scholarly journals The antisense expression of AhPEPC1 increases seed oil production in peanuts (Arachis hypogaea L.)

2016 ◽  
Vol 67 (4) ◽  
pp. 164 ◽  
Author(s):  
L. Pan ◽  
J. Zhang ◽  
X. Chi ◽  
N. Chen ◽  
M. Chen ◽  
...  

Although phosphoenolpyruvate carboxylases (PEPCs) are reported to be involved in fatty acid accumulation, nitrogen assimilation, and salt and drought stresses, knowledge regarding PEPC gene functions is still limited, particularly in peanuts (Arachis hypogaea L.). In this study, the antisense expression of the peanut PEPC isoform 1 (AhPEPC1) gene increased the lipid content by 5.7%–10.3%. This indicated that AhPEPC1 might be related to plant lipid accumulation. The transgenic plants underwent more root elongation than the wild-type under salinity stress. Additionally, the specific down regulation of the AhPEPC1 gene improved the salt tolerance in peanuts. This is the first report on the role of PEPC in lipid accumulation and salt tolerance in peanuts.

2019 ◽  
Vol 138 (2) ◽  
pp. 325-337 ◽  
Author(s):  
Venkatesh Kandula ◽  
Amareshwari Pudutha ◽  
P. Hima Kumari ◽  
S. Anil Kumar ◽  
P. B. Kavi Kishor ◽  
...  

1994 ◽  
Vol 11 (4) ◽  
pp. 159-162 ◽  
Author(s):  
C. F. van Eeden ◽  
J. B.J van Flensburg ◽  
T. C. de K. van der Linde

2021 ◽  
Vol 13 (6) ◽  
pp. 1
Author(s):  
Weihong Ma ◽  
Xiaoping Zang ◽  
Yuanzheng Liu ◽  
Lixia Wang ◽  
Jiashui Wang ◽  
...  

Fatty acids in avocado fruit (Persea americana Mill.) are vital composition affecting flavour and nutritive value. Hence, horticulturalists are interested in illustrating the functions of transcription factors on fatty acid accumulation in avocado fruit. In the present study, the APETALA2/ethylene-responsive transcription factor gene, PaRAP2.1, was cloned from avocado mesocarp, and the subcellular localization demonstrated that PaRAP2.1 was located in the cytoplasm and nucleus. The PaRAP2.1 was introduced into Arabidopsis thaliana by Agrobacterium-mediated transformation. Furthermore, PaRAP2.1 were functionally verified its effect on fatty acid biosynthesis. Histological analyses of lipid droplets displayed that the striking difference in the lipid droplets in the mature seeds between PaRAP2.1-overexpressing transgenic and wild-type Arabidopsis thaliana lines were revealed based on confocal microscopy images. Subsequently, fatty acid analyses of PaRAP2.1-overexpressing Arabidopsis thaliana lines displayed the significantly higher contents of fatty acids than those in the wild-type plants. Meanwhile, expression amount of ten genes involving in fatty acid biosynthesis dramatically up-regulated in the mature seeds of PaRAP2.1-overexpressing lines than those of wild-type plants. These results provide a theoretical basis for future research in regard to the function of PaRAP2.1 on fatty acid biosynthesis.


2020 ◽  
Vol 152 ◽  
pp. 103560
Author(s):  
Liliana Mercedes Ludueña ◽  
Eliana Bianucci ◽  
María Soledad Anzuay ◽  
Jorge Guillermo Angelini ◽  
Adriana Fabra ◽  
...  

2015 ◽  
Vol 35 (1) ◽  
pp. 31-43 ◽  
Author(s):  
Jing Kong ◽  
Yuanjie Dong ◽  
Yiling Song ◽  
Xiaoying Bai ◽  
Xianyi Tian ◽  
...  

2010 ◽  
Vol 11 (1) ◽  
pp. 27 ◽  
Author(s):  
Joao Augusto ◽  
Timothy B. Brenneman ◽  
Alexander S. Csinos

Peanut (Arachis hypogaea L.) is monocultured in western Nicaragua on loamysand soils, and a pod rot of unknown etiology can greatly reduce crop yield. Pythium myriotylum was frequently isolated from symptomatic pods in fields surveyed at Cosiguina, Leon, and Chinandega regions, although Rhizoctonia and Fusarium were also common. Applications of mefenoxam (0.57 kg a.i./ha), azoxystrobin (0.34 kg a.i./ha), and gypsum (670 kg/ha) at beginning pod and 28 to 35 days later were evaluated in field trials to determine their effects on pod rot and yield. Mefenoxam consistently decreased pod rot incidence and increased yield when disease was severe at Cosiguina. In Leon and Chinandega, azoxystrobin increased yield in fields with little pod rot, apparently by controlling stem rot (Sclerotium rolfsii), but did not decrease pod rot incidence. Application of gypsum had no effect on pod rot incidence or yield, but sometimes increased calcium levels in shells. Pod mycoflora isolations and response to mefenoxam suggest P. myriotylum is the primary cause of peanut pod rot in Nicaragua, especially in Cosiguina, where pod rot incidence was high. Accepted for publication 4 November 2009. Published 15 February 2010.


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