scholarly journals Biochemical Characterization, Activity Comparison and Isoenzyme Analysis of Amylase and Alkaline Proteases in Seven Cyprinid Fishes

2017 ◽  
Vol 12 (6) ◽  
pp. 264-272 ◽  
Author(s):  
Chamaiporn Champasri ◽  
Thongchai Champasri
Parasitology ◽  
1994 ◽  
Vol 109 (4) ◽  
pp. 497-509 ◽  
Author(s):  
J. J. McNamara ◽  
G. Mohammed ◽  
W. C. Gibson

SUMMARYWe provide evidence from isoenzyme analysis, hybridization with repetitive DNA probes, behavioural studies and morphometrics that 4 trypanosome isolates from Glossina morsitans submorsitans in The Gambia constitute a new species now named Trypanosoma (Nannomonas) godfreyi. The bloodstream trypomastigotes of T. (N.) godfreyi are relatively small with a mean length of 13·7 μm (range: 9·1–21·8 μm) and a mean width of 1·65 μm (range: 0·65–2·69 μm). There is no free flagellum and the marginal kinetoplast is subterminal to a rounded posterior end; the undulating membrane is usually conspicuous. As with other Nannomonas, T. godfreyi developed in the midgut and proboscis of Glossina and infections matured in 21–28 days in laboratory G. m. morsitans. In The Gambia the normal vertebrate host appears to be the warthog, Phacochoerus aethiopicus, although elsewhere other wild and domestic suids may also be implicated in the life-cycle. T. godfreyi was identified unequivocally using a 380 bp DNA probe specific for a major genomic repeat sequence; its isoenzyme profile distinguished it clearly from T. simiae and three strain groups of T. congolense: savannah, riverine-forest and kilifi.


2015 ◽  
Vol 31 (7) ◽  
pp. 1079-1092 ◽  
Author(s):  
Souraya Boulkour Touioui ◽  
Nadia Zaraî Jaouadi ◽  
Hadjira Boudjella ◽  
Fatma Zohra Ferradji ◽  
Mouna Belhoul ◽  
...  

Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.


Author(s):  
Jason R. Swedlow ◽  
Neil Osheroff ◽  
Tim Karr ◽  
John W. Sedat ◽  
David A. Agard

DNA topoisomerase II is an ATP-dependent double-stranded DNA strand-passing enzyme that is necessary for full condensation of chromosomes and for complete segregation of sister chromatids at mitosis in vivo and in vitro. Biochemical characterization of chromosomes or nuclei after extraction with high-salt or detergents and DNAse treatment showed that topoisomerase II was a major component of this remnant, termed the chromosome scaffold. The scaffold has been hypothesized to be the structural backbone of the chromosome, so the localization of topoisomerase II to die scaffold suggested that the enzyme might play a structural role in the chromosome. However, topoisomerase II has not been studied in nuclei or chromosomes in vivo. We have monitored the chromosomal distribution of topoisomerase II in vivo during mitosis in the Drosophila embryo. This embryo forms a multi-nucleated syncytial blastoderm early in its developmental cycle. During this time, the embryonic nuclei synchronously progress through 13 mitotic cycles, so this is an ideal system to follow nuclear and chromosomal dynamics.


Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
SA Dandlen ◽  
AC Figueiredo ◽  
LG Pedro ◽  
JG Barroso ◽  
MG Miguel ◽  
...  

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