scholarly journals Genotyping of the polymorphism within exon 3 of prolactin gene in various dairy breeds by PCR RFLP (Brief report)

2008 ◽  
Vol 51 (3) ◽  
pp. 298-299 ◽  
Author(s):  
A. Ratna Kumari ◽  
K. M. Singh ◽  
K. J. Soni ◽  
R. K. Patel ◽  
J. B. Chauhan ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Dairy Cattle ◽  
Genetic Characterization ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Genotype Frequencies ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Transition Mutation

Abstract. In mammals, especially dairy cattle the prolactin has important functions like the development of mammary gland affecting milk yield and composition. It has been mapped to chromosome 23 in Bovine (HALLERMAN et al., 1988). A silent A→G transition mutation at the codon for amino acid 103 in exon 3 of bovine prolactin (bPRL) gene gives rise to a polymorphic Rsa I site, has become a popular genetic marker used for genetic characterization of cattle populations by means of PCR-RFLP (MITRA et al., 1995; CHRENEK et al., 1998; DYBUS, 2005). The present study reports on the genotype frequencies observed in various Bos taurus and Bos indicus dairy cattle breeds.

Characterization of open reading frame-expressed sequence tags generated from Bos indicus and B. taurus mammary gland cDNA libraries

2004 ◽  
Vol 35 (3) ◽  
pp. 213-219 ◽  
Author(s):  
A. F. Mota ◽  
T. S. Sonstegard ◽  
C. P. Van Tassell ◽  
L. L. Shade ◽  
L. K. Matukumalli ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Expressed Sequence Tags ◽  
Bos Indicus ◽  
Open Reading Frame ◽  
Cdna Libraries ◽  
Reading Frame ◽  
Expressed Sequence

scholarly journals Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Widya Pintaka Bayu Putra ◽  
Saiful Anwar ◽  
Syahruddin Said ◽  
Romanos Albert Adhitya Indarto ◽  
Putri Wulandari
Keyword(s):  
Genetic Characterization ◽  
Rare Allele ◽  
Basic Information ◽  
Dominant Allele ◽  
West Java ◽  
Pcr Rflp ◽  
Molecular Selection ◽  
Lep Gene ◽  
Informative Content

The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RFLP method. Research shows that TG gene of Pasundan cattle is monomorphic with C allele as the dominant allele (1.00). However, LEP gene of Pasundan cattle is polymorphic with C allele as the dominant allele (0.98) and T as the rare allele (0.02). The polymorphic informative content (PIC) and numberof effective allele (ne) values in the LEP gene in the animal studied were 0.04 and 1.04 respectively. It was concluded that TG/BstYI and LEP/Sau3AI gene in the present study can not be used as molecular selection in Pasundan cattle. These results are important as the basic information for preparing the molecular selection program in the future.

scholarly journals Differential expression of microRNAs associated with thermal stress in Frieswal (Bos taurus x Bos indicus) crossbred dairy cattle

2017 ◽  
Vol 23 (1) ◽  
pp. 155-170 ◽  
Author(s):  
Gyanendra Singh Sengar ◽  
Rajib Deb ◽  
Umesh Singh ◽  
T. V. Raja ◽  
Rajiv Kant ◽  
...  
Keyword(s):  
Thermal Stress ◽  
Dairy Cattle ◽  
Differential Expression ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Crossbred Dairy Cattle

Association of genetic variants of the prolactin gene with milk production traits in Russian Red Pied cattle

2007 ◽  
Vol 2007 ◽  
pp. 156-156 ◽  
Author(s):  
Masoud Alipanah ◽  
Lobov Kalashnikova ◽  
Genadi Rodionov
Keyword(s):  
Genetic Marker ◽  
Regulatory Function ◽  
Cdna Clones ◽  
Production Traits ◽  
Coding Region ◽  
Nucleotide Substitutions ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Rsai Polymorphism

Prolactin plays an important regulatory function in mammary gland development, milk secretion, and expression of milk protein genes. Hence the PRL gene is a potential genetic marker of production traits in dairy cattle. The gene was mapped on chromosome 23 by Hallerman et al. (1988). It consists of 5 exons and four introns (Camper et al. 1984) encoding the 199-amino-acid mature protein (Wallis 1974). On the basis of sequence analysis of four different cDNA clones, seven possible nucleotide substitutions were described by Sasavage et al. (1982). One of them, recognized by RsaI endonuclease, has become a popular genetic marker used for genetic characterization of cattle populations by means of PCR-RFLP (Mitra et al., 1995). Two allelic variants (B and b) have been distinguished at the DNA level, based on RsaI polymorphism in the third exon of the coding region. It has been suggested that prolactin alleles correlate with milk yield (Lewin et al., 1992).

