic marker used for genetic characterization of cattle populations by means of PCR-RFLP (MITRA et al., 1995; CHRENEK et al., 1998; DYBUS, 2005). The present study reports on the genotype frequencies observed in various Bos taurus and Bos indicus dairy cattle breeds.

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