QUALITY OF WASHED PLATELET CONCENTRATE PREPARED WITH A MEMBRANE PLASMA SEPARATOR EC-4A10<sup>®</sup>

We have previously shown that ultrarapid freezing followed by cryomicroscopy provides a uniquely effective preservation of platelet ultrastructure, particularly in its cytoskeleton and membrane systems. However, a limitation of cryomicroscopy of frozen hydrated specimens is the lack of information about the localization of specific proteins within the cell. Methods for immunolocalization of antigens on material that has been rapidly frozen, freeze-substituted and embedded in immunocompatible resins have excited considerable interest among biologists, because of the high quality of preservation afforded by fast-freezing techniques. However, the extent to which fixation and dehydration affect the distribution of antigens is not known. Here we describe a method for imaging immunolabeled cells in the frozen hydrated state, avoiding the destructive steps of dehydration.Blood platelets were isolated from citrated whole blood by differential centrifugation and washed in Hank’s balanced salts. Washed platelet suspensions were placed on formvar coated, carbon stabilized gold finder grids and placed in a humid chamber at 37°C for 30 minutes to allow adhesion and spreading of the cells.

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Preventing adverse reactions in pediatric transfusions using washed platelet concentrate

Pediatrics International ◽

10.1111/ped.14572 ◽

2020 ◽

Author(s):

Ryu Yanagisawa

Keyword(s):

Adverse Reactions ◽

Platelet Concentrate ◽

Washed Platelet

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The quality of MCS+ version C2 double dose platelet concentrate with leucodepletion through a continuous filtration process

Transfusion and Apheresis Science ◽

10.1016/s1473-0502(01)00143-4 ◽

2002 ◽

Vol 26 (1) ◽

pp. 37-41 ◽

Cited By ~ 1

Author(s):

J Seghatchian ◽

P Krailadsiri

Keyword(s):

Platelet Concentrate ◽

Double Dose ◽

Filtration Process ◽

Continuous Filtration

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Effectiveness of washed platelet concentrate and red cell transfusions for a patient with anhaptoglobinemia with antihaptoglobin antibody

Transfusion Medicine ◽

10.1046/j.1365-3148.2002.00350.x ◽

2002 ◽

Vol 12 (1) ◽

pp. 71-73 ◽

Cited By ~ 18

Author(s):

S. Nishiki ◽

M. Hino ◽

T. Kumura ◽

S. Hashimoto ◽

K. Ohta ◽

...

Keyword(s):

Platelet Concentrate ◽

Red Cell ◽

Washed Platelet

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MicroRNAs as a Potential Quality Measurement Tool of Platelet Concentrate Stored in Blood Banks—A Review

Cells ◽

10.3390/cells8101256 ◽

2019 ◽

Vol 8 (10) ◽

pp. 1256 ◽

Cited By ~ 4

Author(s):

Jersey Heitor da Silva Maués ◽

Caroline de Fátima Aquino Moreira-Nunes ◽

Rommel Mário Rodriguez Burbano

Keyword(s):

Molecular Mechanisms ◽

Quality Measurement ◽

Storage Conditions ◽

Platelet Concentrate ◽

Measurement Tool ◽

Blood Component ◽

Public And Private ◽

Platelet Storage ◽

Blood Banks

Background: Platelet concentrate (PC) is one of the main products used in a therapeutic transfusion. This blood component requires special storage at blood banks, however, even under good storage conditions, modifications or degradations may occur and are known as platelet storage lesions. Methods: This research was performed on scientific citation databases PubMed/Medline, ScienceDirect, and Web of Science, for publications containing platelet storage lesions. The results obtained mainly reveal the clinical applicability of miRNAs as biomarkers of storage injury and as useful tools for a problem affecting public and private health, the lack of PC bags in countries with few blood donors. The major studies listed in this review identified miRNAs associated with important platelet functions that are relevant in clinical practice as quality biomarkers of PC, such as miR-223, miR-126, miR-10a, miR-150, miR-16, miR-21, miR-326, miR-495, let-7b, let-7c, let-7e, miR-107, miR-10b, miR-145, miR-155, miR-17, miR-191, miR-197, miR-200b, miR-24, miR-331, miR-376. These miRNAs can be used in blood banks to identify platelet injury in PC bags. Conclusion: The studies described in this review relate the functions of miRNAs with molecular mechanisms that result in functional platelet differences, such as apoptosis. Thus, miRNA profiles can be used to measure the quality of storage PC for more than 5 days, identify bags with platelet injury, and distinguish those with functional platelets.

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