Studies on TLR2 gene variants and their association with milk yield and milk quality traits in Bos indicus (Deoni) cattle

2017 ◽  
Author(s):  
U. T. Mundhe ◽  
D. N. Das ◽  
R. S. Gandhi ◽  
P. Divya
Keyword(s):  
Milk Yield ◽  
Bos Indicus ◽  
Restriction Enzymes ◽  
Milk Quality ◽  
Quality Traits ◽  
Exon 2 ◽  
Allelic Frequencies ◽  
Tlr2 Gene ◽  
Pcr Rflp

Present study molecular characterization of exon 2 of TLR2 gene and its association with milk yield and milk quality traits in 104 Deoni cattle using PCR- RFLP technique was done. Polymorphism was observed through HaeIII, HhaI and EcoRV restriction enzymes in Created Restriction Site (CRS) exon 2-1, CRS exon 2-5 and exon 2-1 by PCR- RFLP, respectively. In CRS exon 2-1 allelic frequencies were observed as 0.793 for A and 0.206 for B alleles and that of genotypic frequencies were 0.58 and 0.41 for genotypes AA and AB. In CRS exon 2-5, two genotypes viz., AC and CC with corresponding allelic frequencies were observed as 0.221 for A and 0.778 for C allele and that of genotypic frequencies observed were 0.44 and 0.55 for AC and CC genotypes respectively. TLR2 exon 2-1 exhibited two alleles G and T with frequencies of 0.134 and 0.865 and their Corresponding genotypic frequencies were 0.009, 0.25 and 0.74for GG, GT and TT genotypes respectively. Higher count of somatic cells (SCC) in TT homozygous and TG heterozygous genotypes, and lower in GG homozygous genotypes were observed in exon 2-1. Strongly significant (P£0.01) effect for least squares means of Test Day milk yield (TDMY) and Somatic Cell Count of CRS exon 2-1 were observed.

Bos indicus and Bos taurus crossbred dairy cattle in Australia. IV.* Progeny testing and expected rate of genetic improvement

1976 ◽  
Vol 27 (2) ◽  
pp. 309 ◽  
Author(s):  
IR Franklin ◽  
RH Hayman ◽  
RO Hewetson
Keyword(s):  
Dairy Cattle ◽  
Heat Tolerance ◽  
Genetic Improvement ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Progeny Testing ◽  
Dairy Farmers ◽  
Improvement Program ◽  
Tropical Environments ◽  
Tick Resistance

A dairy improvement program designed to develop a breed of cattle adapted to tropical environments is described. Each year young crossbred (Bos indicus x Bos taurus) bulls are screened for heat tolerance and tick resistance, and then progeny-tested in the herds of cooperating dairy farmers. Estimates of phenotypic and genetic means, variances and correlations are presented for production of milk and milk components, and the rate of genetic improvement is discussed. In particular the heritability of milk yield in the crossbred progeny is 0.27, and the theoretical rate of improvement is 2.6% per year. ____________________ *Part III, Aust. J. Agric. Res., 25: 1023 (1974).

scholarly journals Genetic Variation of Calpastation Gene of Indigenous Bali Cattle (Bos javanicus) in Indonesia

2019 ◽  
Vol 26 (1) ◽  
pp. 44
Author(s):  
Endang Tri Margawati ◽  
Slamet Diah Volkandari ◽  
Indriawati Indriawati ◽  
Emma M. Svensson
Keyword(s):  
Genetic Variation ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Meat Tenderness ◽  
Gene Markers ◽  
Bos Javanicus ◽  
Restriction Fragment Polymorphism ◽  
Pcr Rflp ◽  
Bali Cattle ◽  
Cast Gene

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.

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